Evaluation of the bilateral cardiac afferent distribution at the spinal and vagal ganglia by retrograde labeling

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dc.authorid0000-0002-6393-1465
dc.authorid0000-0003-0352-1947
dc.authorid0000-0002-7199-583X
dc.contributor.authorAkgül Çağlar, Tuba
dc.contributor.authorDurdu, Zeynep Begüm
dc.contributor.authorTurhan, M. U.
dc.contributor.authorGünal, Mehmet Yalçın
dc.contributor.authorAydın, Mehmet Şerif
dc.contributor.authorÖztürk, Gürkan
dc.contributor.authorÇağavi, Esra
dc.date.accessioned2021-01-14T08:22:56Z
dc.date.available2021-01-14T08:22:56Z
dc.date.issued2021
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.departmentİstanbul Medipol Üniversitesi, Sağlık Bilimleri Enstitüsü, Sinirbilim Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Sağlık Bilimleri Enstitüsü, Tıbbi Biyoloji ve Genetik Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalı
dc.description.abstractThe identity of sensory neurons innervating the heart tissue and the extent of information reported to the brain via these neurons are poorly understood. In order to evaluate the multidimensional distribution and abundance of the cardiac spinal and vagal afferents, we assessed the retrograde labeling efficiency of various tracers, and mapped the cardiac afferents qualitatively and quantitatively at the bilateral nodose ganglia (NGs) and dorsal root ganglia (DRGs). From the five different retrograde tracers evaluated, Di-8-ANEPPQ yielded reproducibly the highest labeling efficiency of cardiac afferents. We demonstrated specific cardiac afferents at NGs and C4 to T11 DRG segments. Next, the 2D reconstruction of the tissue sections and 3D imaging of the whole NGs and DRGs revealed homogeneous and bilateral distribution of cardiac afferents. The quantitative analyses of the labeled cardiac afferents demonstrated approximately 5-6% of the soma in NGs that were equally distributed bilaterally. The neuronal character of Di-8-ANEPPQ labeled cells were validated by coimmunostaning with pan-neuronal marker Tuj-1. In addition, the cell diameters of labeled cardiac sensory neurons were found smaller than 20 mu m, implying the nociceptor phenotype confirmed by co-labeling with TRPV1 and Di-8-ANEPPQ. Importantly, co-labeling with two distinct tracers Di-8-ANEPPQ and WGA-647 demonstrated exclusively the same cardiac afferents in DRGs and NGs, validating our findings. Collectively, our findings revealed the cardiac afferents in NGs bilaterally and DRGs with the highest labeling efficiency reported, spatial distribution and quantitation at both 2D and 3D levels, furthering our understanding of this novel neuron population.
dc.description.sponsorshipIstanbul Medipol Universityen_US
dc.identifier.citationAkgül Çağlar, T., Durdu, Z. B., Turhan, M. U., Günal, M. Y., Aydın, M. Ş., Öztürk, G. ... Çağavi, E. (2021). Evaluation of the bilateral cardiac afferent distribution at the spinal and vagal ganglia by retrograde labeling. Brain Research, 1751. https://dx.doi.org/10.1016/j.brainres.2020.147201
dc.identifier.doi10.1016/j.brainres.2020.147201
dc.identifier.issn0006-8993
dc.identifier.issn1872-6240
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://dx.doi.org/10.1016/j.brainres.2020.147201
dc.identifier.urihttps://hdl.handle.net/20.500.12511/6242
dc.identifier.volume1751
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ecinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/115S381
dc.relation.ispartofBrain Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectDi-8-ANEPPQ
dc.subjectMouse Heart
dc.subjectRetrograde Labeling
dc.subjectCardiac Sensory Neurons
dc.subjectAfferents
dc.subjectMapping
dc.titleEvaluation of the bilateral cardiac afferent distribution at the spinal and vagal ganglia by retrograde labeling
dc.typeArticle

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