Role of mesenchymal stem cells transfected with vascular endothelial growth factor in maintaining renal structure and function in rats with unilateral ureteral obstruction

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dc.authorid0000-0001-5254-2563
dc.contributor.authorÖzbek, Emin
dc.contributor.authorAdaş, Gökhan
dc.contributor.authorÖtünçtemur, Alper
dc.contributor.authorDuruksu, Gökhan
dc.contributor.authorKoç, Bora
dc.contributor.authorPolat, Emre Can
dc.contributor.authorSarvan, Ahu Kemik
dc.contributor.authorOkçu, Alparslan
dc.contributor.authorKamalı, Gülçin
dc.contributor.authorSubaşı, Cansu
dc.contributor.authorKaraöz, Erdal
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:55:59Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:55:59Z
dc.date.issued2015
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Üroloji Ana Bilim Dalı
dc.descriptionWOS: 000356908200009
dc.descriptionPubMed ID: 25542189
dc.description.abstractObjectives: Mesenchymal stem cells hold promise for renal disease treatment. Vascular endothelial growth factor may heal tubule-interstitial fibrosis in unilateral ureteral obstruction by inhibiting epithelial-mesenchymal transition. We investigated the protective effect of vascular endothelial growth factor in transfected mesenchymal stem cells in unilateral ureteral obstruction-induced renal injury in rats. Materials and Methods: Male Wistar Albino rats (32 rats; weight, 250-300 g) were divided into 4 equal groups: group 1, control; group 2, unilateral ureteral obstruction; group 3, unilateral ureteral obstruction and mesenchymal stem cells; and group 4, unilateral ureteral obstruction and vascular endothelial growth factor-transfected mesenchymal stem cells. Vascular endothelial growth factor-transfected mesenchymal stem cells were administered intravenously before onset of unilateral ureteral obstruction. On day 14, the rats were killed and kidneys were retrieved. Tubular necrosis, mono-nuclear cell infiltration, and interstitial fibrosis were evaluated in paraffin blocks. We evaluated green fluorescent protein-positive and vascular endothelial growth factor-positive cells; anti-inflammatory (Prostaglandin E2 Receptor) and interleukin 1 receptor antagonist), proinflammatory/anti-inflammatory (interleukin 6), and proinflammatory (MPO) cytokine expression levels; and levels of nitric oxide; transforming growth factor beta 1, E-cadherin, and hydroxyproline. Results: Green fluorescent protein-positive cells were negative in the renal parenchyma in groups 1 and 2 and positive in groups 3 and 4. Vascular endothelial growth factor levels were significantly higher in group 4. Transforming growth factor 131, nitric oxide, and E-cadherin levels were significantly higher in the unilateral ureteral obstruction than control group; however, in the study groups, these values were not significantly different from the unilateral ureteral obstruction group. In stem cell-transplanted tissue samples, EP3, interleukin 1 receptor antagonist, and interleukin 6 levels were elevated, but MPO expression levels were low. Although there were significant differences for tubular necrosis and fibrosis in group 2, there were significant reductions in tubular injury and fibrosis in groups 3 and 4. Conclusions: Systemic stem cells transplanted into the kidney protected against unilateral ureteral obstruction-induced renal epithelial-mesenchymal transition and renal fibrosis.
dc.identifier.citationÖzbek, E., Adaş, G., Ötünçtemur, A., Duruksu, G., Koç, B., Polat, E. C. ... Karaöz, E. (2015). Role of mesenchymal stem cells transfected with vascular endothelial growth factor in maintaining renal structure and function in rats with unilateral ureteral obstruction. Experimental and Clinical Transplantation, 13(3), 262-272. https://dx.doi.org/10.6002/ect.2014.0080
dc.identifier.doi10.6002/ect.2014.0080
dc.identifier.endpage272
dc.identifier.issn1304-0855
dc.identifier.issue3
dc.identifier.scopusqualityQ3
dc.identifier.startpage262
dc.identifier.urihttps://dx.doi.org/10.6002/ect.2014.0080
dc.identifier.urihttps://hdl.handle.net/20.500.12511/2505
dc.identifier.volume13
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBaskent University
dc.relation.ispartofExperimental and Clinical Transplantationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectEpithelial-Mesenchymal Transition
dc.subjectRenal Injury
dc.subjectObstructive Nephropathy
dc.subjectUreter Obstruction
dc.titleRole of mesenchymal stem cells transfected with vascular endothelial growth factor in maintaining renal structure and function in rats with unilateral ureteral obstruction
dc.typeArticle

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