Inhibition of cathepsin-K and matrix metalloproteinase by photodynamic therapy

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dc.authorid0000-0001-8833-5259
dc.contributor.authorKara, Özlem
dc.contributor.authorŞeşeoğulları Dirihan, Roda
dc.contributor.authorSayın Özel, Gülsüm
dc.contributor.authorTezvergil Mutluay, Arzu
dc.contributor.authorÜşümez, Aslıhan
dc.date.accessioned2021-10-13T12:49:52Z
dc.date.available2021-10-13T12:49:52Z
dc.date.issued2021
dc.departmentİstanbul Medipol Üniversitesi, Diş Hekimliği Fakültesi, Protetik Diş Tedavisi Ana Bilim Dalı
dc.description.abstractObjectives: The objective of this study was to determine the effects of antimicrobial photodynamic therapy (aPDT) with indocyanine green (ICG) and toluidine blue (TB) on protease activity (matrix-bound cathepsin K and matrix metalloproteinase (MMP) and dentin bond strength. Methods: Caries-free human third molars were assigned to five groups: 1—control group, 2—application of ICG with activation using an 810 nm diode (aPDT), 3—application of ICG, 4—application of TB with activation using a 660 nm diode (aPDT), and 5—application of TB. For the enzymatic investigation, dentin beams were incubated for either 3 days or 3 weeks. Aliquots of the incubation media were analyzed by ELISA for CTX (C-terminal cross-linked telopeptide of type I Collagen) and ICTP (cross-linked carboxy-terminal telopeptide of type I collagen). For microtensile bond strength testing (?TBS), composite resins were layered onto the tooth surface; the samples were then subjected to ?TBS. Kruskall–Wallis and Mann–Whitney U tests were applied for statistical analysis of CTX and ICTP, one way-ANOVA and Tukey's test were applied for statistical analysis of ?TBS. Results: Pretreating the dentin matrices with aPDT decreased the endogenous protease activity. ICG with laser activation resulted in the highest ?TBS. Therefore, aPDT should be considered as a treatment method because it can reduce MMP-mediated dentin degradation and increase the ?TBS. Significance: Inhibiting endogenous protease activity improves the stability of the dentin–adhesive bond and the durability of the bond strength.
dc.identifier.citationKara, Ö., Şeşeoğulları Dirihan, R., Sayın Özel, G., Tezvergil Mutluay, A. ve Üşümez, A. (2021). Inhibition of cathepsin-K and matrix metalloproteinase by photodynamic therapy. Dental Materials, 37(10), e485-e492. https://dx.doi.org/10.1016/j.dental.2021.08.015
dc.identifier.doi10.1016/j.dental.2021.08.015
dc.identifier.endpagee492
dc.identifier.issn0109-5641
dc.identifier.issn1879-0097
dc.identifier.issue10
dc.identifier.scopusqualityQ1
dc.identifier.startpagee485
dc.identifier.urihttps://dx.doi.org/10.1016/j.dental.2021.08.015
dc.identifier.urihttps://hdl.handle.net/20.500.12511/8443
dc.identifier.volume37
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Inc.
dc.relation.ispartofDental Materialsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectAntimicrobial Photodynamic Therapy
dc.subjectCTX
dc.subjectICTP
dc.subjectIndocyanine Green
dc.subjectMicrotensile Bond Strength
dc.subjectToluidine Blue
dc.titleInhibition of cathepsin-K and matrix metalloproteinase by photodynamic therapy
dc.typeArticle

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