Detailed tail proteomic analysis of axolotl (Ambystoma mexicanum) using an mRNA-seq reference database

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dc.authorid0000-0002-7059-1884
dc.authorid0000-0003-0352-1947
dc.contributor.authorDemircan, Turan
dc.contributor.authorKeskin, İlknur
dc.contributor.authorDumlu, Seda Nilgün
dc.contributor.authorAytürk, Nilüfer
dc.contributor.authorAvşaroğlu, Mahmut Erhan
dc.contributor.authorAkgün, Emel
dc.contributor.authorÖztürk, Gürkan
dc.contributor.authorBaykal, Ahmet Tarık
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:02:41Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:02:41Z
dc.date.issued2017
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Bilgisayar Mühendisliği Bölümü
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.descriptionWOS: 000394432900012
dc.descriptionPubMed ID: 27896924
dc.description.abstractSalamander axolotl has been emerging as an important model for stem cell research due to its powerful regenerative capacity. Several advantages, such as the high capability of advanced tissue, organ, and appendages regeneration, promote axolotl as an ideal model system to extend our current understanding on the mechanisms of regeneration. Acknowledging the common molecular pathways between amphibians and mammals, there is a great potential to translate the messages from axolotl research to mammalian studies. However, the utilization of axolotl is hindered due to the lack of reference databases of genomic, transcriptomic, and proteomic data. Here, we introduce the proteome analysis of the axolotl tail section searched against an mRNA-seq database. We translated axolotl mRNA sequences to protein sequences and annotated these to process the LC-MS/MS data and identified 1001 nonredundant proteins. Functional classification of identified proteins was performed by gene ontology searches. The presence of some of the identified proteins was validated by in situ antibody labeling. Furthermore, we have analyzed the proteome expressional changes postamputation at three time points to evaluate the underlying mechanisms of the regeneration process. Taken together, this work expands the proteomics data of axolotl to contribute to its establishment as a fully utilized model.
dc.identifier.citationDemircan, T., Keskin, İ., Dumlu, S. N., Aytürk, N., Avşaroğlu, M. E., Akgün, E. ... Baykal, A. T. (2017). Detailed tail proteomic analysis of axolotl (Ambystoma mexicanum) using an mRNA-seq reference database. Proteomics, 17(1-2). https://dx.doi.org/10.1002/pmic.201600338
dc.identifier.doi10.1002/pmic.201600338
dc.identifier.issn1615-9853
dc.identifier.issn1615-9861
dc.identifier.issue45323
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://dx.doi.org/10.1002/pmic.201600338
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3709
dc.identifier.volume17
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofProteomicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAnimal Proteomics
dc.subjectAxolotl
dc.subjectTail Regeneration
dc.titleDetailed tail proteomic analysis of axolotl (Ambystoma mexicanum) using an mRNA-seq reference database
dc.typeArticle

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