Microbial composition of Kombucha determined using amplicon sequencing and shotgun metagenomics

Arıkan, Muzaffer; Mitchell, Alex L.; Finn, Robert D.; Gürel, Filiz

Microbial composition of Kombucha determined using amplicon sequencing and shotgun metagenomics

dc.authorid	0000-0001-5162-2000
dc.contributor.author	Arıkan, Muzaffer
dc.contributor.author	Mitchell, Alex L.
dc.contributor.author	Finn, Robert D.
dc.contributor.author	Gürel, Filiz
dc.date.accessioned	2020-07-13T11:02:20Z
dc.date.available	2020-07-13T11:02:20Z
dc.date.issued	2020
dc.department	İstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.description.abstract	Kombucha, a fermented tea generated from the co-culture of yeasts and bacteria, has gained worldwide popularity in recent years due to its potential benefits to human health. As a result, many studies have attempted to characterize both its biochemical properties and microbial composition. Here, we have applied a combination of whole metagenome sequencing (WMS) and amplicon (16S rRNA and Internal Transcribed Spacer 1 [ITS1]) sequencing to investigate the microbial communities of homemade Kombucha fermentations from day 3 to day 15. We identified the dominant bacterial genus as Komagataeibacter and dominant fungal genus as Zygosaccharomyces in all samples at all time points. Furthermore, we recovered three near complete Komagataeibacter genomes and one Zygosaccharomyces bailii genome and then predicted their functional properties. Also, we determined the broad taxonomic and functional profile of plasmids found within the Kombucha microbial communities. Overall, this study provides a detailed description of the taxonomic and functional systems of the Kombucha microbial community. Based on this, we conject that the functional complementarity enables metabolic cross talks between Komagataeibacter species and Z. bailii, which helps establish the sustained a relatively low diversity ecosystem in Kombucha.
dc.description.sponsorship	Scientific Research Projects Coordination Unit of Istanbul Univ.	en_US
dc.description.sponsorship	Biotechnology and Biological Sciences Research Council (BBSRC)	en_US
dc.description.sponsorship	European Molecular Biology Laboratory	en_US
dc.identifier.citation	Arıkan, M., Mitchell, A. L., Finn, R. D. ve Gürel, F. (2020). Microbial composition of Kombucha determined using amplicon sequencing and shotgun metagenomics. Journal of Food Science, 85(2), 455-464. https://dx.doi.org/10.1111/1750-3841.14992
dc.identifier.doi	10.1111/1750-3841.14992
dc.identifier.endpage	464
dc.identifier.issn	0022-1147
dc.identifier.issn	1750-3841
dc.identifier.issue	2
dc.identifier.scopusquality	Q1
dc.identifier.startpage	455
dc.identifier.uri	https://dx.doi.org/10.1111/1750-3841.14992
dc.identifier.uri	https://hdl.handle.net/20.500.12511/5469
dc.identifier.volume	85
dc.identifier.wosquality	Q2
dc.indekslendigikaynak	Web of Science
dc.indekslendigikaynak	Scopus
dc.indekslendigikaynak	PubMed
dc.language.iso	en
dc.publisher	Wiley
dc.relation.ispartof	Journal of Food Science	en_US
dc.relation.publicationcategory	Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.relation.tubitak	info:eu-repo/grantAgreement/TUBITAK/SOBAG/215Z672
dc.rights	Attribution 4.0 International	*
dc.rights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject	Kombucha
dc.subject	Shotgun Metagenomics
dc.subject	Fermented Tea
dc.subject	16S
dc.subject	ITS
dc.title	Microbial composition of Kombucha determined using amplicon sequencing and shotgun metagenomics
dc.type	Article