Two distinct types of dying back axonal degeneration in vitro

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dc.authorid0000-0003-0352-1947
dc.contributor.authorÖztürk, Gürkan
dc.contributor.authorCengiz, Nurettin
dc.contributor.authorErdoğan, Ender
dc.contributor.authorHim, Aydın
dc.contributor.authorOğuz Kaval, Elif
dc.contributor.authorYenidünya, E.
dc.contributor.authorAyşit, Neşe
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:02:00Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:02:00Z
dc.date.issued2013
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.descriptionWOS: 000318425900004
dc.descriptionPubMed ID: 22845867
dc.description.abstractAims: In many neurodegenerative diseases and following traumas, dying back degeneration is a common phenomenon that aggravates the pathology and may eventually lead to death of the affected neurone. We aimed to investigate the mechanism of dying back degeneration with an in vitro axonal injury model. Methods: We cultured adult mouse dorsal root ganglion neurones and with a precise laser beam, cut the axons they extended. Preparations were imaged continuously and images were analysed to describe and quantify ensuing events. Potential contributions of calpains and caspases to the degeneration were explored using specific inhibitors and immunohistochemistry. In vivo implications of the results were sought in nerve sections after sciatic nerve cut. Results: The proximal part of the transected axons went under basically two types of dying back degeneration, fragmentation and retraction. In fragmentation the cytoplasm became condensed and with concomitant axial collapse the axon disintegrated into small pieces. In retraction, the severed axon was pulled back to the soma in an organized manner. We demonstrated that fragmentation was associated with a high risk of cell death, while survival rate with retraction was as high as those of uninjured neurones. Regeneration of transected axon was more likely after retraction than following fragmentation. Activities of caspase-3 and calpains but not of caspase-6 were found linked with retraction and regeneration but not with the fragmentation. Conclusions: This study describes two quite distinct types of dying back degeneration that lead an injured neurone to quite different fates.
dc.description.sponsorshipYuzuncu Yil University Directorate of Scientific Research Projects [TF073]en_US
dc.description.sponsorshipThis study was supported by Yuzuncu Yil University Directorate of Scientific Research Projects with the grant TF073.en_US
dc.identifier.citationÖztürk, G., Cengiz, N., Erdoğan, E., Him, A., Oğuz Kaval, E., Yenidünya, E. ve Ayşit, N. (2013). Two distinct types of dying back axonal degeneration in vitro. Neuropathology And Applied Neurobiology, 39(4), 362-376. https://dx.doi.org/10.1111/j.1365-2990.2012.01295.x
dc.identifier.doi10.1111/j.1365-2990.2012.01295.x
dc.identifier.endpage376
dc.identifier.issn0305-1846
dc.identifier.issn1365-2990
dc.identifier.issue4
dc.identifier.scopusqualityQ1
dc.identifier.startpage362
dc.identifier.urihttps://dx.doi.org/10.1111/j.1365-2990.2012.01295.x
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3520
dc.identifier.volume39
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofNeuropathology And Applied Neurobiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectAxotomy
dc.subjectDaspase
dc.subjectDegeneration
dc.subjectDying Back
dc.subjectLaser
dc.subjectNeurone Death
dc.titleTwo distinct types of dying back axonal degeneration in vitro
dc.typeArticle

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