Two phases of macrophages: Inducing maturation and death of oligodendrocytes in vitro co-culture

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dc.authorid0000-0001-7266-9844
dc.authorid0000-0003-1106-3288
dc.contributor.authorAydınlı, Fatmagül İlayda
dc.contributor.authorEr, Sezgin
dc.contributor.authorKerman, Bilal Ersen
dc.date.accessioned2022-11-04T07:40:25Z
dc.date.available2022-11-04T07:40:25Z
dc.date.issued2022
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.description.abstractBackground: The plasticity of macrophages in the immune response is a dynamic situation dependent on external stimuli. The activation of macrophages both has beneficial and detrimental effects on mature oligodendrocytes (OLs) and myelin. The activation towards inflammatory macrophages has a critical role in the immune-mediated oligodendrocytes death in multiple sclerosis (MS) lesions. New method: We established an in vitro co-culture method to study the function of macrophages in the survival and maturation of OLs. Results: We revealed that M1 macrophages decreased the number of mature OLs and phagocytosed the myelin. Interestingly, non-activated as well as M2 macrophages contributed to an increase in the number of mature OLs in our in vitro co-culture platform. Comparison with existing methods: We added an antibody against an OL surface antigen in our in vitro co-cultures. The antibody presents the OLs to the macrophages enabling the investigation of direct interactions between macrophages and OLs. Conclusion: Our co-culture system is a feasible method for the investigation of the direct cell-to-cell interactions between OLs and macrophages. We utilized it to show that M2 and non-activated macrophages may be employed to enhance remyelination.
dc.identifier.citationAydınlı, F. İ., Er, S. ve Kerman, B. E. (2022). Two phases of macrophages: Inducing maturation and death of oligodendrocytes in vitro co-culture. Journal of Neuroscience Methods, 382. https://doi.org/10.1016/j.jneumeth.2022.109723
dc.identifier.doi10.1016/j.jneumeth.2022.109723
dc.identifier.issn0165-0270
dc.identifier.issn1872-678X
dc.identifier.pmid36207003
dc.identifier.scopus2-s2.0-85140801513
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1016/j.jneumeth.2022.109723
dc.identifier.urihttps://hdl.handle.net/20.500.12511/9919
dc.identifier.volume382
dc.identifier.wos000880389900003en_US
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAydınlı, Fatmagül İlayda
dc.institutionauthorEr, Sezgin
dc.institutionauthorKerman, Bilal Ersen
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Neuroscience Methodsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectMultiple Sclerosis
dc.subjectOligodendrocytes
dc.subjectPeritoneal Macrophages
dc.subjectRemyelination
dc.titleTwo phases of macrophages: Inducing maturation and death of oligodendrocytes in vitro co-culture
dc.typeArticle

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