Downregulation of Yap1 during limb regeneration results in defective bone formation in axolotl

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dc.authorid0000-0001-8089-1330
dc.authorid0000-0003-0352-1947
dc.contributor.authorBay, Sadık
dc.contributor.authorÖztürk, Gürkan
dc.contributor.authorEmekli, Nesrin
dc.contributor.authorDemircan, Turan
dc.date.accessioned2023-06-19T08:23:06Z
dc.date.available2023-06-19T08:23:06Z
dc.date.issued2023
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.description.abstractThe Hippo pathway plays an imperative role in cellular processes such as differentiation, regeneration, cell migration, organ growth, apoptosis, and cell cycle. Transcription coregulator component of Hippo pathway, YAP1, promotes transcription of genes involved in cell proliferation, migration, differentiation, and suppressing apoptosis. However, its role in epimorphic regeneration has not been fully explored. The axolotl is a well-established model organism for developmental biology and regeneration studies. By exploiting its remarkable regenerative capacity, we investigated the role of Yap1 in the early blastema stage of limb regeneration. Depleting Yap1 using gene-specific morpholinos attenuated the competence of axolotl limb regeneration evident in bone formation defects. To explore the affected downstream pathways from Yap1 down-regulation, the gene expression profile was examined by employing LC-MS/MS technology. Based on the generated data, we provided a new layer of evidence on the putative roles of increased protease inhibition and immune system activities and altered ECM composition in diminished bone formation capacity during axolotl limb regeneration upon Yap1 deficiency. We believe that new insights into the roles of the Hippo pathway in complex structure regeneration were granted in this study.
dc.description.sponsorshipScience Academyen_US
dc.identifier.citationBay, S., Öztürk, G., Emekli, N. ve Demircan, T. (2023). Downregulation of Yap1 during limb regeneration results in defective bone formation in axolotl. Developmental Biology, 500, 31-39. https://doi.org/10.1016/j.ydbio.2023.06.001
dc.identifier.doi10.1016/j.ydbio.2023.06.001
dc.identifier.endpage39
dc.identifier.issn0012-1606
dc.identifier.issn1095-564X
dc.identifier.pmid37271360
dc.identifier.scopus2-s2.0-85161015865
dc.identifier.scopusqualityQ2
dc.identifier.startpage31
dc.identifier.urihttps://doi.org/10.1016/j.ydbio.2023.06.001
dc.identifier.urihttps://hdl.handle.net/20.500.12511/11103
dc.identifier.volume500
dc.identifier.wos001017983900001en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorBay, Sadık
dc.institutionauthorÖztürk, Gürkan
dc.institutionauthorEmekli, Nesrin
dc.language.isoen
dc.publisherElsevier Inc.
dc.relation.ispartofDevelopmental Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/119Z976
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectYAP1
dc.subjectHippo Pathway
dc.subjectAxolotl
dc.subjectRegeneration
dc.subjectProteomics
dc.subjectBone Formation Defect
dc.titleDownregulation of Yap1 during limb regeneration results in defective bone formation in axolotl
dc.typeArticle

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