Proteomic study of the microdissected aortic media in human thoracic aortic aneurysms

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dc.authorid0000-0002-8814-7351
dc.contributor.authorSerhatlı, Müge
dc.contributor.authorBaysal, Kemal
dc.contributor.authorAçılan, Ceyda
dc.contributor.authorTuncer, Eylem
dc.contributor.authorBekpınar, Seldağ
dc.contributor.authorBaykal, Ahmet Tarık
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:01:29Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:01:29Z
dc.date.issued2014
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.descriptionWOS: 000344636500052
dc.descriptionPubMed ID: 25264617
dc.description.abstractAortic aneurysm is a complex multifactorial disease, and its molecular mechanism is not understood. In thoracic aortic aneurysm (TAA), the expansion of the aortic wall is lead by extracellular matrix (ECM) degeneration in the medial layer, which leads to weakening of the aortic wall. This dilatation may end in rupture andif untreateddeath. The aortic media is composed of vascular smooth muscle cells (VSMCs) and proteins involved in aortic elasticity and distensibility. Delineating their functional and quantitative decrease is critical in elucidating the disease causing mechanisms as well as the development of new preventive therapies. Laser microdissection (LMD) is an advanced technology that enables the isolation of the desired portion of tissue or cells for proteomics analysis, while preserving their integrity. In our study, the aortic media layers of 36 TAA patients and 8 controls were dissected using LMD technology. The proteins isolated from these tissue samples were subjected to comparative proteomic analysis by nano-LCMS/MS, which enabled the identification of 352 proteins in aortic media. Among these, 41 proteins were differentially expressed in the TAA group with respect to control group, and all were downregulated in the patients. Of these medial proteins, 25 are novel, and their association with TAA is reported for the first time in our study. Subsequent analysis of the data by ingenuity pathway analysis (IPA) shows that the majority of differentially expressed proteins were found to be cytoskeletal-associated proteins and components of the ECM which are critical in maintaining aortic integrity. Our results indicate that the protein expression profile in the aortic media from TAA patients differs significantly from controls. Further analysis of the mechanism points to markers of pathological ECM remodeling, which, in turn, affect VSMC cytosolic structure and architecture. In the future, the detailed investigation of the differentially expressed proteins may provide insight into the elucidation of the pathological processes underlying aneurysms.
dc.description.sponsorshipIstanbul University [11537]; EU [Health-F2-2008-200647]; TUBITAK Marmara Research Center, Genetic Engineering and Biotechnology Instituteen_US
dc.description.sponsorshipThis work has been supported by The Research Fund of Istanbul University (project no. 11537), EU 7th Framework Program project; Fighting Aneurysmal Disease (Health-F2-2008-200647) and TUBITAK Marmara Research Center, Genetic Engineering and Biotechnology Institute.en_US
dc.identifier.citationSerhatlı, M., Baysal, K., Açılan, C., Tuncer, E., Bekpınar, S. ve Baykal, A. T. 82014). Proteomic study of the microdissected aortic media in human thoracic aortic aneurysms. Journal of Proteome Research, 13(11), 5071-5080. https://dx.doi.org/10.1021/pr5006586
dc.identifier.doi10.1021/pr5006586
dc.identifier.endpage5080
dc.identifier.issn1535-3893
dc.identifier.issn1535-3907
dc.identifier.issue11
dc.identifier.scopusqualityQ1
dc.identifier.startpage5071
dc.identifier.urihttps://dx.doi.org/10.1021/pr5006586
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3300
dc.identifier.volume13
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.relation.ispartofJournal of Proteome Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectThoracic Aortic Aneurysm
dc.subjectLaser Capture Microdissection
dc.subjectLabel-Free Proteomics
dc.subjectProtein Expression
dc.subjectVascular Smooth Muscle
dc.titleProteomic study of the microdissected aortic media in human thoracic aortic aneurysms
dc.typeArticle

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