Does oseltamivir protect human chondrocyte and nucleus pulposus cells from degeneration by inhibiting senescence and proinflammation mediated by the NLRP3 inflammasome and NF-?B?

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dc.authorid0000-0001-7392-7729
dc.authoridQ3
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorAkalan, Hande
dc.contributor.authorÖznam, Kadir
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorYaşar Şirin, Duygu
dc.contributor.authorÖzbek, Hanefi
dc.date.accessioned2022-07-29T11:50:27Z
dc.date.available2022-07-29T11:50:27Z
dc.date.issued2022
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Ortopedi ve Travmatoloji Ana Bilim Dalı
dc.description.abstractOBJECTIVE: Recent drug design studies suggest that inflammation is among the most important factors in the development of both intervertebral disc (IVD) degeneration (IVDD) and osteoarthritis (OA) due to cartilage damage. This study aimed to investigate whether the anti-inflammatory drug oseltamivir has a toxic effect on IVD and cartilage tissue cells. It assessed what effect oseltamivir has on hypoxia-inducible factor (HIF)-1 alpha (HIF1?), which plays an important role in anabolic pathways in IVD and cartilage tissue. In addition, the study analyzed whether oseltamivir could inhibit the release of inflammatory interleukin-1 beta (IL-1?) via the nuclear factor kappa-B (NF-?B) signaling pathway by activating the nucleotide-binding oligomerization domain and leucine-rich repeat protein-3 (NLRP3) inflammasome. MATERIALS AND METHODS: Human lumbar IVD (n = 8) tissues were isolated for annulus fibrosus (AF) and nucleus pulposus (NP) primary cell cultures, and human tibial and femoral cartilage tissues (n = 8) were isolated for primary chondrocyte cultures. Untreated groups served as the control and oseltamivir-treated groups as the study sample. Cell viability and cytotoxicity were evaluated at 0, 24, 48, and 72 h in all groups for changes in HIF-1?, IL-1?, NF-?B, and the NLRP3-inflammasome protein expressions using Western blotting. The ? significance value was < 0.05. RESULTS: In the oseltamivir-treated groups, cell proliferation decreased in both AF/NP cell and chondrocyte cultures obtained from IVD cartilage tissues. After Western blotting analysis, changes were observed in the protein expressions of HIF-1?, IL-1?, NF-?B, and the NLRP3 inflammasome in both AF/NP cells and chondrocytes. The results were statistically significant (p < 0.05). CONCLUSIONS: Oseltamivir treatment may be a promising regenerative strategy to manage IVDD and osteoarthritic cartilage tissues.
dc.identifier.citationYılmaz, İ., Akalan, H., Öznam, K., Karaarslan, N., Yaşar Şirin, D. ve Özbek, H. (2022). Does oseltamivir protect human chondrocyte and nucleus pulposus cells from degeneration by inhibiting senescence and proinflammation mediated by the NLRP3 inflammasome and NF-?B? European Review for Medical and Pharmacological Sciences, 26(13), 4816-4827. https://doi.org/10.26355/eurrev_202207_29207
dc.identifier.doi10.26355/eurrev_202207_29207
dc.identifier.endpage4827
dc.identifier.issn1128-3602
dc.identifier.issue13
dc.identifier.pmid35856374
dc.identifier.scopus2-s2.0-85134249183
dc.identifier.scopusqualityQ2
dc.identifier.startpage4816
dc.identifier.urihttps://doi.org/10.26355/eurrev_202207_29207
dc.identifier.urihttps://hdl.handle.net/20.500.12511/9625
dc.identifier.volume26
dc.identifier.wos000837256500034en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖznam, Kadir
dc.language.isoen
dc.publisherVerduci Editore s.r.l
dc.relation.ispartofEuropean Review for Medical and Pharmacological Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectDegeneration
dc.subjectInflammation
dc.subjectNF-?B
dc.subjectNLRP3 Inflammasome
dc.subjectOseltamivir
dc.titleDoes oseltamivir protect human chondrocyte and nucleus pulposus cells from degeneration by inhibiting senescence and proinflammation mediated by the NLRP3 inflammasome and NF-?B?
dc.title.alternativeDoes oseltamivir protect human chondrocyte and nucleus pulposus cells from degeneration by inhibiting senescence and proinflammation mediated by the NLRP3 inflammasome and NF-kappa B?
dc.title.alternativeDoes oseltamivir protect human chondrocyte and nucleus pulposus cells from degeneration by inhibiting senescence and proinflammation mediated by the NLRP3 inflammasome and NF-kappa B?
dc.typeArticle

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