Changes in secondary structure of protein in skeletal muscle due to high-carbohydrate or high-fat diets

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dc.contributor.authorEzer Özer, Nazlı
dc.contributor.authorDoğan Mollaoğlu, Ayça
dc.date.accessioned2025-12-11T06:27:56Z
dc.date.available2025-12-11T06:27:56Z
dc.date.issued2024
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Biyofizik Ana Bilim Dalı
dc.description.abstractObjective: Obesity, which arises from changes in lifestyle and feeding habits, poses a threat to human health. One essential contributor to the increase in obesity rates is the popularity of high-calorie diets. This study aims to investigate high-fat (HFD) and high-carbohydrate (HCD) diet-induced molecular changes in protein secondary structure in longissimus dorsi skeletal muscle tissues of female inbred C57BL/6J mice by utilizing Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy. Materials and Methods: Mice were fed a control diet, HCD, or HFD for 24 weeks. Their skeletal muscle tissues were collected, and their spectra were recorded using a Bruker Invenio S ATR-FTIR spectrometer in the 4000-400 cm-1 region. Results: The protein secondary structure profiles of the HCD group demonstrated a significant rise in antiparallel beta-sheet and beta-turn and a decline in parallel beta-sheets, together with the insignificant increase in aggregated beta-sheets and a decrease in alpha-helix. The impact of an HFD on protein conformation is less pronounced than HCD. The HFD diet led to an increase in antiparallel beta-sheets and a decrease in parallel beta sheets. Although insignificant, an increase was observed in beta-turn and alpha-helix. Conclusion: These results propose the appearance of protein aggregation and/or formation of protein- protein intermolecular interaction in skeletal muscle tissues of female inbred C57BL/6J mice. Collectively, these data suggest that both high-calorie diets impair secondary structures of protein in skeletal muscle that may affect its metabolic function.
dc.description.sponsorshipTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)
dc.identifier.citationEzer Özer, N. ve Doğan Mollaoğlu, A. (2024). Changes in secondary structure of protein in skeletal muscle due to high-carbohydrate or high-fat diets. Meandros Medical and Dental Journal, 25(3), 233-243. http://dx.doi.org/10.69601/meandrosmdj.1537978
dc.identifier.doi10.69601/meandrosmdj.1537978
dc.identifier.endpage243
dc.identifier.issn2149-9063
dc.identifier.issue3
dc.identifier.startpage233
dc.identifier.urihttp://dx.doi.org/10.69601/meandrosmdj.1537978
dc.identifier.urihttps://hdl.handle.net/20.500.12511/13324
dc.identifier.volume25
dc.identifier.wosWOS:001378426300001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.institutionauthorEzer Özer, Nazlı
dc.institutionauthorid0000-0002-2313-4218
dc.language.isoen
dc.relation.ecinfo:eu-repo/grantAgreement/TUBITAK/3501/SBAG/118S484
dc.relation.ispartofMeandros Medical and Dental Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsAttribution-NonCommercial 4.0 International
dc.subjectObesity
dc.subjectHigh-Carbohydrate Diet
dc.subjectHigh-Fat Diet
dc.subjectProtein Secondary Structure
dc.subjectFTIR
dc.subjectNATURAL SCIENCES::Chemistry::Physical chemistry::Spectroscopy
dc.titleChanges in secondary structure of protein in skeletal muscle due to high-carbohydrate or high-fat diets
dc.typeArticle

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