In vitro synergistic effect and mutant prevention concentrations of cefepime alone or in combination with sulbactam against OXA-48-positiveKlebsiella pneumoniaeIsolates
| dc.authorid | 0000-0001-8022-7325 | |
| dc.contributor.author | Mataracı Kara, Emel | |
| dc.contributor.author | Yılmaz, Mesut | |
| dc.contributor.author | Özbek Çelik, Berna | |
| dc.date.accessioned | 2020-10-26T07:24:06Z | |
| dc.date.available | 2020-10-26T07:24:06Z | |
| dc.date.issued | 2020 | |
| dc.department | İstanbul Medipol Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Ana Bilim Dalı | |
| dc.description.abstract | The aim of this study is to investigate the combination of cefepime and sulbactam. Sulbactam, when administered , will effectively inhibit all Extended-spectrum beta lactamases (ESBLs) of the microorganism, while cefepime will inhibit the growth of the resistant microorganisms since it will not be hydrolyzed by OXA-48. Forty OXA-48-producingK. pneumoniaestrains were investigated for their Minimum inhibitory concentrations (MICs) for carbapenems, cefepime, and cefepime + sulbactam by broth microdilution method. Also, the mutant prevention concentration (MPC)s of cefepime alone or in combination with sulbactam was determined. Additionally, the bactericidal activities of cefepime and cefepime + sulbactam were evaluated by the time-kill curve (TKC) assay against selected strains. Also, the in vitro synergistic activity of cefepime + sulbactam combination was determined by TKC. Based on MIC results, up to 35/40 and 34/40 of the strains were resistant to carbapenems and cefepime, respectively. Cefepime + sulbactam MIC range was lower than those for cefepime alone against all the studied isolates. Moreover, cefepime + sulbactam combination presented lower MPC values than cefepime alone. The synergistic interactions of cefepime + sulbactam were also achieved against studied strains at 24 h. No antagonism was observed against studiedK. pneumoniaestrains. The findings of this study displayed that cefepime + sulbactam combination had synergistic or additive effect against OXA-48-producingK. pneumoniaestrains. Additionally, it was first observed that this combination could display a lower MPC than cefepime alone. Further investigations may be helpful for understanding the effectiveness of cefepime + sulbactam combinations for OXA-48-positive carbapenem-resistantK. pneumoniaeisolates. | |
| dc.description.sponsorship | Istanbul University | en_US |
| dc.identifier.citation | Mataracı Kara, E., Yılmaz, M. ve Özbek Çelik, B. (2020). In vitro synergistic effect and mutant prevention concentrations of cefepime alone or in combination with sulbactam against OXA-48-positiveKlebsiella pneumoniaeIsolates. Current Microbiology, 77(9), 2137-2142. https://dx.doi.org/10.1007/s00284-020-02094-0 | |
| dc.identifier.doi | 10.1007/s00284-020-02094-0 | |
| dc.identifier.endpage | 2142 | |
| dc.identifier.issn | 0343-8651 | |
| dc.identifier.issn | 1432-0991 | |
| dc.identifier.issue | 9 | |
| dc.identifier.scopusquality | Q2 | |
| dc.identifier.startpage | 2137 | |
| dc.identifier.uri | https://dx.doi.org/10.1007/s00284-020-02094-0 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12511/5968 | |
| dc.identifier.volume | 77 | |
| dc.identifier.wosquality | Q4 | |
| dc.indekslendigikaynak | Web of Science | |
| dc.indekslendigikaynak | Scopus | |
| dc.indekslendigikaynak | PubMed | |
| dc.language.iso | en | |
| dc.publisher | Springer | |
| dc.relation.ispartof | Current Microbiology | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | |
| dc.rights | info:eu-repo/semantics/embargoedAccess | |
| dc.subject | OXA-48-positiveKlebsiella | |
| dc.subject | Sulbactam Against | |
| dc.subject | Cefepime Alone | |
| dc.title | In vitro synergistic effect and mutant prevention concentrations of cefepime alone or in combination with sulbactam against OXA-48-positiveKlebsiella pneumoniaeIsolates | |
| dc.type | Article |
