Cytotoxicity of standardized curcuminoids mixture against epithelial ovarian cancer cell line SKOV-3

Basit Öğe Kaydı
dc.authorid0000-0001-9434-3861
dc.contributor.authorAlmosa, Heba
dc.contributor.authorAlqriqri, Mihal
dc.contributor.authorDenetiu, Iuliana
dc.contributor.authorBaghdadi, Mohammed Abbas
dc.contributor.authorAlkhaled, Mohammed
dc.contributor.authorAlhosin, Mahmoud
dc.contributor.authorAldajani, Wejdan A.
dc.contributor.authorZamzami, Mazin
dc.contributor.authorÜçışık, Mehmet Hikmet
dc.date.accessioned2020-08-05T07:03:47Z
dc.date.available2020-08-05T07:03:47Z
dc.date.issued2020
dc.departmentİstanbul Medipol Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümü
dc.description.abstractHerbal medicine has been in use for centuries for a wide variety of ailments; however, the efficacy of its therapeutic agents in modern medicine is currently being studied. Curcuminoids are an example of natural agents, widely used due to their potential contribution in the prevention and treatment of cancer. In this study, the three main compounds of curcuminoids-curcumin, desmethoxycurcumin, and bisdesmethoxycurcumin-were determined by reversed-phase high performance liquid chromatography (HPLC) to quantify total content in a mixture. Subsequently, the effect of the three curcuminoids, employed as one sample, was evaluated, to study the proliferation, apoptosis, cell cycle, and migration of the human ovarian cancer cell line SKOV-3. The results reveal that curcuminoids inhibit the proliferation of SKOV-3 cells with concentration- and time-dependent mechanisms. The morphological analysis of the treated SKOV-3 cells showed a typical apoptotic phenotype-cell shrinkage and membrane blebbing in a dose-dependent manner. In addition, flow cytometry demonstrated an increase in apoptosis with an IC50 of 30 mu M curcuminoids. The migration of SKOV-3 cells was also inhibited, reflected by a decrease in wound area. Furthermore, the curcuminoids were found to have no stimulation effect on the expression of cytokines TNF-alpha and IL-10. These results suggest that a curcuminoid mixture can effectively suppress epithelial cancer cell growth in vitro by inducing cellular changes and apoptosis.
dc.description.sponsorshipDeanship of Scientific Research (DSR) at King Abdulaziz University (KAU), Jeddah, SAen_US
dc.identifier.citationAlmosa, H., Alqriqri, M., Denetiu, I., Baghdadi, M. A., Alkhaled, M., Alhosin, M. ... Üçışık, M. H. (2020). Cytotoxicity of standardized curcuminoids mixture against epithelial ovarian cancer cell line SKOV-3. Scientia Pharmaceutica, 88(1). https://dx.doi.org/10.3390/scipharm88010011
dc.identifier.doi10.3390/scipharm88010011
dc.identifier.issn2218-0532
dc.identifier.issue1
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://dx.doi.org/10.3390/scipharm88010011
dc.identifier.urihttps://hdl.handle.net/20.500.12511/5666
dc.identifier.volume88
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherMDPI
dc.relation.ispartofScientia Pharmaceuticaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsAttribution 4.0 International*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectCurcuminoids
dc.subjectOvarian Cancer
dc.subjectSKOV-3
dc.subjectFree Radical Scavengers
dc.subjectApoptosis
dc.subjectCell Cycle
dc.subjectCell Migration
dc.titleCytotoxicity of standardized curcuminoids mixture against epithelial ovarian cancer cell line SKOV-3
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Ucisik, Mehmet Hikmet-2020.pdf
Boyut:
3.96 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu