The association between different molecular weights of hyaluronic acid and CHAD, HIF-1 alpha, COL2A1 expression in chondrocyte cultures

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dc.authorid0000-0003-2003-6337
dc.contributor.authorYaşar Şirin, Duygu
dc.contributor.authorKaplan, Necati
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorÖzbek, Hanefi
dc.contributor.authorAkyuva, Yener
dc.contributor.authorKaya, Yasin Emre
dc.contributor.authorÖznam, Kadir
dc.contributor.authorAkkaya, Nuray
dc.contributor.authorGüler, Olcay
dc.contributor.authorAkkaya, Semih
dc.contributor.authorMahiroğulları, Mahir
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:50:40Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:50:40Z
dc.date.issued2018
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Farmakoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Ortopedi ve Travmatoloji Ana Bilim Dalı
dc.descriptionWOS: 000431850300018
dc.descriptionPubMed ID: 29849772
dc.description.abstractThe aim of the present study was to investigate the effects of three different formulations of hyaluronic acid (HA): Low molecular weight (MW) Sinovial One((R)), medium MW Viscoplus((R)) and high MW Durolane((R)), on chondrocyte proliferation and collagen type II (COL2A1), hypoxia-inducible factor 1 alpha (HIF-1 alpha) and chondroadherin (CHAD) expression in primary chondrocyte cultures. Standard primary chondrocyte cultures were established from osteochondral tissues surgically obtained from 6 patients with gonarthrosis. Cell morphology was evaluated using an inverted light microscope; cell proliferation was determined with a MTT assay and confirmed with acridine orange/propidium iodide staining. Levels of CHAD, COL2A1 and HIF-1 alpha expression were assessed using specific TaqMan gene expression assays. The results demonstrated the positive effect of HA treatment on cell proliferation, which was independent from the MW. COL2A1 expression increased in the medium and high MW HA treated groups. It was observed that HIF-1 alpha expression increased in the high MW treated group alone. CHAD expression increased only in the medium MW HA treated group. Evaluation of gene expression revealed that levels of expression increased as the duration of HA application increased, in the medium and high MW HA treated groups. In terms of increased viability and proliferation, a longer duration of HA application was more effective. Taken together, it may be concluded that the administration of medium and high MW HA may be a successful way of treating diseases affecting chondrocytes in a clinical setting.
dc.identifier.citationYaşar Şirin, D., Kaplan, N., Yılmaz, İ., Karaarslan, N., Özbek, H., Akyuva, Y. ... Mahiroğulları, M. (2018). The association between different molecular weights of hyaluronic acid and CHAD, HIF-1 alpha, COL2A1 expression in chondrocyte cultures. Experimental and Therapeutic Medicine, 15(5), 4205-4212. https://dx.doi.org/10.3892/etm.2018.5943
dc.identifier.doi10.3892/etm.2018.5943
dc.identifier.endpage4212
dc.identifier.issn1792-0981
dc.identifier.issn1792-1015
dc.identifier.issue5
dc.identifier.scopusqualityQ2
dc.identifier.startpage4205
dc.identifier.urihttps://dx.doi.org/10.3892/etm.2018.5943
dc.identifier.urihttps://hdl.handle.net/20.500.12511/2046
dc.identifier.volume15
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpandidos Publications Ltd
dc.relation.ispartofExperimental and Therapeutic Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectChondroadherin
dc.subjectCollagen Type II
dc.subjectHypoxia Inducible Factor 1 Alpha
dc.subjectHyaluronic Acid
dc.subjectChondrocytes
dc.titleThe association between different molecular weights of hyaluronic acid and CHAD, HIF-1 alpha, COL2A1 expression in chondrocyte cultures
dc.typeArticle

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