Exploiting stokes and anti-stokes type emission profiles of aptamer-functionalized luminescent nanoprobes for multiplex sensing applications

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dc.authorid0000-0002-1677-644X
dc.contributor.authorYüce, Meral
dc.contributor.authorKurt, Hasan
dc.contributor.authorHussain, Babar
dc.contributor.authorOw-Yang, Cleva W.
dc.contributor.authorBudak, Hikmet
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:50:30Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:50:30Z
dc.date.issued2018
dc.departmentİstanbul Medipol Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümü
dc.descriptionWOS: 000434412700016
dc.description.abstractMultiplex biosensing of four types of bacterial food pathogens in one pot is described. Stokes and anti-Stokes type photoluminescence (PL) of two quantum dots (QD) and two upconverting nanoparticles (UCNP) were utilised for realisation of the multiplex detection. The biosensing system was composed of aptamer-functionalized QD and UCNP nanoprobes that were conjugated with partially complementary DNA-modified magnetic beads for separation. PL signals of the conjugates were collected before and after incubation with target pathogens in one pot through the sequential excitations at 335nm for QD probes and 980nm for UCNP probes. The limit of detection values achieved were 28, 15, 12 and 25 cfu center dot mL(-1) for Listeria monocytogenes, Staphylococcus aureus, Salmonella typhimurium and Pseudomonas aeruginosa, respectively. Efforts for extending the multiplex detection up to five pathogens were also presented even though the PL signal cross-talk of a third QD nanoprobe hindered the detection. This work empowers co-deployment of QDs and UCNPs and paves the way for future studies in multiplex sensing, labelling, and bioimaging fields.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [114Z379]; TUBITAK [2215]en_US
dc.description.sponsorshipThe authors acknowledge the Scientific and Technological Research Council of Turkey (TUBITAK), Grant ID: 114Z379 for the financial support. B.H. acknowledges TUBITAK 2215 Graduate Scholarship Program. The authors would like to thank PhD candidates, Tug. dem Muslu (Sabanci University) and Kadriye Kahraman (Sabanci University) for maintenance of the bacterial cultures and Assist. Prof. Hayriye Unal (Sabanci University) for providing access to the PMT-integrated fluorescence spectrometer. M.Y. owes special thanks to Assist. Prof. Tolga Sutlu (Sabanci University) for the administrative support.en_US
dc.identifier.citationYüce, M., Kurt, H., Hussain, B., Ow-Yang, C. ve Budak, H. (2018). Exploiting stokes and anti-stokes type emission profiles of aptamer-functionalized luminescent nanoprobes for multiplex sensing applications. Chemistryselect, 3(21), 5814-5823. https://dx.doi.org/10.1002/slct.201801008
dc.identifier.doi10.1002/slct.201801008
dc.identifier.endpage5823
dc.identifier.issn2365-6549
dc.identifier.issue21
dc.identifier.scopusqualityQ2
dc.identifier.startpage5814
dc.identifier.urihttps://dx.doi.org/10.1002/slct.201801008
dc.identifier.urihttps://hdl.handle.net/20.500.12511/2000
dc.identifier.volume3
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley-VCH Verlag Gmbh
dc.relation.ecinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/114Z379
dc.relation.ecinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/2215
dc.relation.ispartofChemistryselecten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectAptamers
dc.subjectBiosensor
dc.subjectFoodborne Pathogens
dc.subjectQuantum Dots
dc.subjectUpconverting Nanoparticles
dc.titleExploiting stokes and anti-stokes type emission profiles of aptamer-functionalized luminescent nanoprobes for multiplex sensing applications
dc.typeArticle

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