Probing subcellular iron availability with genetically encoded nitric oxide biosensors

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dc.authorid0000-0002-9373-0808
dc.contributor.authorSevimli, Gülşah
dc.contributor.authorAlston, Amy E.
dc.contributor.authorFunk, Felix
dc.contributor.authorFlühmann, Beat
dc.contributor.authorMalli, Roland
dc.contributor.authorGraier, Wolfgang F.
dc.contributor.authorEroğlu, Emrah
dc.date.accessioned2022-11-04T08:44:23Z
dc.date.available2022-11-04T08:44:23Z
dc.date.issued2022
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.description.abstractCellular iron supply is required for various biochemical processes. Measuring bioavailable iron in cells aids in obtaining a better understanding of its biochemical activities but is technically challenging. Existing techniques have several constraints that make precise localization difficult, and the lack of a functional readout makes it unclear whether the tested labile iron is available for metalloproteins. Here, we use geNOps; a ferrous iron-dependent genetically encoded fluorescent nitric oxide (NO) biosensor, to measure available iron in cellular locales. We exploited the nitrosylation-dependent fluorescence quenching of geNOps as a direct readout for cellular iron absorption, distribution, and availability. Our findings show that, in addition to ferrous iron salts, the complex of iron (III) with N,N’-bis (2-hydroxybenzyl)ethylenediamine-N,N’-diacetic acid (HBED) can activate the iron (II)-dependent NO probe within intact cells. Cell treatment for only 20 min with iron sucrose was also sufficient to activate the biosensor in the cytosol and mitochondria significantly; however, ferric carboxymaltose failed to functionalize the probe, even after 2 h of cell treatment. Our findings show that the geNOps approach detects available iron (II) in cultured cells and can be applied to assay functional iron (II) at the (sub)cellular level.
dc.description.sponsorshipVifor Pharmaen_US
dc.identifier.citationSevimli, G., Alston, A. E., Funk, F., Flühmann, B., Malli, R., Graier, W. F. ... Eroğlu, E. (2022). Probing subcellular iron availability with genetically encoded nitric oxide biosensors. Biosensors-Basel, 12(10). https://doi.org/10.3390/bios12100903
dc.identifier.doi10.3390/bios12100903
dc.identifier.issn2079-6374
dc.identifier.issue10
dc.identifier.pmid36291039
dc.identifier.scopus2-s2.0-85140360994
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.3390/bios12100903
dc.identifier.urihttps://hdl.handle.net/20.500.12511/9923
dc.identifier.volume12
dc.identifier.wos000872288800001en_US
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorEroğlu, Emrah
dc.language.isoen
dc.publisherMDPI (Multidisipliner Digital Publishing Institute)
dc.relation.ispartofBiosensors-Baselen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsAttribution 4.0 International*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectCarboxymaltose
dc.subjectCellular İron Uptake
dc.subjectFerinject
dc.subjectFluorescent Biosensor
dc.subjectgeNOps
dc.subjectHBED
dc.subjectİron Sucrose
dc.subjectLabile İron
dc.subjectVenofer
dc.titleProbing subcellular iron availability with genetically encoded nitric oxide biosensors
dc.typeArticle

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