Dose-dependent effect of a new biotin compound in hippocampal remyelination in rats

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dc.contributor.authorYuluğ, Burak
dc.contributor.authorKılıç, Ertuğrul
dc.contributor.authorOğuz, Tuba
dc.contributor.authorOrhan, Cemal
dc.contributor.authorEr, Beşir
dc.contributor.authorTuzcu, Mehmet
dc.contributor.authorŞahin, Kazım
dc.date.accessioned2026-03-18T12:31:40Z
dc.date.available2026-03-18T12:31:40Z
dc.date.issued2025
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.departmentİstanbul Medipol Üniversitesi, Sağlık Bilimleri Enstitüsü, Sinirbilim Ana Bilim Dalı
dc.description.abstractDemyelination is commonly observed in neurodegenerative disorders, including multiple sclerosis (MS). Biotin supplementation is known to stabilize MS progression. To reduce the effective dose of biotin, we synthesized a new and superior form of biotin, a complex of magnesium ionically bound to biotin (MgB) and compared its dose-dependent effect with biotin alone after inducing demyelination using lysolecithin (LPC) in rats. Myelination was assessed using luxol fast blue staining and immunostaining against MBP protein, revealing that the most significant remyelination occurred in the MgB groups. Additionally, both biotin and MgB-treated animals showed dose-dependent improvements in spatial memory. Moreover, we detected a decrease in inflammatory proteins in both treatment groups, which was more prominent in high-dose MgB-treated animals and correlated with decreased expression of NF-kappa B p65, OP, and MMP-9 proteins. Further analysis of biotin-related proteins demonstrated that both biotin and, notably, MgB reversed the demyelination-dependent reduction of these proteins. Furthermore, biotin, particularly MgB, improved neuronal transmission proteins, Synapsin-1, PSD-93, and PSD-95. Additionally, both treatment groups exhibited increased BDNF, GAP43, and ICAM levels, with significant increments observed in high-dose MgB-treated animals. Increased GFAP, indicative of reactive gliosis, was observed in LPC-treated animals, and this effect was notably reversed by high-dose MgB treatment. The current data emphasize the dose-dependent beneficial effect on the remyelination process. Furthermore, the combination of biotin with Mg resulted in a more potent effect compared to biotin by itself. The strong influence of MgB encourages proof-of-concept studies using MgB in patients with MS.
dc.description.sponsorshipJDS Therapeutics, LLC (Purchase, NY, USA) ; Turkish Academy of Sciences
dc.identifier.citationYuluğ, B., Kılıç, E., Oğuz, T., Orhan, C., Er, B., Tuzcu, M. ... Şahin, K. (2025). Dose-dependent effect of a new biotin compound in hippocampal remyelination in rats. Molecular Neurobiology, 62(5), 6503-6520. http://dx.doi.org/10.1007/s12035-025-04686-y
dc.identifier.doi10.1007/s12035-025-04686-y
dc.identifier.endpage6520
dc.identifier.issn0893-7648
dc.identifier.issn1559-1182
dc.identifier.issue5
dc.identifier.pmid39821844
dc.identifier.scopus2-s2.0-85217234654
dc.identifier.scopusqualityQ1
dc.identifier.startpage6503
dc.identifier.urihttp://dx.doi.org/10.1007/s12035-025-04686-y
dc.identifier.urihttps://hdl.handle.net/20.500.12511/13405
dc.identifier.volume62
dc.identifier.wosWOS:001398676000001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKılıç, Ertuğrul
dc.institutionauthorOğuz, Tuba
dc.institutionauthorid0000-0001-6494-8923
dc.language.isoen
dc.relation.ispartofMolecular Neurobiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBiotin
dc.subjectDemyelination
dc.subjectMagnesium-Biotin
dc.subjectMultiple Sclerosis
dc.subjectRemyelination
dc.titleDose-dependent effect of a new biotin compound in hippocampal remyelination in rats
dc.typeArticle

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