Predicting the CD34 content of mobilized peripheral blood leukapheresis products: Single institution experience over 20 years

Basit Öğe Kaydı
dc.authorid0000-0003-1508-2822
dc.contributor.authorMutlu, Yaşa Gül
dc.contributor.authorSevcik, Joan
dc.contributor.authorKiss, Joseph Elmer
dc.contributor.authorLister, John
dc.contributor.authorMoore, Linda R.
dc.contributor.authorDonnenberg, Albert D.
dc.date.accessioned2024-03-11T11:03:16Z
dc.date.available2024-03-11T11:03:16Z
dc.date.issued2024
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, İç Hastalıkları Ana Bilim Dalı
dc.description.abstractBackground aims: Since the standardization of CD34 measurement by flow cytometry, predictors of leukapheresis CD34 yield have played a pivotal role in planning donor leukaphereses. We describe here a single institution's experience with a multivariate predictor that was used for 2,929 products without alteration for 20 years. Methods: The ordinary least squares regression model variables included log peripheral CD34 count, collection duration (3- versus 4 -hours), collection number, donor sex, and transplant type. Results: During the study period we changed flow cytometers twice and leukapheresis instruments once. During the Cobe Spectra era the predictor explained 90% of the variability in CD34 collection yield for autologous transplants (r(2) = 0.90), and 70% for allogeneic transplants with an overall sensitivity to predict a CD34 yield of >= 1 x 10(6)/kg of 97.7%, and specificity of 81.4%. Conclusions: Implemented prospectively with real-time result reporting, the model allowed us to predict CD34 yield with both 3- and 4 -hour collection scenarios. Given this guidance, 3 -hour collections were selected by the clinical team 25% of the time, saving patient leukapheresis time and resources. When faced with a prediction of <1 x 10(6) CD34/kg, the clinical team chose to defer collection 72% of the time. In instances where leukapheresis was performed despite a poor predicted outcome, 85% of patients collected on the Cobe Spectra, and 92% of patients collected on the Optia, failed to collect at least 1 x 10(6) CD34/kg. A revised model is tested retrospectively on Optia data, and suggestions for further improvements are discussed.
dc.identifier.citationMutlu, Y. G. Sevcik, J., Kiss, J. E., Lister, J., Moore, L. R. ve Donnenberg, A. D. (2024). Predicting the CD34 content of mobilized peripheral blood leukapheresis products: Single institution experience over 20 years. Cytotherapy, 26(2), 171-177. https://dx.doi.org/10.1016/j.jcyt.2023.09.005
dc.identifier.doi10.1016/j.jcyt.2023.09.005
dc.identifier.endpage177
dc.identifier.issn1465-3249
dc.identifier.issn1477-2566
dc.identifier.issue2
dc.identifier.pmid37930293
dc.identifier.startpage171
dc.identifier.urihttps://dx.doi.org/10.1016/j.jcyt.2023.09.005
dc.identifier.urihttps://hdl.handle.net/20.500.12511/12354
dc.identifier.volume26
dc.identifier.wos001170787300001en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.institutionauthorMutlu, Yaşa Gül
dc.language.isoen
dc.publisherElsevier Science Ltd
dc.relation.ispartofCytotherapyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectCD34
dc.subjectFlow Cytometry
dc.subjectLeukapheresis
dc.subjectMobilization
dc.subjectPredictor
dc.titlePredicting the CD34 content of mobilized peripheral blood leukapheresis products: Single institution experience over 20 years
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
Mutlu-Yasa-2024.pdf
Boyut:
1.48 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu