Calculation of the Ki67 index in pancreatic neuroendocrine tumors: A comparative analysis of four counting methodologies

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dc.authorid0000-0001-6830-7701
dc.contributor.authorReid, Michelle Dian
dc.contributor.authorBağcı, Pelin
dc.contributor.authorOhike, Nobuyuki
dc.contributor.authorSaka, Burcu
dc.contributor.authorErbarut Seven, İpek
dc.contributor.authorDursun, Nevra
dc.contributor.authorBalcı, Serdar
dc.contributor.authorGucer, Hasan
dc.contributor.authorJang, Kee-Taek
dc.contributor.authorTajiri, Takuma
dc.contributor.authorBaştürk, Olca
dc.contributor.authorKong, So Yeon
dc.contributor.authorGoodman, Michael
dc.contributor.authorAkkaş, Gizem
dc.contributor.authorAdsay, Volkan
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:01:48Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:01:48Z
dc.date.issued2015
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Tıbbi Patoloji Ana Bilim Dalı
dc.descriptionWOS: 000353774200008
dc.descriptionPubMed ID: 25412850
dc.description.abstractKi67 index is now an essential part of classification of pancreatic neuroendocrine tumors. However, its adaptation into daily practice has been fraught with challenges related to counting methodology. In this study, three reviewers used four counting methodologies to calculate Ki67 index in 68 well-differentiated pancreatic neuroendocrine tumors: (1) 'eye-ball' estimation, which has been advocated as reliable and is widely used; (2) automated counting by image analyzer; (3) manual eye-counting (eye under a microscope without a grid); and (4) manual count of camera-captured/printed image. Pearson's correlation (R) was used to measure pair-wise correlation among three reviewers using all four methodologies. Average level of agreement was calculated using mean of R values. The results showed that: (1) 'eye-balling' was least expensive and fastest (average time <1 min) but had poor reliability and reproducibility. (2) Automated count was the most expensive and least practical with major impact on turnaround time (limited by machine and personnel accessibility), and, more importantly, had inaccuracies in overcounting unwanted material. (3) Manual eye count had no additional cost, averaged 6 min, but proved impractical and poorly reproducible. (4) Camera-captured/printed image was most reliable, had highest reproducibility, but took longer than 'eye-balling'. In conclusion, based on its comparatively low cost/benefit ratio and reproducibility, camera-captured/printed image appears to be the most practical for calculating Ki67 index. Although automated counting is generally advertised as the gold standard for index calculation, in this study it was not as accurate or cost-effective as camera-captured/printed image and was highly operator-dependent. 'Eye-balling' produces highly inaccurate and unreliable results, and is not recommended for routine use.
dc.description.sponsorshipTurkiye Bilimsel ve Teknik Arastirma Kurumu (State Department of Scientific and Technical Investigation) of the Republic of Turkeyen_US
dc.description.sponsorshipA preliminary analysis involving part of this cohort was reported at the 101st annual meeting (in 2012) of the United States and Canadian Academy of Pathology in Vancouver, Canada. Dr Nevra Dursun is the recipient of a scholarship from Turkiye Bilimsel ve Teknik Arastirma Kurumu (State Department of Scientific and Technical Investigation) of the Republic of Turkey. The other authors declare no conflict of interest.en_US
dc.identifier.citationReid, M., Bağcı, P., Ohike, N., Saka, B., Erbarut Seven, İ., Dursun, N. ... Adsay, V. (2015). Calculation of the Ki67 index in pancreatic neuroendocrine tumors: A comparative analysis of four counting methodologies. Modern Pathology, 28(5), 686-694. https://dx.doi.org/10.1038/modpathol.2014.156
dc.identifier.doi10.1038/modpathol.2014.156
dc.identifier.endpage694
dc.identifier.issn0893-3952
dc.identifier.issn1530-0285
dc.identifier.issue5
dc.identifier.scopusqualityQ1
dc.identifier.startpage686
dc.identifier.urihttps://dx.doi.org/10.1038/modpathol.2014.156
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3450
dc.identifier.volume28
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherNature Publishing Group
dc.relation.ispartofModern Pathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectKi67 Index
dc.subjectHowever
dc.subjectEye-balling
dc.titleCalculation of the Ki67 index in pancreatic neuroendocrine tumors: A comparative analysis of four counting methodologies
dc.typeArticle

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