Comparison of biomicroscopy and light microscopy findings in demodex diagnosis in patients with chronic blepharitis

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dc.authorid0000-0003-2445-6339
dc.authorid0000-0001-6090-4448
dc.authorid0000-0002-5079-4713
dc.authorid0000-0002-6408-2709
dc.authorid0000-0001-8954-5055
dc.authorid0000-0001-8893-1739
dc.contributor.authorTanrıverdi, Cafer
dc.contributor.authorBalcı, Özlem
dc.contributor.authorDemirci, Göktuğ
dc.contributor.authorOdabaşı, Mahmut
dc.contributor.authorÖzsütçü, Mustafa
dc.contributor.authorNurözler Tabakçı, Burcu
dc.date.accessioned2020-03-05T09:42:06Z
dc.date.available2020-03-05T09:42:06Z
dc.date.issued2020
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Göz Hastalıkları Ana Bilim Dalı
dc.description.abstractOBJECTIVES: We aimed to compare the results of biomicroscopic examination and light microscopy in the diagnosis of Demodex infestation in chronic blepharitis cases. METHODS: The study was performed with retrospective data and included patients with chronic blepharitis and who were admitted to the Medipol University Ophthalmology Outpatient Clinic. Biomicroscopic evaluation was performed intentionally under 40x magnification to investigate the presence of Demodex ectoparasites. Three or four pieces of eyelashes were obtained from all cases with epilation technique, and Demodex parasites were examined under ×100 and ×400 magnifications with light microscopy. The presence of one or more Demodex parasites in the examined area under light microscopy was considered to be positive for infestation. We calculated sensitivity, specificity, false-positive rate, false-negative rate, and positive and negative predictive values of biomicroscopic examination compared with those of positive light microscopy. RESULTS: Of the 255 subjects included in the study, 134 (52.5%) were men and 121 (47.5%) were women. The mean age was 43.9±11.9 years. Of the 255 chronic blepharitis cases, 130 (51%) presented Demodex infestation on light microscopy. We calculated the sensitivity, specificity, false-positive rate, false-negative rate, positive predictive value, and negative predictive value and were found to be 83.07%, 90.4%, 9.6%, 16.9%, 90%, and 83.7%, respectively, for the biomicroscopic examination. CONCLUSIONS: Careful biomicroscopic examination can be time-efficient and cost-effective, and the need for more advanced and invasive procedures for the identification of Demodex in patients with chronic blepharitis can be reduced.
dc.identifier.citationTanrıverdi, C., Balcı, Ö., Demirci, G., Odabaşı, M., Özsütçü, M. ve Nurözler Tabakçı, B. (2020). Comparison of biomicroscopy and light microscopy findings in demodex diagnosis in patients with chronic blepharitis. Eye and Contact Lens: Science and Clinical Practice, 46(5), 297-300. http://doi.org/10.1097/ICL.0000000000000660
dc.identifier.doi10.1097/ICL.0000000000000660
dc.identifier.endpage300
dc.identifier.issue5
dc.identifier.scopusqualityN/A
dc.identifier.startpage297
dc.identifier.urihttp://doi.org/10.1097/ICL.0000000000000660
dc.identifier.urihttps://hdl.handle.net/20.500.12511/4999
dc.identifier.volume46
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWolters Kluwer
dc.relation.ispartofEye and Contact Lens: Science and Clinical Practiceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectBlepharitis
dc.subjectDemodex
dc.subjectDemodex Diagnosis
dc.subjectBiomicroscopy
dc.subjectLight Microscopy
dc.titleComparison of biomicroscopy and light microscopy findings in demodex diagnosis in patients with chronic blepharitis
dc.typeArticle

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