Experimentally induced metamorphosis in highly regenerative axolotl (ambystoma mexicanum) under constant diet restructures microbiota

Basit Öğe Kaydı
dc.authorid0000-0002-1502-1600
dc.authorid0000-0002-4479-2586
dc.authorid0000-0002-7059-1884
dc.authorid0000-0003-0352-1947
dc.authorid0000-0002-2752-1223
dc.contributor.authorDemircan, Turan
dc.contributor.authorOvezmyradov, Guvanch
dc.contributor.authorYıldırım, Berna
dc.contributor.authorKeskin, İlknur
dc.contributor.authorİlhan, Ayşe Elif
dc.contributor.authorFesçioğlu, Ece Cana
dc.contributor.authorÖztürk, Gürkan
dc.contributor.authorYıldırım, Süleyman
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:50:25Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:50:25Z
dc.date.issued2018
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Biyoistatistik ve Tıp Bilişimi Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.descriptionWOS: 000439278300009
dc.descriptionPubMed ID: 30030457
dc.description.abstractAxolotl (Ambystoma mexicanum) is a critically endangered salamander species and a model organism for regenerative and developmental biology. Despite life-long neoteny in nature and in captive-bred colonies, metamorphosis of these animals can be experimentally induced by administering Thyroid hormones (THs). However, microbiological consequences of this experimental procedure, such as host microbiota response, remain largely unknown. Here, we systematically compared host bacterial microbiota associated with skin, stomach, gut tissues and fecal samples, between neotenic and metamorphic axolotls based on 16S rRNA gene sequences. Our results show that distinct bacterial communities inhabit individual organs of axolotl and undergo substantial restructuring through metamorphosis. Skin microbiota among others, shifted sharply, as highlighted by a major transition from Firmicutes-enriched to Proteobacteria-enriched relative abundance and precipitously decreased diversity. Fecal microbiota of neotenic and metamorphic axolotl shared relatively higher similarity, suggesting that diet continues to shape microbiota despite fundamental transformations in the host digestive organs. We also reproduced the previous finding on reduction in regenerative capacity in limbs of axolotl following metamorphosis, highlighting the need to investigate whether shifts in microbiota is causally linked to regenerative capacity of axolotl. The initial results on axolotl microbiota provide novel insights into microbiological aspects of axolotl metamorphosis and will establish a baseline for future in-depth studies.
dc.description.sponsorshipMedipol University Research Funden_US
dc.description.sponsorshipThis study was financially supported by the Medipol University Research Fund. This study used the Nephele platform from the National Institute of Allergy and Infectious Diseases (NIAID) Office of Cyber Infrastructure and Computational Biology (OCICB) in Bethesda, MD.en_US
dc.identifier.citationDemircan, T., Ovezmyradov, G., Yıldırım, B., Keskin, İ., İlhan, A., Fesçioğlu, E. ... Yıldırım, S. (2018). Experimentally induced metamorphosis in highly regenerative axolotl (ambystoma mexicanum) under constant diet restructures microbiota. Scientific Reports, 8. https://dx.doi.org/10.1038/s41598-018-29373-y
dc.identifier.doi10.1038/s41598-018-29373-y
dc.identifier.issn2045-2322
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://dx.doi.org/10.1038/s41598-018-29373-y
dc.identifier.urihttps://hdl.handle.net/20.500.12511/1980
dc.identifier.volume8
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherNature Publishing Group
dc.relation.ispartofScientific Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsAttribution 4.0 International*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectAmbystoma Mexicanum
dc.subjectConstant Diet
dc.subjectRegenerative Axolotl
dc.subjectMicrobiota
dc.titleExperimentally induced metamorphosis in highly regenerative axolotl (ambystoma mexicanum) under constant diet restructures microbiota
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Demircan, T..pdf
Boyut:
2.08 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
Makale Koleksiyonu
Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu