mTor Is a signaling hub in cell survival: A mass-spectrometry-based proteomics investigation

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dc.authorid0000-0002-8814-7351
dc.contributor.authorTang, Zhi
dc.contributor.authorBaykal, Ahmet Tarık
dc.contributor.authorGao, Hui
dc.contributor.authorQuezada, Hernan Concha
dc.contributor.authorZhang, Haiyan
dc.contributor.authorBereczki, Erika
dc.contributor.authorSerhatlı, Müge
dc.contributor.authorBaykal, Betül
dc.contributor.authorAcıoğlu, Çiğdem
dc.contributor.authorWang, Shan
dc.contributor.authorIoja, Eniko
dc.contributor.authorJi, Xinying
dc.contributor.authorZhang, Yan
dc.contributor.authorGuan, Zhizhong
dc.contributor.authorWinblad, Bengt
dc.contributor.authorPei, Jin-Jing
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:01:29Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:01:29Z
dc.date.issued2014
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.descriptionWOS: 000335490600018
dc.descriptionPubMed ID: 24694195
dc.description.abstractmTor plays a central role in controlling protein homeostasis and cell survival. Recently, we have demonstrated that perturbations of mTor signaling are implicated in Alzheimer's disease (AD) and that mTor complex 1 (mTorC1) is involved in the formation of toxic phospho-tau. Therefore, we employed mass-spectrometry-based proteomics to identify specific protein expression changes in relation with cell survival in human neuroblastoma SH-SYSY cells expressing genetically modified mTor. Cell death in SH-SYSY cells was induced by moderate serum deprivation. Using flow cytometry we observed that up-regulated mTor complex 2 (mTorC2) increases the number of viable cells. By using a combination approach of proteomic and enrichment analysis we have identified several proteins (Thioredoxin-dependent peroxide reductase, Peroxiredoxin-5, Cofilin 1 (non-muscle), Annexin A5, Mortalin, and 14-3-3 protein zeta/delta) involved in mitochondrial integrity, apoptotosis, and pro-survival functions (caspase inhibitor activity and anti-apoptosis) that were significantly altered by mTor activity modulation. The major findings of this study are the implication of mTorC2 but not mTorC1 in cell viability modulation by activating the pro-survival machinery. Taken together, these results suggest that up-regulated mTorC2 might be playing an important role in promoting cell survival by suppressing the mitochondria-caspase-apoptotic pathway in vitro.
dc.description.sponsorshipKarolinska Institutet Research fundsen_US
dc.description.sponsorshipDementia Foundationen_US
dc.description.sponsorshipAlzheimerfondenen_US
dc.description.sponsorshipWallenberg Foundationen_US
dc.description.sponsorshipGun and Bertil Stohne Foundationen_US
dc.description.sponsorshipGamla Tjanarinnor Foundationen_US
dc.description.sponsorshipThis work was supported by Karolinska Institutet Research funds, the Dementia Foundation, Alzheimerfonden, Wallenberg Foundation, Gun and Bertil Stohne Foundation, and Gamla Tjanarinnor Foundation.en_US
dc.identifier.citationTang, Z., Baykal, A. T., Gao, H., Quezada, H. C., Zhang, H., Bereczki, E. ... Pei, J.-J. (2014). mTor Is a signaling hub in cell survival: A mass-spectrometry-based proteomics investigation. Journal of Proteome Research, 13(5), 2433-2444. https://dx.doi.org/10.1021/pr500192g
dc.identifier.doi10.1021/pr500192g
dc.identifier.endpage2444
dc.identifier.issn1535-3893
dc.identifier.issn1535-3907
dc.identifier.issue5
dc.identifier.scopusqualityQ1
dc.identifier.startpage2433
dc.identifier.urihttps://dx.doi.org/10.1021/pr500192g
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3301
dc.identifier.volume13
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.relation.ispartofJournal of Proteome Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectmTor
dc.subjectCell Survival
dc.subjectApoptosis
dc.subjectCaspases
dc.subjectMitochondria
dc.titlemTor Is a signaling hub in cell survival: A mass-spectrometry-based proteomics investigation
dc.typeArticle

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