Wedge prism assisted quantitative phase imaging on standard microscopes

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dc.contributor.authorToy, Mehmet Fatih
dc.date.accessioned2019-12-19T11:05:55Z
dc.date.available2019-12-19T11:05:55Z
dc.date.issued2019
dc.departmentİstanbul Medipol Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümü
dc.description.abstractAdoption of quantitative phase imaging for biomicroscopy applications has been limited due to the technical knowledge requirements or the cost of commercial systems. Here, a wedge prism based simple approach is introduced to acquire quantitative phase images on a standard microscope. The proposed approach minimizes the alignment requirements and the cost. Acquired quantitative phase images reflect the sample morphology and the refractive index. Hence, it enables quantitative microscopy without the need for labeling. Optimization parameters are explored for the physical constraints of the cameras and microscope objectives. Experimental quantitative phase images are provided for the USAF-1951 test target, epithelial cell samples, and microsphere samples. Cell imaging results demonstrate the qualitative performance in an exemplary scenario. Quantitative phase imaging of microsphere samples provides experimental proof for the preservation of the resolving power.
dc.description.sponsorshipIstanbul Development Agency (ISTKA), Innovative and Creative Istanbul Financial Support Program 2016en_US
dc.identifier.citationToy, M. F. (2019). Wedge prism assisted quantitative phase imaging on standard microscopes. Optics Communications, 451, 361-366. https://doi.org/10.1016/j.optcom.2019.07.010
dc.identifier.doi10.1016/j.optcom.2019.07.010
dc.identifier.endpage366
dc.identifier.issn0030-4018
dc.identifier.issn1873-0310
dc.identifier.issue451
dc.identifier.scopusqualityQ1
dc.identifier.startpage361
dc.identifier.urihttps://doi.org/10.1016/j.optcom.2019.07.010
dc.identifier.urihttps://hdl.handle.net/20.500.12511/4547
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofOptics Communicationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectMicroscopy
dc.subjectPhase Imaging
dc.subjectHolography
dc.titleWedge prism assisted quantitative phase imaging on standard microscopes
dc.typeArticle

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