Biosynthesis of zinc oxide nanoparticles using dried leaves of camellia sinensis: methods to characterize and assess their effects on mesenchymal stem cell viability

dc.contributor.authorYeşilkır Baydar, Serap
dc.contributor.authorAkgül Çağlar, Tuba
dc.contributor.authorKoç, Fatma Ebru
dc.date.accessioned2026-04-09T11:05:56Z
dc.date.available2026-04-09T11:05:56Z
dc.date.issued2025
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.description.abstractStem cell nanotechnology (SCN) is an important scientific field to guide stem cell-based research of nanoparticles. Currently, nanoparticles (NPs) have a rich spectrum regarding the sources from which they are obtained (metallic, polymeric, etc.), the methods of obtaining them (physical, chemical, biological), and their shape, size, electrical charge, etc. properties. It is also essential to expand green synthesis applications for the use of NPs in the field of biomedical sciences. For this purpose, there is a need to produce NPs using biological sources (plant, microorganism, algae, yeast etc.…), characterization and investigation of their effects on biological activities of stem cells. This process involves long and laborious procedures, and there may be differences in methods between individual laboratories.In this protocol, biofabrication and characterization of ZnO NPs using dried leaves of Camellia sinensis is described. This experimental setup includes conventional and novel methods that can be applied to biofabricate and characterize the NPs and to examine the viability, apoptotic, and necrotic effects on human adipose tissue-derived mesenchymal stem cells (ADMSCs) in vitro.
dc.identifier.citationYeşilkır Baydar, S., Akgül Çağlar, T. ve Koç, F. E. (2025). Biosynthesis of zinc oxide nanoparticles using dried leaves of camellia sinensis: methods to characterize and assess their effects on mesenchymal stem cell viability. Methods in Molecular Biology. http://dx.doi.org/10.1007/7651_2024_598
dc.identifier.doi10.1007/7651_2024_598
dc.identifier.pmid39821805
dc.identifier.urihttps://hdl.handle.net/20.500.12511/13406
dc.indekslendigikaynakPubMed
dc.institutionauthorAkgül Çağlar, Tuba
dc.institutionauthorid0000-0003-2033-860X
dc.language.isoen
dc.relation.ispartofMethods in Molecular Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBiofabrication
dc.subjectCamellia Sinensis
dc.subjectCell Viability
dc.subjectGreen Nanotechnology
dc.subjectStem Cell Nanotechnology
dc.titleBiosynthesis of zinc oxide nanoparticles using dried leaves of camellia sinensis: methods to characterize and assess their effects on mesenchymal stem cell viability
dc.typeArticle

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