The creation of unilateral intermittent and unintermittent renal ischemia-reperfusion models in rats

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dc.authorid0000-0002-0159-2090
dc.authorid0000-0002-8662-5284
dc.authorid0000-0002-7059-1884
dc.authorid0000-0002-0675-1839
dc.authorid0000-0002-5009-8435
dc.authorid0000-0001-8014-2321
dc.authorid0000-0002-4245-7506
dc.contributor.authorSoytaş, Mustafa
dc.contributor.authorGürsoy, Duygu
dc.contributor.authorBoz, Mustafa Yücel
dc.contributor.authorÇakıcı, Çağrı
dc.contributor.authorKeskin, İlknur
dc.contributor.authorYiğitbaşı, Türkan
dc.contributor.authorGüven, Selçuk
dc.contributor.authorHoruz, Rahim
dc.contributor.authorAlbayrak, Selami
dc.date.accessioned2021-11-05T06:53:58Z
dc.date.available2021-11-05T06:53:58Z
dc.date.issued2021
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Üroloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.description.abstractBackground and Aim: This study aims to establish unilateral intermittent and unintermittent partial nephrectomy-like renal ischemia-reperfusion (I-R) model in rats and to compare the results with biochemical findings. Material and Methods: The study was conducted on 24 adult 8-week-old male Wistar-Albino rats, each weighing s200-250 g. The rats were divided into three groups. In the Sham group (n = 8), the kidney was surgically exposed and closed. We designed experimental I-R models in the second group (n = 8, a total of 30-min ischemia model in the manner of 3 intermittent sets 8 minutes clamping and 2 min unclamping) and in the third group (n = 8, one session of 30-min unintermittent ischemia). In postoperative day 1, the rats were sacrificed, and the effects of I-R models on the renal tissue were comparatively assessed by evaluating serum Neutrophil Gelatinase-Associated Lipocalin (NGAL), serum kidney injury molecule-1 (KIM-1), urinary NGAL, urinary KIM-1, and serum creatinine levels. Results: Urinary NGAL and KIM-1 levels were significantly higher in the continuous ischemia group when compared to those in the sham and intermittent ischemia groups (P < 0.05). In the intermittent ischemia group, urinary NGAL and urinary KIM-1 levels were significantly higher than those in the sham group (P < 0.05). Although the results of serum NGAL, serum KIM-1, and serum creatinine levels seemed to be in parallel to the results of urinary markers, no statistically significant difference was found. Conclusion: Renal injury was significantly less in the intermittent I-R model when compared to that in the unintermittent I-R model in our experimental rat study.
dc.identifier.citationSoytaş, M., Gürsoy, D., Boz, M. Y., Çakıcı, Ç., Keskin, İ., Yiğitbaşı, T. ... Albayrak, S. (2021). The creation of unilateral intermittent and unintermittent renal ischemia-reperfusion models in rats. Urology Annals, 13(4), 378-383. https://dx.doi.org/10.4103/UA.UA_79_20
dc.identifier.doi10.4103/UA.UA_79_20
dc.identifier.endpage383
dc.identifier.issn0974-7796
dc.identifier.issn0974-7834
dc.identifier.issue4
dc.identifier.scopusqualityQ3
dc.identifier.startpage378
dc.identifier.urihttps://dx.doi.org/10.4103/UA.UA_79_20
dc.identifier.urihttps://hdl.handle.net/20.500.12511/8566
dc.identifier.volume13
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWolters Kluwer Medknow Publications
dc.relation.ispartofUrology Annalsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectIntermittent Ischemia-Reperfusion
dc.subjectIschemia-Reperfusion Injury
dc.subjectPartial Nephrectomy
dc.subjectRats
dc.subjectRenal Failure
dc.titleThe creation of unilateral intermittent and unintermittent renal ischemia-reperfusion models in rats
dc.typeArticle

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