Virulence factor of porphyromonas gingivalis disrupts intestinal oxidative status and acetylcholinesterase activity in rotenone-exposed zebrafish

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dc.contributor.authorİşlek, İpek
dc.contributor.authorBeler, Merih
dc.contributor.authorÜnal, İsmail
dc.contributor.authorCansız, Derya
dc.contributor.authorEmekli Alturfan, Ebru
dc.contributor.authorKöse, Kemal Naci
dc.date.accessioned2026-01-09T10:40:28Z
dc.date.available2026-01-09T10:40:28Z
dc.date.issued2024
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.description.abstractObjective: Porphyromonas gingivalis (P. gingivalis) is a major pathogenic bacterium in periodontal disease and is associated with neurodegenerative diseases. One of the most destructive endotoxins of P. gingivalis is gingipain. Our objective was to show the impact of gingipain on acetylcholinesterase (AChE) activity and oxidative status in the intestinal tissues of zebrafish exposed to rotenone. Materials and Methods: Zebrafish were grouped as; control group (C), gingipain-injected group (G), rotenone-exposed group (R), gingipain-injected and rotenone-exposed group (G+R) (n=15). At the end of 4 weeks, spectrophotometric analyses were performed to evaluate the oxidative status and AChE activity in the intestinal tissues. Results: Intestinal lipid peroxidation (LPO) levels were higher in the G group than in the C group. Gingipain injection significantly reduced the activities of AChE and superoxide dismutase (SOD). In the R group, there were significant elevations in SOD, nitric oxide (NO), glutathione-S-transferase (GST), and AChE activities compared with those in the C group. In the G+R group, LPO, NO, SOD, and GST activities were reduced compared with the R group. Conclusion: Our results show that gingipain dysregulated AChE activity and the oxidant-antioxidant balance in rotenone-exposed zebrafish, demonstrating its possible role in gut dysbiosis, neuroinflammation, and Parkinson’s disease.
dc.description.sponsorshipMarmara University
dc.identifier.citationİşlek, İ., Beler, M., Ünal, İ., Cansız, D., Emekli Alturfan, E. ve Köse, K. N. (2024). Virulence factor of porphyromonas gingivalis disrupts intestinal oxidative status and acetylcholinesterase activity in rotenone-exposed zebrafish. Experimed, 14(3), 139-145. http://dx.doi.org/10.26650/experimed.1456373
dc.identifier.doi10.26650/experimed.1456373
dc.identifier.endpage145
dc.identifier.issn2667-5846
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85213386836
dc.identifier.scopusqualityQ4
dc.identifier.startpage139
dc.identifier.urihttp://dx.doi.org/10.26650/experimed.1456373
dc.identifier.urihttps://hdl.handle.net/20.500.12511/13360
dc.identifier.volume14
dc.identifier.wosWOS:001386721400002
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorCansız, Derya
dc.institutionauthorid0000-0002-6274-801X
dc.language.isoen
dc.relation.ecinfo:eu-repo/grantAgreement/TDK-2023-10892
dc.relation.ispartofExperimed
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsAttribution-NonCommercial 4.0 International
dc.subjectAcetylcholinesterase
dc.subjectGingipain
dc.subjectOxidative Stress
dc.subjectPorphyromonas Gingivalis
dc.subjectRotenone
dc.subjectZebrafish
dc.titleVirulence factor of porphyromonas gingivalis disrupts intestinal oxidative status and acetylcholinesterase activity in rotenone-exposed zebrafish
dc.typeArticle

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