Porphyromonas gingivalis enhances the senescence-induced increase of 5-alpha reductase in gingival fibroblasts

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dc.authorid0000-0001-8990-5866
dc.contributor.authorÖzkan Karasu, Yerda
dc.contributor.authorOrbak, Recep
dc.contributor.authorKaşali, Kamber
dc.contributor.authorBerker, Ezel
dc.contributor.authorKantarcı, Alpdoğan
dc.date.accessioned2024-02-14T07:15:29Z
dc.date.available2024-02-14T07:15:29Z
dc.date.issued2023
dc.departmentİstanbul Medipol Üniversitesi, Diş Hekimliği Fakültesi, Periodontoloji Ana Bilim Dalı
dc.description.abstractObjectives: Aging is characterized by chronic inflammatory activity. Senescent cells increase with chronic inflammation and age-related pathologies, including periodontal disease. As a critical regulator of tissue inflammaging, we hypothesized that 5? reductase (5?R) is associated with periodontal disease and bacteria-induced senescence in gingival fibroblasts. Materials and methods: We recruited 36 patients with periodontitis, measured 5?R immunohistochemically before and after periodontal treatment, and compared the expression of 5?R in gingival biopsies from 12 healthy individuals. We then tested the impact of Porphyromonas gingivalis on gingival fibroblasts treated with or without D-galactose-induced cell senescence. We treated primary gingival fibroblasts with D-galactose-supplemented media (0 µM, 50 µM, 100 µM, 1 mM, 10 mM, 50 mM) to induce senescence. The expression of type 1 and type 2 5?R was analyzed with real-time PCR and immunocytochemistry. The levels of IL-6, IL-8, TNF-?, and MCP-1 in fibroblast cultures were evaluated by multiplex immunoassay. Results: In gingival biopsies from patients with periodontal disease, the expression of 5?R was significantly higher than in samples from individuals without periodontal disease (p < 0.001). Periodontal treatment significantly reduced the expression of 5?R in gingival tissues (p < 0.001) to levels comparable in healthy individuals. Gingival fibroblasts exposed to D-galactose-supplemented media had a dose-dependent and significant increase in 5?R expression (p < 0.001). P. gingivalis caused statistically higher type 1 and type 2 5?R expression in gingival fibroblast cells. This effect was exacerbated by the lower doses of D-galactose (p = 0.037). Cells infected with P. gingivalis produced significantly higher levels of IL-6, IL-8, TNF-?, and MCP-1 (p < 0.05) regardless of the D-galactose exposure. Conclusion: The results suggested that 5?R plays a role in periodontal disease and mediates the senescence-induced response to P. gingivalis in gingival fibroblasts. Clinical relevance: Periodontal diseases and aging can increase the production of 5-alpha reductase in the gingival tissue.
dc.identifier.citationÖzkan Karasu, Y., Orbak, R., Kaşali, K., Berker, E. ve Kantarcı, A. (2023). Porphyromonas gingivalis enhances the senescence-induced increase of 5-alpha reductase in gingival fibroblasts. Clinical Oral Investigations, 27(10), 5977-5989. https://dx.doi.org/10.1007/s00784-023-05211-y
dc.identifier.doi10.1007/s00784-023-05211-y
dc.identifier.endpage5989
dc.identifier.issn1432-6981
dc.identifier.issn1436-3771
dc.identifier.issue10
dc.identifier.pmid37608238
dc.identifier.scopus2-s2.0-85168609876
dc.identifier.scopusqualityQ1
dc.identifier.startpage5977
dc.identifier.urihttps://dx.doi.org/10.1007/s00784-023-05211-y
dc.identifier.urihttps://hdl.handle.net/20.500.12511/12273
dc.identifier.volume27
dc.identifier.wos001052774000002en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorBerker, Ezel
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofClinical Oral Investigationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/2214/A 1059B141600460
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subject5? Reductase
dc.subjectPeriodontal Disease
dc.subjectSenescence
dc.titlePorphyromonas gingivalis enhances the senescence-induced increase of 5-alpha reductase in gingival fibroblasts
dc.typeArticle

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