The optimal method to measure polyamines in serum by using HPLC fluorescence detector

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dc.authorid0000-0002-6183-5015
dc.authorid0000-0002-0757-9743
dc.authorid0000-0002-0675-1839
dc.contributor.authorÇakan, Orhan
dc.contributor.authorHacıhasanoğlu Çakmak, Neziha
dc.contributor.authorBayramoğlu, Feyza
dc.contributor.authorYiğitbaşı, Türkan
dc.date.accessioned2023-03-22T12:21:00Z
dc.date.available2023-03-22T12:21:00Z
dc.date.issued2023
dc.departmentİstanbul Medipol Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Laboratuvar Teknikleri Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.description.abstractMeasurement of polyamines (putrescine, spermidine and spermine) is important in the monitoring of many metabolic events, as well as in cancer prognosis. However, the lack of method standardization and validation for the measurement of poly-amines is a major obstacle. In this study, sample preparation, gradient program, chromatography column, sample concentration studies were attempted and an analysis method was developed for the detection of polyamines in serum by using HPLC fluorescence detector in order to reduce the difficulties experienced with the existing methodologies. This method was validated analytically in accordance with EMA and FDA’s guidelines. In this study, Waters Nova-Pak C18 A chromatography column with 3.9 mm, 150 mm and 4 µm specifications was used for the first time. Acetonitrile was used instead of methanol. Thanks to the newly developed gradient, peak purity and sufficient peak separation were ensured and accurate, sensitive, reliable and reproducible method was developed and validated.
dc.identifier.citationÇakan, O., Hacıhasanoğlu Çakmak, N., Bayramoğlu, F. ve Yiğitbaşı, T. (2023). The optimal method to measure polyamines in serum by using HPLC fluorescence detector. Acta Pharmaceutica Sciencia, 61(1), 91-106. https://dx.doi.org/10.23893/1307-2080.APS6107
dc.identifier.doi10.23893/1307-2080.APS6107
dc.identifier.endpage106
dc.identifier.issn1307-2080
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85149203618
dc.identifier.scopusqualityQ3
dc.identifier.startpage91
dc.identifier.urihttps://dx.doi.org/10.23893/1307-2080.APS6107
dc.identifier.urihttps://hdl.handle.net/20.500.12511/10661
dc.identifier.volume61
dc.indekslendigikaynakScopus
dc.institutionauthorHacıhasanoğlu Çakmak, Neziha
dc.institutionauthorBayramoğlu, Feyza
dc.institutionauthorYiğitbaşı, Türkan
dc.language.isoen
dc.publisherIstanbul Medipol University
dc.relation.ispartofActa Pharmaceutica Scienciaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDerivatization
dc.subjectHPLC
dc.subjectN-Acetyl-L Cysteine
dc.subjectO-Phthalaldehyde
dc.subjectPolyamines
dc.titleThe optimal method to measure polyamines in serum by using HPLC fluorescence detector
dc.typeArticle

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