Urtica dioica extract downregulates the gene expression of 5?-RII in hacat cells: Possible implications against androgenic skin diseases

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dc.authorid0000-0003-1469-2557
dc.contributor.authorPekmezci, Erkin
dc.contributor.authorTürkoğlu, M.
dc.date.accessioned2023-03-22T11:39:45Z
dc.date.available2023-03-22T11:39:45Z
dc.date.issued2023
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Deri ve Zührevi Hastalıklar Ana Bilim Dalı
dc.description.abstractUrtica dioica (Ud) is a perennial plant of temperate climate regions and has been reported therapeutic activity against benign prostate hyperplasia, mainly due to its 5-alpha-reductase (5?-R) inhibition feature, which has been singly shown only in prostatic tissues until now. Also considering its use in traditional medicine against some dermatological problems and hair loss, we performed an in-vitro study to reveal its 5?-R inhibition activity in skin cells whether this plant may have a therapeutic potential against androgenic skin diseases. After the preparation of Ud leaf extract and determination of non-cytotoxic concentration, cultured HaCaT cells were treated with the plant extract. RNA isolations were carried out from both non-treated and treated cell groups. cDNA synthesis was performed using gene specific primers of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as reference gene and 5?-R type II (5?-RII) as study material. Gene expressions were determined by real time reverse transcription quantitative polymerase chain reaction analysis. Results were represented as 'Target/GAPDH Fold Change'. Results of gene expression analysis showed that plant extract caused statistically significant downregulation of 5?-RII gene expression (p=0.0021) in treated cells, compared to untreated control cells, and ended up with 0.5873±0.0586 fold change. This study is the first one showing the suppression of 5?-RII gene expression on skin cells with unmixed or solitary Ud extract. With the currently reported anti-androgenic activity in HaCaT cells, it can be suggested that Ud has a solid scientific base and may have a promising future in cosmetic dermatology, and new product development against androgenic skin diseases.
dc.identifier.citationPekmezci, E. ve Türkoğlu, M. (2023). Urtica dioica extract downregulates the gene expression of 5?-RII in hacat cells: Possible implications against androgenic skin diseases. Georgian Medical News, 334, 6-9.
dc.identifier.endpage9
dc.identifier.issn1512-0112
dc.identifier.issue334
dc.identifier.pmid36864786
dc.identifier.scopus2-s2.0-85149329004
dc.identifier.scopusqualityQ4
dc.identifier.startpage6
dc.identifier.urihttps://hdl.handle.net/20.500.12511/10659
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorPekmezci, Erkin
dc.language.isoen
dc.publisherNLM (Medline)
dc.relation.ispartofGeorgian Medical Newsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject5?-RII
dc.subjectHacat Cells
dc.subjectGene Expression
dc.titleUrtica dioica extract downregulates the gene expression of 5?-RII in hacat cells: Possible implications against androgenic skin diseases
dc.typeArticle

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