Imaging of the axon initial segment

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dc.contributor.authorDi Re, Jessica
dc.contributor.authorKayasandık, Cihan Bilge
dc.contributor.authorBotello-Lins, Gonzalo
dc.contributor.authorLabate, Demetrio
dc.contributor.authorLaezza, Fernanda M.
dc.date.accessioned2019-12-25T19:36:40Z
dc.date.available2019-12-25T19:36:40Z
dc.date.issued2019
dc.departmentİstanbul Medipol Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Bilgisayar Mühendisliği Bölümü
dc.description.abstractThe axon initial segment (AIS) is the first 20- to 60-?m segment of the axon proximal to the soma of a neuron. This highly specialized subcellular domain is the initiation site of the action potential and contains a high concentration of voltage-gated ion channels held in place by a complex nexus of scaffolding and regulatory proteins that ensure proper electrical activity of the neuron. Studies have shown that dysfunction of many AIS channels and scaffolding proteins occurs in a variety of neuropsychiatric and neurodegenerative diseases, raising the need to develop accurate methods for visualization and quantification of the AIS and its protein content in models of normal and disease conditions. In this article, we describe methods for immunolabeling AIS proteins in cultured neurons and brain slices as well as methods for quantifying protein expression and pattern distribution using fluorescent labeling of these proteins.
dc.identifier.citationDi Re, J., Kayasandık, C. B., Botello-Lins, G., Labate, D. ve Laezza, F. M. (2019). Imaging of the axon initial segment. Current Protocols in Neuroscience, 89(1). https://doi.org/10.1002/cpns.78
dc.identifier.doi10.1002/cpns.78
dc.identifier.issn1934-8584
dc.identifier.issn1934-8576
dc.identifier.issue1
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1002/cpns.78
dc.identifier.urihttps://hdl.handle.net/20.500.12511/4700
dc.identifier.volume89
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBlackwell Publishing, Inc.
dc.relation.ispartofCurrent Protocols in Neuroscienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAxon Initial Segment
dc.subjectImaging
dc.subjectProtein Expression
dc.titleImaging of the axon initial segment
dc.typeArticle

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