Pregabalin treatment for neuropathic pain may damage intervertebral disc tissue

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dc.authorid0000-0003-2003-6337
dc.authorid0000-0002-8084-7855
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorYaşar Şirin, Duygu
dc.contributor.authorÖzbek, Hanefi
dc.contributor.authorKaplan, Necati
dc.contributor.authorKaya, Yasin Emre
dc.contributor.authorAkyuva, Yener
dc.contributor.authorGürbüz, Mehmet Sabri
dc.contributor.authorÖznam, Kadir
dc.contributor.authorAteş, Özkan
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:50:21Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T19:50:21Z
dc.date.issued2018
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Farmakoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Ortopedi ve Travmatoloji Ana Bilim Dalı
dc.descriptionWOS: 000442280500109
dc.descriptionPubMed ID: 30112057
dc.description.abstractThe aim of the present study was to determine whether pharmaceutical preparations with pregabalin (PGB) as an active ingredient, which are widely prescribed by clinicians, exert toxic effects on human primary nucleus pulposus (NP) and annulus fibrosis (AF). Primary human cell cultures were obtained from intact (n=6) and degenerated (n=6) tissues resected from the two groups of patients. Different doses of PGB were applied to these cultures and cells were subjected to molecular analyses at 0, 24 and 48 h. Cell vitality, toxicity and proliferation were assessed using a spectrophotometer. The expression of chondroadherin (CHAD), a (member of the NP-specific protein family), hypoxia-inducible factor-1 alpha (HIF-1 alpha) and type II collagen (COL2A1) was measured using reverse transcription-quantitative polymerase chain reaction. The results revealed that cell intensity increased in a time-dependent manner and cell vitality continued in the cultures without pharmaceuticals. Cell proliferation was suppressed in the PGB-treated cultures independent from the dose and duration of application. PGB was demonstrated to suppress the expression of CHAD and HIF-1 alpha. In contrast, COL2A1 gene expression was not revealed in any experimental group. The present study utilized an in vitro model and the PGB active ingredient used herein may not be representative of clinical applications; however, the results demonstrated that PGB has a toxic effect on NP/AF cell cultures containing primary human intervertebral disc tissue. In summary, the use of pharmacological agents containing PGB may suppress the proliferation and differentiation of NP/AF cells and/or tissues, which should be considered when deciding on an appropriate treatment regime.
dc.identifier.citationKaraarslan, N., Yılmaz, İ., Yaşar Şirin, D., Özbek, H., Kaplan, N., Kaya, Y. ... Ateş, Ö. (2018). Pregabalin treatment for neuropathic pain may damage intervertebral disc tissue. Experimental and Therapeutic Medicine, 16(2), 1259-1265. https://dx.doi.org/10.3892/etm.2018.6289
dc.identifier.doi10.3892/etm.2018.6289
dc.identifier.endpage1265
dc.identifier.issn1792-0981
dc.identifier.issn1792-1015
dc.identifier.issue2
dc.identifier.scopusqualityQ2
dc.identifier.startpage1259
dc.identifier.urihttps://dx.doi.org/10.3892/etm.2018.6289
dc.identifier.urihttps://hdl.handle.net/20.500.12511/1960
dc.identifier.volume16
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpandidos Publications Ltd
dc.relation.ispartofExperimental and Therapeutic Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/legalcode*
dc.subjectAnnulus Fibrosis
dc.subjectChondroadherin
dc.subjectType II Collagen
dc.subjectHypoxia-Inducible Factor-1 Alpha
dc.subjectIntervertebral Disc
dc.subjectNucleus Pulposus
dc.subjectPregabalin
dc.subjectPrimary Cell Culture
dc.subjectCytotoxicity
dc.titlePregabalin treatment for neuropathic pain may damage intervertebral disc tissue
dc.typeArticle

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