Identification and molecular characterization of Otobius megnini (Ixodida: Argasidae) seen in humans in Mus province, Turkey

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dc.authorid0000-0001-7984-9266
dc.contributor.authorKarakuş, Ayşe
dc.contributor.authorYılmaz, Ali Bilgin
dc.contributor.authorKaraca, Servet
dc.contributor.authorDik, Bilal
dc.contributor.authorDenizhan, Vural
dc.date.accessioned2022-03-03T07:54:17Z
dc.date.available2022-03-03T07:54:17Z
dc.date.issued2022
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Plastik, Rekonstrüktif ve Estetik Cerrahi Ana Bilim Dalı
dc.description.abstractOtobius megnini (Ixodida: Argasidae) is a cosmopolitan soft tick that parasitizes humans as well as domestic and wild animals. The larval and nymph stages of this tick usually feed by parasitizing in the ear canal. The material of this study consists of ticks collected during ear cleaning in approximately 496 people coming with the complaint of ear pain in state hospitals in Bulanik and Malazgirt districts of Mud province, eastern Turkey. As a result of microscopic examination performed on ticks collected from humans, O. megnini tick was determined and molecular identification was made for definitive diagnosis. The 16S rRNA gene fragment of the tick was amplified by PCR. Obtained PCR products were 360 bp. The PCR products were analyzed by sequence analysis and compared with the reference sequences in the BLAST and Genbank. A phylogenetic tree was created with MEGA 7 software using Maximum Likelihood model. As a result, the previously identified Otobius megnini in Turkey, was confirmed using molecular methods for the first time.
dc.identifier.citationKarakuş, A., Yılmaz, A. B., Karaca, S., Dik, B. ve Denizhan, V. (2022). Identification and molecular characterization of Otobius megnini (Ixodida: Argasidae) seen in humans in Mus province, Turkey. Persian Journal of Acarology, 11(1), 145-152. https://doi.org/10.22073/pja.v11i1.71113
dc.identifier.doi10.22073/pja.v11i1.71113
dc.identifier.endpage152
dc.identifier.issn2251-8169
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85127711636
dc.identifier.scopusqualityQ3
dc.identifier.startpage145
dc.identifier.urihttps://doi.org/10.22073/pja.v11i1.71113
dc.identifier.urihttps://hdl.handle.net/20.500.12511/9081
dc.identifier.volume11
dc.identifier.wos000753611700012en_US
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorKaraca, Servet
dc.language.isoen
dc.publisherAcarological Society of Iran
dc.relation.ispartofPersian Journal of Acarologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subject16S rRNA
dc.subjectAcari
dc.subjectEar Tick
dc.subjectPCR
dc.subjectSoft Tick
dc.titleIdentification and molecular characterization of Otobius megnini (Ixodida: Argasidae) seen in humans in Mus province, Turkey
dc.typeArticle

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