Effects of methylprednisolone in the treatment of spinal cord injuries by evaluation of microRNA-21: An experimental study

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dc.authorid0000-0001-5401-1547
dc.contributor.authorAbdallah, Anas
dc.contributor.authorTekin, Abdurrahim
dc.contributor.authorÖztanır, Mustafa Namık
dc.contributor.authorSüsgün, Seda
dc.contributor.authorYabacı, Ayşegül
dc.contributor.authorÇınar, İrfan
dc.contributor.authorCan, Engin
dc.contributor.authorTokar, Sadık
dc.contributor.authorAkbaş, Fahri
dc.contributor.authorSeyithanoğlu, Mehmet Hakan
dc.date.accessioned2023-09-21T11:19:38Z
dc.date.available2023-09-21T11:19:38Z
dc.date.issued2023
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Beyin ve Sinir Cerrahisi Ana Bilim Dalı
dc.description.abstractBackground and Study Aims Spinal cord injury (SCI) is one of the most complicated pathologies that affect active young males. miR-21 primarily regulates several cellular processes. We aimed to elucidate the regulatory role of miR-21 and test methylprednisolone as a disease-modifying agent on experimental SCI tissues. Methods A total of 36 8- to 10-week-old adult female Sprague-Dawley rats weighing 250 to 300 g were used. Animals were randomly divided into six groups. Except for groups 1 and 4, the spinal trauma model was applied to all animal groups using the clipping method. In groups 3 and 6, methylprednisolone was given. For real-time polymerase chain reaction (PCR) investigations, rats in groups 1, 2, and 3 were reoperated on after the first postoperative day, whereas those in groups 4, 5, and 6 were reoperated on after postoperative day 7 and spinal cord samples from the laminectomy area were removed for gene expression analysis. Relative gene expression of miR-21, Gfap , Vim , Stat3 , Faslg , Pten , Bax , Bcl2 , Cox2 , and Il6 were determined with quantitative reverse transcription (qRT) PCR. Results In group 3, the miR-21 expression significantly increased compared with groups 1 and 2. When compared with group 3, a decrease in miR-21 expression was observed in group 6 ( p < 0.05). When compared with group 4, group 6 had lower levels of Gfap , Pten , Stat3 , and Bax ( p < 0.05). Conclusions miR-21 supports the beneficial aspects of the body's healing mechanisms following SCI. In the acute phase, the use of methylprednisolone increases miR-21 expression in the early period of trauma. Methylprednisolone increases some astrogliosis and inflammation biomarkers' levels; however, it did not affect the apoptotic biomarkers.
dc.identifier.citationAbdallah, A., Tekin, A., Öztanır, M. N., Süsgün, S., Yabacı, A., Çınar, İ. ... Seyithanoğlu, M. H. (2023). Effects of methylprednisolone in the treatment of spinal cord injuries by evaluation of microRNA-21: An experimental study. Journal of Neurological Surgery, Part A: Central European Neurosurgery, 84(3), 240-246. https://doi.org/10.1055/s-0042-1743552
dc.identifier.doi10.1055/s-0042-1743552
dc.identifier.endpage246
dc.identifier.issn2193-6315
dc.identifier.issn2193-6323
dc.identifier.issue3
dc.identifier.pmid35439827
dc.identifier.scopus2-s2.0-85128930852
dc.identifier.scopusqualityQ3
dc.identifier.startpage240
dc.identifier.urihttps://doi.org/10.1055/s-0042-1743552
dc.identifier.urihttps://hdl.handle.net/20.500.12511/11481
dc.identifier.volume84
dc.identifier.wos000783930100001en_US
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorTekin, Abdurrahim
dc.language.isoen
dc.publisherGeorg Thieme Verlag
dc.relation.ispartofJournal of Neurological Surgery, Part A: Central European Neurosurgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectSpinal Cord Injury
dc.subjectmiR-21
dc.subjectMethylprednisolone
dc.subjectReal-Time PCR
dc.titleEffects of methylprednisolone in the treatment of spinal cord injuries by evaluation of microRNA-21: An experimental study
dc.typeArticle

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