Instant determination of the artemisinin from various Artemisia annua L. extracts by LC-ESI-MS/MS and their in-silico modelling and in vitro antiviral activity studies against SARS-CoV-2

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dc.authorid0000-0001-6342-4298
dc.authorid0000-0002-1423-0435
dc.contributor.authorDoğan, Kübra
dc.contributor.authorErol, Ebru
dc.contributor.authorOrhan, Müge Didem
dc.contributor.authorDeğirmenci, Zehra
dc.contributor.authorKan, Tuğçe
dc.contributor.authorGüngör, Ayşen
dc.contributor.authorYaşa, Belkıs
dc.contributor.authorAvşar, Timuçin
dc.contributor.authorÇetin, Yüksel
dc.contributor.authorDurdağı, Serdar
dc.contributor.authorGüzel, Mustafa
dc.date.accessioned2023-01-02T10:41:05Z
dc.date.available2023-01-02T10:41:05Z
dc.date.issued2022
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü
dc.departmentİstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Farmakoloji Ana Bilim Dalı
dc.description.abstractIntroduction Numerous efforts in natural product drug development are reported for the treatment of Coronavirus. Based on the literature, among these natural plants Artemisia annua L. shows some promise for the treatment of SARS-CoV-2. Objective The main objective of our study was to determine artemisinin content by liquid chromatography electrospray ionisation tandem mass spectrometry (LC-ESI-MS/MS), to investigate the in vitro biological activity of artemisinin from the A. annua plants grown in Turkey with various extracted methods, to elaborate in silico activity against SARS-CoV-2 using molecular modelling. Methodology Twenty-one different extractions were applied. Direct and sequential extractions studies were compared with ultrasonic assisted maceration, Soxhlet, and ultra-rapid determined artemisinin active molecules by LC-ESI-MS/MS methods. The inhibition of spike protein and main protease (3CL) enzyme activity of SARS-CoV-2 virus was assessed by time resolved fluorescence energy transfer (TR-FRET) assay. Results Artemisinin content in the range 0.062-0.066%. Artemisinin showed significant inhibition of 3CL protease activity but not Spike/ACE-2 binding. The 50% effective concentration (EC50) of artemisinin against SARS-CoV-2 Spike pseudovirus was found greater than 50 mu M (EC45) in HEK293T cell line whereas the cell viability was 94% of the control (P < 0.01). The immunosuppressive effects of artemisinin on TNF-alpha production on both pseudovirus and lipopolysaccharide (LPS)-induced THP-1 cells were found significant in a dose dependent manner. Conclusion Further studies of these extracts for COVID-19 treatment will shed light to seek alternative treatment options. Moreover, these natural extracts can be used as an additional treatment option with medicines, as well as prophylactic use can be very beneficial for patients.
dc.identifier.citationDoğan, K., Erol, E., Orhan, M. D., Değirmenci, Z., Kan, T., Güngör, A. ... Güzel, M. (2022). Instant determination of the artemisinin from various Artemisia annua L. extracts by LC-ESI-MS/MS and their in-silico modelling and in vitro antiviral activity studies against SARS-CoV-2. Phytochemical Analysis, 33(2), 303-319. https://doi.org/10.1002/pca.3088
dc.identifier.doi10.1002/pca.3088
dc.identifier.endpage319
dc.identifier.issn0958-0344
dc.identifier.issn1099-1565
dc.identifier.issue2
dc.identifier.pmid34585460
dc.identifier.scopus2-s2.0-85115836341
dc.identifier.scopusqualityQ1
dc.identifier.startpage303
dc.identifier.urihttps://doi.org/10.1002/pca.3088
dc.identifier.urihttps://hdl.handle.net/20.500.12511/10223
dc.identifier.volume33
dc.identifier.wos000700840000001en_US
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorErol, Ebru
dc.institutionauthorGüzel, Mustafa
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofPhytochemical Analysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/18AG003
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAntiviral Activity
dc.subjectArtemisia Annua SARS-Cov-2
dc.subjectLC-ESI-MS/MS
dc.subjectNatural Products For COVID-19
dc.titleInstant determination of the artemisinin from various Artemisia annua L. extracts by LC-ESI-MS/MS and their in-silico modelling and in vitro antiviral activity studies against SARS-CoV-2
dc.typeArticle

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