Exposure to tumescent solution significantly increases phosphorylation of perilipin in adipocytes

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dc.authorid0000-0002-7059-1884
dc.authorid0000-0001-5106-0159
dc.authorid0000-0003-2066-7454
dc.contributor.authorKeskin, İlknur
dc.contributor.authorSütçü, Mustafa
dc.contributor.authorEren, Hilal
dc.contributor.authorKeskin, Mustafa
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T19:37:42Z
dc.date.available10.07.201910:49:14
dc.date.available2019-07-10T19:37:42Z
dc.date.issued2017
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalı
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Plastik, Rekonstrüktif ve Estetik Cerrahi Ana Bilim Dalı
dc.description.abstractBACKGROUND: Lidocaine and epinephrine could potentially decrease adipocyte viability, but these effects have not been substantiated. The phosphorylation status of perilipin in adipocytes may be predictive of cell viability. Perilipin coats lipid droplets and restricts access of lipases; phospho-perilipin lacks this protective function.OBJECTIVES: The authors investigated the effects of tumescent solution containing lidocaine and epinephrine on the phosphorylation status of perilipin in adipocytes.METHODS: In this in vitro study, lipoaspirates were collected before and after tumescence from 15 women who underwent abdominoplasty. Fat samples were fixed, sectioned, and stained for histologic and immunohistochemical analyses. Relative phosphorylation of perilipin was inferred from pixel intensities of immunostained adipocytes observed with confocal microscopy.RESULTS: For adipocytes collected before tumescent infiltration, 10.08% of total perilipin was phosphorylated. In contrast, 30.62% of total perilipin was phosphorylated for adipocytes collected from tumescent tissue (P < .01).CONCLUSIONS: The tumescent technique increases the relative phosphorylation of perilipin in adipocytes, making these cells more vulnerable to lipolysis. Tumescent solution applied for analgesia or hemostasis of the donor site should contain the lowest possible concentrations of lidocaine and epinephrine. LEVEL OF EVIDENCE 5.
dc.identifier.citationKeskin, İ., Sütçü, M., Eren, H. ve Keskin, M. (2017). Exposure to tumescent solution significantly increases phosphorylation of perilipin in adipocytes. Aesthetic Surgery Journal, 37(2), 239-245. https://dx.doi.org/10.1093/asj/sjw156
dc.identifier.doi10.1093/asj/sjw156
dc.identifier.endpage245
dc.identifier.issn1527-330X
dc.identifier.issn1090-820X
dc.identifier.issue2
dc.identifier.scopusqualityQ1
dc.identifier.startpage239
dc.identifier.urihttps://hdl.handle.net/20.500.12511/1455
dc.identifier.urihttps://dx.doi.org/10.1093/asj/sjw156
dc.identifier.volume37
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.relation.ispartofAesthetic Surgery Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBackground
dc.subjectObjectives
dc.subjectMethods
dc.subjectResults
dc.subjectConclusions
dc.titleExposure to tumescent solution significantly increases phosphorylation of perilipin in adipocytes
dc.typeArticle

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