Conditioned medium derived from neural progenitor cells induces long-term post-ischemic neuroprotection, sustained neurological recovery, neurogenesis, and angiogenesis

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dc.contributor.authorDoeppner, Thorsten R.
dc.contributor.authorTraut, Viktorija
dc.contributor.authorHeidenreich, Alexander
dc.contributor.authorKaltwasser, Britta
dc.contributor.authorBosche, Bert
dc.contributor.authorBahr, Mathias
dc.contributor.authorHermann, Dirk M.
dc.date.accessioned10.07.201910:49:13
dc.date.accessioned2019-07-10T20:01:50Z
dc.date.available10.07.201910:49:13
dc.date.available2019-07-10T20:01:50Z
dc.date.issued2017
dc.departmentİstanbul Medipol Üniversitesi, Rektörlük, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi (REMER)
dc.descriptionWOS: 000394964100059
dc.descriptionPubMed ID: 26860410
dc.description.abstractAdult neural progenitor cells (NPCs) induce post-ischemic long-term neuroprotection and brain remodeling by releasing of survival- and plasticity-promoting mediators. To evaluate whether secreted factors may mimic neuroprotective and restorative effects of NPCs, we exposed male C57BL6 mice to focal cerebral ischemia and intravenously applied conditioned medium (CM) derived from subventricular zone NPCs. CM dose-dependently reduced infarct volume and brain leukocyte infiltration after 48 h when delivered up to 12 h after focal cerebral ischemia. Neuroprotection persisted in the post-acute stroke phase yielding enhanced neurological recovery that lasted throughout the 28-day observation period. Increased Bcl-2, phosphorylated Akt and phosphorylated STAT-3 abundance, and reduced caspase-3 activity and Bax abundance were noted in ischemic brains of CM-treated mice at 48 h post-stroke, indicative of enhanced cell survival signaling. Long-term neuroprotection was associated with increased brain glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF) concentrations at 28 days resulting in increased neurogenesis and angiogenesis. The observation that NPC-derived CM induces sustained neuroprotection and neurological recovery suggests that cell transplantation may be dispensable when secreted factors are instead administered.
dc.description.sponsorshipTUBITAK [2221]; German Research Council [HE3173/2-2, HE3173/3-1]en_US
dc.description.sponsorshipThe present study was supported by TUBITAK (#2221; to TRD) and the German Research Council (HE3173/2-2 and HE3173/3-1; to DMH).en_US
dc.identifier.citationDoeppner, T. R., Traut, V., Heidenreich, A., Kaltwasser, B., Bosche, B., Bahr, M. ... Hermann, D. M. (2017). Conditioned medium derived from neural progenitor cells induces long-term post-ischemic neuroprotection, sustained neurological recovery, neurogenesis, and angiogenesis. Molecular Neurobiology, 54(2), 1531-1540. https://dx.doi.org/10.1007/s12035-016-9748-y
dc.identifier.doi10.1007/s12035-016-9748-y
dc.identifier.endpage1540
dc.identifier.issn0893-7648
dc.identifier.issn1559-1182
dc.identifier.issue2
dc.identifier.scopusqualityQ1
dc.identifier.startpage1531
dc.identifier.urihttps://dx.doi.org/10.1007/s12035-016-9748-y
dc.identifier.urihttps://hdl.handle.net/20.500.12511/3462
dc.identifier.volume54
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ecinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/2221
dc.relation.ispartofMolecular Neurobiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectCerebral Ischemia
dc.subjectConditioned Medium
dc.subjectNeural Progenitor Cells
dc.subjectNeuroregeneration
dc.subjectStroke
dc.titleConditioned medium derived from neural progenitor cells induces long-term post-ischemic neuroprotection, sustained neurological recovery, neurogenesis, and angiogenesis
dc.typeArticle

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