Morphine attenuates neurotoxic effects of MPTP in zebrafish embryos by regulating oxidant/antioxidant balance and acetylcholinesterase activity

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dc.authorid0000-0003-0804-1475
dc.contributor.authorCansız, Derya
dc.contributor.authorÜstündağ, Ünsal Veli
dc.contributor.authorÜnal, İsmail
dc.contributor.authorAlturfan, Ahmet Ata
dc.contributor.authorEmekli Alturfan, Ebru
dc.date.accessioned2023-01-04T10:54:20Z
dc.date.available2023-01-04T10:54:20Z
dc.date.issued2022
dc.departmentİstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyokimya Ana Bilim Dalı
dc.description.abstractParkinson's disease (PD) is one of the most common neurodegenerative diseases due to the loss of dopaminergic neurons in the midbrain in the substantia nigra. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is a neurotoxic agent causing disruptions in mitochondria of dopaminergic neurons leading to impaired oxidant-antioxidant balance. Both zebrafish and zebrafish embryos are sensitive to MPTP. In zebrafish embryos, MPTP decreases the dopaminergic cells in the diencephalon by damaging dopaminergic neurons. Morphine is an opioid pain killer and a strong analgesic that is used to treat chronic pain. Until today morphine has been shown to regulate the survival or death of neurons and both protective and destructive effects of morphine have been reported in the central nervous system. This study aimed to evaluate the effects of morphine in MPTP-exposed zebrafish embryos. Developmental parameters were monitored and documented daily during embryonic development. Locomotor activity of zebrafish embryos at 96 h postfertilization (hpf) was determined. Acetylcholinesterase (AChE) activity and oxidant-antioxidant parameters were analyzed by biochemical methods. RT-PCR was used to evaluate bdnf, dj1, lrrk and pink1 expressions. Morphine treatment improved mortality and hatching rates, locomotor activity, AChE, and antioxidant enzyme activities as well as the expressions of bdnf, dj1, lrrk and pink1 in a dose-dependent manner that were altered by MPTP. Increased lipid peroxidation supports the role of morphine to induce autophagy to prevent PD-related pathologies. Our study provided important data on the possible molecular mechanism of the therapeutic effects of morphine in PD.
dc.identifier.citationCansız, D., Üstündağ, Ü. V., Ünal, İ., Alturfan, A. A. ve Emekli Alturfan, E. (2022). Morphine attenuates neurotoxic effects of MPTP in zebrafish embryos by regulating oxidant/antioxidant balance and acetylcholinesterase activity. Drug and Chemical Toxicology, 45(6), 2439-2447. https://dx.doi.org/10.1080/01480545.2021.1957558
dc.identifier.doi10.1080/01480545.2021.1957558
dc.identifier.endpage2447
dc.identifier.issn0148-0545
dc.identifier.issn1525-6014
dc.identifier.issue6
dc.identifier.pmid34340603
dc.identifier.scopus2-s2.0-85111819236
dc.identifier.scopusqualityQ3
dc.identifier.startpage2439
dc.identifier.urihttps://dx.doi.org/10.1080/01480545.2021.1957558
dc.identifier.urihttps://hdl.handle.net/20.500.12511/10238
dc.identifier.volume45
dc.identifier.wos000680255100001en_US
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÜstündağ, Ünsal Veli
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofDrug and Chemical Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectMorphine
dc.subjectMPTP
dc.subjectParkinson’s Disease
dc.subjectLocomotor Activity
dc.subjectBDNF
dc.subjectZebrafish Embryo
dc.titleMorphine attenuates neurotoxic effects of MPTP in zebrafish embryos by regulating oxidant/antioxidant balance and acetylcholinesterase activity
dc.typeArticle

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