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Öğe Replating protocol for human induced pluripotent stem cell–derived cardiomyocytes(Humana Press Inc., 2022) Koç, Arzuhan; Çağavi, EsraHuman induced pluripotent stem cell–derived cardiomyocytes (hiPSC-CM) create an unlimited cell source for basic and translational cardiac research. Obtaining hiPSC-CM culture as a single-cell, monolayer or three-dimensional clusters for downstream applications can be challenging. Thus, it is critical to develop replating strategies for hiPSC-CMs by evaluating different enzymatic or nonenzymatic reagents for dissociation and seeding on different coating materials. To reseed hiPSC-CMs with high viability and at structures desirable for the downstream applications, here we defined optimized protocols to dissociate hiPSC-CMs by using collagenase A&B, Collagenase II, TrypLE, and EDTA and reseeding on various matrix materials including fibronectin, laminin, imatrix, Matrigel, and Geltrex. By the replating methods described here, a single cell or cluster-containing hiPSC-CM cultures can be generated effectively.Öğe Revealing nervous and cardiac system interactions by iPSC-Based platforms(Elsevier, 2022) Mutlu Burnaz, Özlem; Çağavi, EsraCardiac function is regulated by the autonomic nervous system. In exchange, the sensorial information from the heart is relayed to the brain via sensory neurons as a crucial modulatory feedback mechanism. Cardiovascular and neurological diseases constitute the majority of deaths globally. The high morbidity associated with cardiac and neurological disorders is mostly due to the limited number of targeted therapeutics. Moreover, many new drug candidates are withdrawn from clinical use due to cardiotoxicity or neurotoxicity. Previously, experimental animal models, biopsy materials, or immortalized cell lines were the basis of disease studies and drug screens. However, the differences between these models and human physiology particularly in neural and cardiac functions resulted in limited clinical success. To overcome the complications related to the organism mismatch and cell source, human induced pluripotent stem cells (hiPSCs) provide ways to investigate molecular mechanisms in embryonic, adult, and diseased states. hiPSC-derived cardiac cells and various neuron subtypes could replicate complex interactions in the physiologically relevant organoids or multiorgan microdevices. Using these novel technical developments, recent models of neuronal regulation of heart tissue started to provide unique insights in systemic interactions and molecular basis to develop more precise therapeutic approaches. In this chapter, a brief historical perspective and recent advances in iPSC-based models of cardiac and nervous system interactions are reviewed.
