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  • Küçük Resim
    Öğe
    Activation of matrix-bound endogenous proteases by self-etch adhesives
    (NLM (Medline), 2020) Oğuz Ahmet, Bebek Serra; Şeşeoğulları Dirihan, Roda; Tezvergil Mutluay, Arzu
    The study evaluated changes in total enzymatic activity and degradation of demineralized dentin following the application of universal or self-etch adhesives. The universal adhesives -Scotchbond Universal (SU) and All-Bond Universal (ABU) and self-etch adhesives -Adper Easy Bond (EB) and G-aenial Bond (GB) were used for 2 min pretreatment of the dentin beams. Phosphoric acid (PA) treatment as well as no treatment served as controls. Total enzymatic activity was analyzed before and after treatment, collagen degradation was assessed using mass loss, C-terminal telopeptide (CTX) and C-terminal-telopeptide of type I collagen (ICTP) release (24 h, 3-day, 3-week). Over three weeks of incubation, ICTP release of ABU treated beams was significantly higher than other groups (p<0.05), except for SU treated beams (p>0.05) and CTX release of GB treated beams was the highest among the groups with statistically significant difference (p<0.05). The results confirm that the universal adhesives tested have also potential to increase the enzymatic activity in dentin.
  • Küçük Resim Yok
    Öğe
    Effect of acid or laser treatment on degradation of dentin matrix
    (Springer, 2022) Üşümez, Aslıhan; Sarı, Tuğrul; Şeşeoğulları Dirihan, Roda; Güven, Mehmet Esad; Oğuz Ahmet, Serra; Gutknecht, Norbert; Tezvergil Mutluay, Arzu
    Purpose: The objective of this study was to determine the effect of dental laser (DL) irradiation on the metalloproteinases (MMP)-mediated dentin collagen degradation. Methods: Human dentin disks were prepared (0.3 × 5x6mm, n = 60) and subjected to different treatments (n = 10/group): (1) control group (no treatment); (2) application of 37% phosphoric acid (PA) for 15 s; (3) irradiation with Er:YAG laser (120 mJ, 10 Hz); (4) irradiation with Er:YAG laser (120 mJ, 20 Hz); (5) irradiation with Er,Cr:YSGG laser (130mj, 30 Hz); and (6) irradiation with Er,Cr:YSGG laser (80mj, 50 Hz). After treatment, the total MMP activity and dry mass of each beam were measured, and after rehydration, they were incubated in 1 mL calcium- and zinc-containing incubation media for (a) one week and (b) three weeks. After each time point, dry mass changes were reassessed, and the aliquots of the incubation media were analyzed for pyridinoline-cross-link-containing degradation fragment of the C-terminal telopeptide of type I collagen (ICTP) using ELISA kits. All data were compared using ANOVA and Tukey’s test. Results: ANOVA showed significant differences among tested groups (p < 0.001). Laser-treated beams showed 55–75% less activity compared to 37% phosphoric acid-treated beams. The highest ICTP values were seen when PA was applied to the dentin surface in both time periods (p < 0.05). DL irradiation, be it with Er:YAG or Er,Cr:YSGG laser, did not increase the ICTP values in both time periods when compared with the control group (p > 0.05). Conclusion: The MMP-mediated dentin collagen degradation was the highest when PA was applied to the dentin surface and the lowest in Er:YAG or Er,Cr:YSGG laser-treated dentin groups in both time periods.
  • Küçük Resim
    Öğe
    Inhibition of cathepsin-K and matrix metalloproteinase by photodynamic therapy
    (Elsevier Inc., 2021) Kara, Özlem; Şeşeoğulları Dirihan, Roda; Sayın Özel, Gülsüm; Tezvergil Mutluay, Arzu; Üşümez, Aslıhan
    Objectives: The objective of this study was to determine the effects of antimicrobial photodynamic therapy (aPDT) with indocyanine green (ICG) and toluidine blue (TB) on protease activity (matrix-bound cathepsin K and matrix metalloproteinase (MMP) and dentin bond strength. Methods: Caries-free human third molars were assigned to five groups: 1—control group, 2—application of ICG with activation using an 810 nm diode (aPDT), 3—application of ICG, 4—application of TB with activation using a 660 nm diode (aPDT), and 5—application of TB. For the enzymatic investigation, dentin beams were incubated for either 3 days or 3 weeks. Aliquots of the incubation media were analyzed by ELISA for CTX (C-terminal cross-linked telopeptide of type I Collagen) and ICTP (cross-linked carboxy-terminal telopeptide of type I collagen). For microtensile bond strength testing (?TBS), composite resins were layered onto the tooth surface; the samples were then subjected to ?TBS. Kruskall–Wallis and Mann–Whitney U tests were applied for statistical analysis of CTX and ICTP, one way-ANOVA and Tukey's test were applied for statistical analysis of ?TBS. Results: Pretreating the dentin matrices with aPDT decreased the endogenous protease activity. ICG with laser activation resulted in the highest ?TBS. Therefore, aPDT should be considered as a treatment method because it can reduce MMP-mediated dentin degradation and increase the ?TBS. Significance: Inhibiting endogenous protease activity improves the stability of the dentin–adhesive bond and the durability of the bond strength.