Yazar "Sipahi, Hande" seçeneğine göre listele
Listeleniyor 1 - 14 / 14
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe A novel approach in the treatment of osteoarthritis: In vitro and in vivo evaluation of Allium sativum microemulsion(Wiley, 2022) Okur, Mehmet Evren; Kolbaşı, Bircan; Şahin, Mustafa; Karadağ, Ayşe Esra; Reis, Rengin; Çağlar, Emre Şefik; Kaplan, Arife Ahsen; Sipahi, Hande; Keskin, İlknur; Demiralp, Bahtiyar; Üstündağ Okur, NeslihanOsteoarthritis (OA) is the most prevalent kind of joint disease, affecting millions of individuals worldwide. Allium sativum L. was used as a therapeutic agent in the microemulsion system against osteoarthritis due to allicin's high antioxidant and anti-inflammatory effects. HPLC was used to evaluate the chemical components of the Allium sativum methanol extract. Water-in-oil (w/o) type of microemulsions loaded with Allium sativum extract was produced. To create the microemulsions, a titration approach was employed. The microemulsions were prepared using isopropyl myristate (IPM) as oil, Polysorbate 60, Sorbitan monolaurate 80 as surfactants, and Polyethylene glycol 400, ethanol as cosurfactants. The pH, droplet size, viscosity, polydispersity index values were measured to characterize of microemulsions. In addition, L929 healthy mouse fibroblasts and CHON-001 healthy human cartilage fibroblast cells were tested for cytocompatibility in vitro. The therapeutic potential of Allium sativum microemulsion was investigated in rats that had osteoarthritis surgically simulated. HPLC analysis revealed a significant concentration of allicin in the extract. The titration approach was used to create an A. sativum microemulsion (AS) with a narrow droplet size and PDI range. In L929 and CHON-001 cells, cytocompatibility experiments revealed that the maximum dosages of AS microemulsion 5% (v/v) exerted 93.41 +/- 3.11% and 75.59 +/- 2.69% cell viabilities, respectively. Different concentrations of AS (1.25%, 2.5%, and 5% v/v) demonstrated cytoprotective efficacy against H2O2-induced oxidative damage. When compared to the control group, in vivo investigations revealed fewer irregularities on the articular surface and typically arranged chondrocytes in the positive control and treated groups.Öğe Anti-inflammatory, analgesic and in vivo-in vitro wound healing potential of the Phlomis rigida Labill. extract(Elsevier Ireland Ltd, 2021) Okur, Mehmet Evren; Karadağ, Ayşe Esra; Özhan, Yağmur; Sipahi, Hande; Ayla, Şule; Daylan, Benay; Kültür, Şükran; Demirci, Betül; Demirci, FatihEthnopharmacological relevance: The preparations of Phlomis aerial parts are used traditionally in Anatolia for wound healing and in inflammatory disorders. Methods: For the identification of the active fraction, the air dried aerial parts of Phlomis rigida Labill. were extracted by methanol and fractionated successively by n-hexane, dichloromethane and ethyl acetate, respectively. The phenolic constituents were characterized by the Folin-Ciocaltheu method; the antioxidant activity was performed by ABTS and DPPH radical scavenging assays. In vitro anti-inflammatory activity was evaluated by LOX enzyme inhibition, spectrophotometrically as well as cell cultures. The wound healing properties of P. rigida extract gels were studied via in vitro cell culture methods and in vivo by excisional wound model using Balb-c mice. The P. rigida extract was analyzed and characterized by GC-FID, GC-MS, and LC-MS. Results: The P. rigida methanol extract showed moderate LOX inhibitory at IC50 = 19.5 ± 2.8 ?g/mL whereas the antioxidant activity was by DPPH• IC50 = 0.89 mg/mL, and by ABTS• IC50 = 0.99 mg/mL, respectively. In addition, a remarkable P. rigida extracts anti-inflammatory activity was observed in the cell culture assay, which was then confirmed by the in vitro wound healing activity applied at 0.125–0.5 mg/mL concentrations, resulting in a dose-dependent increase in wound closure at the final stage. The P. rigida gel formulation was prepared to evaluate the extract in vivo, whereas the experimental results of the new gel formulation supported the findings of the in vitro wound healing activity. Conclusion: The findings of this in vitro and in vivo study suggest that the wound healing and anti-inflammatory properties provide a scientific evidence of the ethnopharmacological application of Phlomis species.Öğe Biocompatibility of biomimetic multilayered alginate-chitosan/beta-TCP scaffold for osteochondral tissue(Elsevier Science, 2015) Algul, Derya; Sipahi, Hande; Aydın, Ahmet; Kelleci, Feyza; Özdatlı, Şükran; Yener, Fatma GülgünBiomimetic three-layered monolithic scaffold (TLS) intended for treatment of osteochondral defects was fabricated by using freeze drying method. The multilayered material was prepared with chitosan (C) and alginate (A) polyelectrolyte complex (CA/PEC) as a cartilaginous layer, a combination of CA/PEC (60 wt%) and beta-tricalcium phosphate (beta-TCP) (40 wt%) as an intermediate layer and a combination of CA/PEC (30 wt%) and beta-TCP (70 wt%) as a subchondral layer in order to mimic the inherent gradient structure of healthy osteochondral tissue. Characterization of the scaffolds was performed using Fourier transform infrared (FT-IR) spectroscopy analysis, swelling and scanning electron microscopy (SEM) tests. In vitro cytotoxicity assay with L929 cells and EpiDerm skin irritation test (SIT) using the EpiDerm reconstructed human epidermal (RHE) model were performed to analyze biocompatibility of the scaffolds. Characterization results showed that there were strong ionic interactions among chitosan, alginate and beta-TCP and the layers showed interconnected porous structure with different swelling ratios. The relative cell viability and SIT results were greater than 70% indicating that the scaffolds are considered nontoxic according to the International Organization for Standardization (ISO) standard. All results taken together, biomimetic TLS can be considered to be suitable for osteochondral applications.Öğe Energy drink induced lipid peroxidation and oxidative damage in rat liver and brain when used alone or combined with alcohol(Wiley, 2017) Reis, Rengin; Charehsaz, Mohammad; Sipahi, Hande; Doğan Ekici, Asiye Işın; Macit, Çağlar; Akkaya, Hatice; Aydın, AhmetEnergy drinks (ED) are containing large doses of metabolic stimulants and its use with ethanol has increased dramatically among young adults. In this study, we examined the effects of ED exposure either alone or in combination with ethanol on oxidative stress parameters including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and lipid peroxidation parameter malondialdehyde (MDA) in rat. Some histopathological findings were also evaluated. ED exposure led to a dose-dependent increase in liver MDA compared to the control indicating oxidative damage. Histopathological findings also revealed that ED alone may generate liver damage. Ethanol exposure increased MDA level and SOD, CAT, and GSH-Px activity in both the brain and the liver. The combination of ethanol and ED produced greater damage which is considered by further increases in SOD and GSH-Px activity in the brain. Similar results for MDA were observed in both the liver and brain as well. Our findings suggest that ED consumption alone or combination with ethanol may represent a significant public health concern.Öğe Evaluation of burn wound healing activity of novel fusidic acid loaded microemulsion based gel in male Wistar albino rats(Elsevier, 2020) Okur, Mehmet Evren; Ayla, Şule; Yozgatlı, Vildan; Aksu, Neşe Buket; Yoltaş, Ayşegül; Orak, Duygu; Sipahi, Hande; Üstünda? Okur, NeslihanThe objective of the present research was to examine the possible usage of microemulsion based gel for fusidic acid (FA) dermal application as burn wound treatment. During the preparation of microemulsion, ethyl oleate as oil phase, tween 80 as a surfactant, ethanol as co-surfactant, water as aqueous phase were used. The prepared microemulsions were evaluated for clarity, pH, viscosity and FA content. Moreover, stability, sterility, antibacterial activity, in vitro release of the formulations were also evaluated. The results showed that the FA loaded microemulsion and microemulsion based gel formation and characteristics were related to many parameters of the components. The performed optimized microemulsion-based gel showed good stability over a period of 3 months. The antibacterial activity of microemulsion-based gel was found to be comparable with marketed cream. RAW 264.7 macrophages were used to determine cell viability (MIT assay) and nitric oxide production. MBG and FA-MBG significantly inhibit the production of the inflammatory mediator NO in LPS-stimulated RAW 264.7 cells in a concentration-dependent manner. The wound healing property was evaluated by histopathological examination and by measuring the wound contraction. The % of wound area in rats treated with FA (2%) loaded microemulsion based gel ranged from 69.30% to 41.39% in the period from 3 to 10 days. In conclusion, FA loaded microemulsion based gel could be offered as encouraging strategy as dermal systems for the burn wound treatment.Öğe In vitro antibacterial, antioxidant, anti-inflammatory and analgesic evaluation of Rosmarinus officinalis L. flower extract fractions(Elsevier, 2019) Karadağ, Ayşe Esra; Demirci, Betül; Çaşkurlu, Ayşegül; Demirci, Fatih; Okur, Mehmet Evren; Orak, Deborah; Sipahi, Hande; Başer, Kemal Hüznü CanRosmarinus officinalis L. (rosemary) is a common culinary spice and herbal drug, which is used for centuries all over the world. In this present study, apolar to polar fractions of R. officinalis flowers were evaluated for their in vitro antioxidant, antibacterial, cytotoxic, anti-inflammatory and analgesic activities, respectively. Phytochemical compositions of R. officinalis extract fractions were analyzed by GC-MS and LC-MS. The antioxidant capacity of the fractions was evaluated by using the DPPH center dot and ABTS(center dot) methods. The antibacterial potential was determined using the in vitro broth microdilution assay against a panel of human pathogens. The analgesic and anti-inflammatory activities were investigated measuring nitric oxide (NO) and prostaglandin E-2 (PGE(2)) production in LPS-stimulated cells, respectively. In addition, in vitro cytotoxicity of the extract fractions was evaluated on RAW 264.7 murine macrophage cells by using the MTT assay. The constituents of the polar fractions were identified as rosmarinic acid, luteolin, quercetin and apigenin by LC techniques, whereas the n-hexane fraction was analyzed by GC-MS to determine the main volatile components camphor (19.6%), 1,8-cineole (11.7%), verbenone (11.5%), borneol (10.6%), alpha-pinene (5.8%), and linalool (5.7%). According to the bioactivity results, the polar fraction showed the highest antioxidant activity, whereas n-hexane fraction was found to be most effective against Staphylococcus aureus (78 mu g/mL). The n-hexane fraction (100 mu g/mL) reduced the LPS-induced NO and PGE(2) production capability. In conclusion, R. officinalis flower n-hexane and ethyl acetate fractions exhibited remarkable in vitro antibacterial, antioxidant, anti-inflammatory and analgesic activities possibly due to their polyphenol content, to the best of our knowledge for the first time.Öğe In vivo wound healing and in vitro anti-inflammatory activity evaluation of phlomis russeliana extract gel formulations(MDPI, 2020) Okur, Mehmet Evren; Karadağ, Ayşe Esra; Üstündağ Okur, Neslihan; Özhan, Yağmur; Sipahi, Hande; Ayla, Şule; Daylan, Benay; Demirci, Betül; Demirci, FatihThe air-dried aerial parts of Phlomis russeliana (Sims) Lag. Ex Benth. was extracted by methanol and fractionated by n-hexane, dichloromethane, and ethyl acetate, respectively. The wound healing properties of P. russeliana extract gel was evaluated using the in vivo excisional wound model using Balb-c mice. Initially, the P. russeliana methanol extract showed LOX inhibitory activity at IC50 = 23.2 mu g/mL, whereas the DPPH center dot assay showed IC50 = 0.89 mg/mL, and the ABTS(center dot) assay showed IC50 = 0.99 mg/mL, respectively. In addition, a remarkable anti-inflammatory activity was observed in the cell culture assay. Thereafter, activity-guided fractionation was performed by LOX enzyme inhibition assays, and the structures of the two most active fractions were revealed by both GC-FID and GC/MS analyses, simultaneously. Phytol and 1-heptadecanoic acid were characterized as the active constituents. Moreover, the P. russeliana extract gel formulation was applied for in vivo tests, where the new gel formulation supported the in vitro anti-inflammatory activity findings. As a conclusion, this experimental results support the wound healing evidence based on the ethnobotanical application of Phlomis species with further potential.Öğe New therapeutic system based on hydrogels for vaginal candidiasis management: Formulation-characterization andin vitroevaluation based on vaginal irritation and direct contact test(Taylor and Francis Ltd., 2020) Arpa, Muhammet Davut; Yoltaş, Ayşegül; Onay Tarlan, Ecehan; Şahin Şenyüz, Cemre; Sipahi, Hande; Aydın, Ahmet; Üstündağ Okur, NeslihanThe objective of the present research was to examine the possible usage of terbinafine loaded hydrogels for vaginal application as part of vaginal candidiasis treatment. Vaginal candidiasis belongs to the most frequent gynecological disorders. Various antifungal drugs are used for its treatment, with Terbinafine being one of them. In this study, new gel formulations were prepared for Terbinafine vaginal delivery. Natural polymers such as chitosan, sodium carboxymethylcellulose, and Carbopol were used for the development of Terbinafine vaginal gels. The developed gels were examined for their viscosity and spreadability, pH and mechanical properties. The most optimal formulations were further evaluated for theirin vitrorelease behavior and antifungal activities. In further, the cytotoxicity and irritation inducing capacity of optimum gel formulations were evaluated.In vitrodrug release studies demonstrated that terbinafine release was prolonged whereas anti-candida activity in several species showed the superiority of the gels compared to the marketed product. G-5 and G-8 gels did not cause lysis, hemorrhage and coagulation, therefore, classified as non-irritant. The optimal formulations were also studied for their stability, demonstrating that they were stable for 3 months.Öğe Novel benzofurane-pyrazole derivatives with anti-inflammatory, cyclooxygenase inhibitory and cytotoxicity evaluation(Walter de Gruyter GmbH, 2022) Şahin, Zafer; Özhan, Yağmur; Sipahi, Hande; Biltekin, Sevde Nur; Yurttaş, Leyla; Berk, Barkın; Demirayak, ŞerefNovel benzofurane-pyrazolone hybrids have been synthesized for evaluating their anti-inflammatory and cytotoxic properties. 4-(2-chloroacetyl)-1,5-dimethyl-2-phenyl-1,2-dihydro-3H-pyrazol-3-one were reacted with alpha-hydroxy aldehyde or alpha-hydroxy ketone derivatives to obtain nine novel pyrazolone derivatives. Structures were successfully elucidated by H-1 NMR, C-13 NMR, IR and HRMS. Enzyme inhibitory activity was measured on cyclooxygenases (COXs) as considered to address anti-inflammatory activity. Compound 2 showed the highest activity on both COX-1 and COX-2 subtypes with 12.0 mu M and 8.0 mu M IC50, respectively. This activity was found close to indomethacin COX-2 inhibition measured as 7.4 mu M IC50. Rest of the compounds (1, 3-9) showed 10.4-28.1 mu M IC50 on COX-2 and 17.0-35.6 mu M IC50 on COX-1 (Compound 1 has no activity on COX-1). Tested compounds (1-9) showed activity on NO production. Only compound was the 4, which showed a low inhibition on IL-6 levels. Cell viability was up to 60% at 100 mu M for all compounds (1-9) on RAW 264.7 and NIH3T3 cell lines, thus compounds were reported to be noncytotoxic.Öğe Novel cyanothiouracil and cyanothiocytosine derivatives as concentration-dependent selective inhibitors of U87MG glioblastomas: Adenosine receptor binding and potent PDE4 inhibition(Elsevier France-Editions Scientifiques Medicales Elsevier, 2021) Şahin, Zafer; Biltekin, Sevde Nur; Yurttaş, Leyla; Berk, Barkın; Özhan, Yağmur; Sipahi, Hande; Gao, Zhanguo; Jacobson, Kenneth A.; Demirayak, ŞerefThiouracil and thiocytosine are important heterocyclic pharmacophores having pharmacological diversity. Antitumor and antiviral activity is commonly associated with thiouracil and thiocytosine derivatives, which are well known fragments for adenosine receptor affinity with many associated pharmacological properties. In this respect, 33 novel compounds have been synthesized in two groups: 24 thiouracil derivatives (4a-x) and 9 thiocytosine derivatives (5a-i). Antitumor activity of all the compounds was determined in the U87 MG glioblastoma cell line. Compound 5e showed an anti-proliferative IC50 of 1.56 ?M, which is slightly higher activity than cisplatin (1.67 ?M). The 11 most active compounds showed no signficant binding to adenosine A1, A2A or A2B receptors at 1 ?M. Brain tumors express high amounts of phosphodiesterases. Compounds were tested for PDE4 inhibition, and 5e and 5f showed the best potency (5e: 3.42 ?M; 5f: 0.97 ?M). Remakably, those compounds were also the most active against U87MG. However, the compounds lacked a cytotoxic effect on the HEK293 healthy cell line, which encourages further investigation.Öğe Novel microemulsion containing benzocaine and fusidic acid simultaneously: Formulation, characterization, and in vitro evaluation for wound healing(Springer Science and Business Media Deutschland GmbH, 2024) Arpa, Muhammet Davut; Çağlar, Emre Şefik; Güreşçi, Dilara; Sipahi, Hande; Üstündağ Okur, NeslihanModern drug carrier technologies, such as microemulsions with small droplet sizes and high surface areas, improve the ability of low water solubility active ingredients to permeate and localize. The goal of this study was to create microemulsion formulations for wound healing that contained both fusidic acid (FA), an antibacterial agent, and benzocaine (BNZ), a local anesthetic. Studies on characterization were carried out, including viscosity, droplet size, and zeta potential. The drug-loaded microemulsion had a stable structure with –3.014 ± 1.265 mV of zeta potential and 19.388 ± 0.480 nm of droplet size. In both in vitro release and ex vivo permeability studies, the microemulsion was compared with Fucidin cream and oily BNZ solution. According to the drug release studies, BNZ release from the microemulsion and the BNZ solution showed a similar profile (p > 0.05), while FA release from the microemulsion had a higher drug release compared to Fucidin cream (p < 0.001). The microemulsion presented lower drug permeation (p > 0.05) for both active ingredients, on the other hand, provided higher drug accumulation compared to the control preparations. Moreover, according to the results of in vitro wound healing activity, the microemulsion indicated a dose-dependent wound healing potential with the highest wound healing activity at the highest concentrations. To the best of our knowledge, this developed BNZ- and FA-loaded microemulsion would be a promising candidate to create new opportunities for wound healing thanks to present the active ingredients, which have low water solubility, in a single formulation and achieved higher accumulation than control preparations.Öğe Phytochemical and in vitro pharmacological evaluation of Phlomis Pungens(Ankara University, 2022) Okur, Mehmet Evren; Karadağ, Ayşe Esra; Reis, Rengin; Sipahi, Hande; Arslan, Rana; Demirci, Betül; Demirci, FatihObjective: This study aimed to investigate the in vitro wound healing, anti-inflammatory, antimicrobial and antioxidant activity of Phlomis pungens Willd. extract derived from the aerial parts. Material and Method: The phytochemical analysis was performed using GC-MS in order to identify the volatile components of the bioactive Hex extract. The wound healing activity of P. pungens extract was evaluated based on in vitro antimicrobial, antioxidant, anti-inflammatory, and, scratch activity was studied. In addition, the in vitro cytotoxicity of the extract was also evaluated. Result and Discussion: P. pungens methanol extract depicted a 5-LOX inhibitory activity at 78.2µg/mL (IC50), while the antioxidant activity by DPPH radical provided an IC50=2.41mg/mL, and the ABTS radical showed IC50=3.32mg/mL, respectively. The extract showed dose-dependently anti-inflammatory activity while L-NAME and P. pungens methanol extract significantly decreased LPS stimulated PGE2 production. According to the scratch assay results, all treatments led to an increase in cell migration rate with a dose-dependent effect. Our findings suggested that P. pungens methanol extract may have a role in wound healing according to the scratch test, and it is thought that its antioxidant and anti-inflammatory activity also contributed. Further evaluations are ongoing to confirm the in vitro activity under in vivo conditions.Öğe Traditional herbal drugs against liver diseases – Experimented in vitro using HepG2 cells for induced steatosis(Marmara University, 2023) Karadağ, Ayşe Esra; Okur, Mehmet Evren; Özhan, Yağmur; Sipahi, Hande; Demirci, FatihSalvia fruticosa leaves, Malva sylvestris flowers, Taraxacum officinale aerial parts, Plantago ovata seeds, Tanacetum parthenium aerial parts, and Allium sativum bulbs are documented for traditional use against hepatic disorders and different liver diseases. To evaluate herbal drug material for potential use against liver diseases, at molecular level for the efficacy linked to ethnobotanical documented data.Different herbal extracts were prepared and standardized by HPLC, according to European Pharmacopoeia. Initially 0.25 mg/mL each standardized extract was applied to oleic+palmitic acid induced fatty liver using a HepG2 cell culture model. ALT, AST, GSH, and MDA levels were comparatively analyzed, in addition to cell Nil Red staining. The highest activity for MDA reduction was observed for the A. sativum extract at 48.2% level, followed by 36.4% for M. sylvestris, and S. fruticosa extracts with 27.3% reduction, respectively. Glutathione levels increased to 59.1% when A. sativum extract was applied. M. sylvestris extract increased the glutathione levels in the medium by 49.7%; S. fruticosa extract decreased ALT levels by 53.5% and M. sylvestris extract by 38.5%, whereas the standard resveratrol reduced ALT level by 30.9%, respectively. The AST levels for M. sylvestris extract was 46.5%, compared to resveratrol by 93%. A. sativum, M. sylvestris, and S. fruticosa standard extracts showed relatively good correlation and activity where further in vivo studies should be performed.Öğe Wound healing effect of naringin gel in alloxan induced diabetic mice(Ankara University Faculty of Pharmac, 2020) Okur, Mehmet Evren; Şakul, Ayşe Arzu; Ayla, Şule; Karadağ, Ayşe Esra; Şahin Şenyüz, Cemre; Batur, Şebnem; Daylan, Benay; Özdemir, Ekrem Musa; Palabıyık-Yücelik, Sezin; Sipahi, Hande; Aydın, AhmetObjective: The aim of this study was to investigate the effects of naringin, a known flavonoid, on in vitro cytotoxicity, irritation and in vivo potential efficacy when topically applied to the diabetic wound. Material and Method: In vitro direct contact assay and hen's egg chorio-allantoin membrane tests were used to evaluate irritation, and cytotoxicity potential of Naringin. In vitro antimicrobial activity was also tested. Topical treatments were administered once a day on the wound. Wound lesions were photographed and statistically analyzed. After the 10th day, histopathological parameters of tissues were assessed. Result and Discussion: The results showed that naringin gel has no cytotoxicity and irritation potential. Also, it displayed a weak antimicrobial effect. Aside from these, in vivo studies revealed that naringin gel showed statistically higher wound contraction and re-epithelialization, the thickness of granulation tissue, angiogenesis scores. The results indicated that naringin gel effectively enhanced diabetic wound healing, and so this formulation could be considered a promising therapeutic alternative for treating diabetic wounds.
