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Öğe 8-12 yaş arası çocuklarda görülen kötü ağız alışkanlıklarının sıklığı(Istanbul University Press, 2011) Yıldırım, Mine; Bayram, Merve; Patır, Aslı; Yalçın, Ferhan; Seymen, FigenBu çalışmanın amacı, 8-12 yaşları arasındaki çocuklarda kötü ağız alışkanlıklarının sıklığının değerlendirilmesi ve sonuçlarının yaşa ve ağız sağlığına göre karşılaştırılmasıdır. Yaşları 8 ile 12 arasında değişen 107 çocuk df, dfs, DMFT, DMFS, dental plak ve kanama indeksi, yanlış ağız hijyeni alışkanlıkları ve ağız solunumu, parmak emme, tırnak yeme, bebeklik yutkunması, bruksizm, emzik emme, dile baskı yapma, dudak ısırma, yanak ya da kalem ısırma, kendi kendine ağız dokularına zarar verme, kürdan kullanma gibi kötü ağız alışkanlıkları açısından değerlendirilmiştir. 59 çocuğun yüksek (%55.1), 19 çocuğun (%17.8) düşük çürük risk grubunda olduğu saptanmıştır. Düşük çürük risk grubundaki ortalama plak indeksi yüksek ve orta çürük risk grubuna göre istatistiksel olarak düşük bulunmuştur (p=0.031, p=0.022). Yaş ile kendi kendine ağız dokularına zarar verme arasında istatistiksel olarak anlamlı bir fark bulunmuştur (p=0.042). En sık görülen kötü ağız alışkanlığı 38 birey (%35.5) ile yanak veya kalem ısırma olarak saptanmıştır. Ağızdan solunum, tırnak yeme ve bebeklik yutkunması sırasıyla 20 (%18.7), 17 (%15.9), 12 (%11.2) çocukta saptanmıştır. Dile baskı yapma ile plak indeksi ve kürdan kullanma ile kanama indeksi arasında anlamlı bir farklılık bulunmuştur (p=0.013 p=0.01). Kötü ağız alışkanlıklarının dişhekimleri tarafından erken teşhisi ve uygun terapi yöntemlerinin uygulanması, bu tip alışkanlıkların durdurulup şiddetli anomalilerin önlenmesine yardımcı olacaktır.Öğe Alteration of exon definition causes amelogenesis imperfecta(SAGE Publications Inc., 2020) Kim, Young-jae; Kang, Jenny; Seymen, Figen; Koruyucu, Mine; Zhang, Hong; Kasımo?lu, Yelda; Bayram, Merve; Tuna-İnce, Elif Bahar; Bayrak, Şule; Tüloğlu, Nuray; Hu, Jan C.; Simmer, James P.; Kim, Jung-WookAmelogenesis imperfecta (AI) is a collection of genetic disorders affecting the quality and/or quantity of tooth enamel. More than 20 genes are, so far, known to be responsible for this condition. In this study, we recruited 3 Turkish families with hypomaturation AI. Whole-exome sequence analyses identified disease-causing mutations in each proband, and these mutations cosegregated with the AI phenotype in all recruited members of each family. The AI-causing mutations in family 1 were a novel AMELX mutation [NM_182680.1:c.143T>C, p.(Leu48Ser)] in the proband and a novel homozygous MMP20 mutation [NM_004771.3:c.616G>A, p.(Asp206Asn)] in the mother of the proband. Previously reported compound heterozygous MMP20 mutations [NM_004771.3:c.103A>C, p.(Arg35=) and c.389C>T, p.(Thr130Ile)] caused the AI in family 2 and family 3. Minigene splicing analyses revealed that the AMELX missense mutation increased exonic definition of exon 4 and the MMP20 synonymous mutation decreased exonic definition of exon 1. These mutations would trigger an alteration of exon usage during RNA splicing, causing the enamel malformations. These results broaden our understanding of molecular genetic pathology of tooth enamel formation.Öğe Aquaporin 5 interacts with fluoride and possibly protects against caries(Public Library of Science, 2015) Anjomshoaa, Ida; Briseno-Ruiz, Jessica; Deeley, Kathleen; Poletta, Fernardo; Mereb, Juan; Leite, Aline; Barreta, Priscila; Silva, Thelma; Dizak, Piper; Ruff, Timothy; Patır, Aslı; Koruyucu, Mine; Abbasoğlu, Zerrin; Casado, Priscila; Brown, Andrew; Zaky, Samer; Bayram, Merve; Kuechler, Erika; Cooper, Margaret; Liu, Kai; Marazita, Mary; Tanboğa, İlknur; Granjeiro, Jose; Seymen, Figen; Castilla, Eduardo; Orioli, Ieda; Sfeir, Charles; Owyang, Hongjiao; Buzalaf, Marilia; Vieira, AlexandreAquaporins (AQP) are water channel proteins and the genes coding for AQP2, AQP5, and AQP6 are clustered in 12q13. Since AQP5 is expressed in serous acinar cells of salivary glands, we investigated its involvement in caries. DNA samples from 1,383 individuals from six groups were studied. Genotypes of eight single nucleotide polymorphisms covering the aquaporin locus were tested for association with caries experience. Interaction with genes involved in enamel formation was tested. The association between enamel microhardness at baseline, after creation of artificial caries lesion, and after exposure to fluoride and the genetic markers in AQP5 was tested. Finally, AQP5 expression in human whole saliva, after exposure to fluoride in a mammary gland cell line, which is known to express AQP5, and in Wistar rats was also verified. Nominal associations were found between caries experience and markers in the AQP5 locus. Since these associations suggested that AQP5 may be inhibited by levels of fluoride in the drinking water that cause fluorosis, we showed that fluoride levels above optimal levels change AQP5 expression in humans, cell lines, and rats. We have shown that AQP5 is involved in the pathogenesis of caries and likely interacts with fluoride.Öğe Candidate gene studies in hypodontia suggest role for FGF3(2013) Vieira, Alexandre Rezende; D'Souza, Rena; Mues, Gabriele; Deeley, Kathleen; Hsin, Hongyuan; Küchler, Erika Calvano; Meira, Raquel; Patır, Aslı; Tannure, Patrícia Nivoloni; Lips, Andrea; Meneghim, Marcelo de Castro; Granjeiro, José Mauro; Seymen, Figen; Modesto, AdrianaIntroduction The majority of tooth agenesis cases are mild (hypodontia) and typically not associated with the gene mutations linked to oligodontia. From this, we hypothesise that most cases of tooth agenesis fit a polygenic mode of inheritance, where several genes with small effects cause a variety of varying phenotypes. Materials and methods In this study, we looked at 18 not typically studied genes in this condition, to ascertain their contribution to hypodontia. Our study subjects consisted of 167 patients with hypodontia and their parents from two cohorts (one from Brazil and one from Turkey). An additional 465 DNA samples (93 cases with hypodontia and 372 controls without family history for tooth agenesis or oral clefts) from Brazil were also available for this study. Ninety-three single nucleotide polymorphisms that maximally represent the linkage disequilibrium structure of the genes for the 18 genes were selected and genotyped using Taqman chemistry. Chi square was used to test if genotype distributions were in Hardy–Weinberg equilibrium, and 24 markers that were in Hardy–Weinberg equilibrium and had allele frequencies higher than 5 % in a panel of 50 CEPH samples were further tested. Association between hypodontia and genetic variants was tested with the transmission disequilibrium test within the programme Family-Based Association Test (FBAT) and by using Chi square and Fisher’s exact tests. Alpha at a level of 0.05 was used to report results.Öğe Clinical and oral findings of a patient with Simpson-Golabi-Behmel syndrome(Springer, 2015) Bayram, Merve; Yıldırım, Mine; Seymen, FigenBackground The Simpson-Golabi-Behmel syndrome (SGBS) is an overgrowth condition characterised by macrosomia, mental deficiency, large head, prominent skull sutures, midface deficiency, hypertelorism, broad nose, wide mouth, macroglossia, malocclusion, highly arched palate, and musculoskeletal and limb abnormalities. The aim of this case report is to present clinical and oral findings of an 8-year-old boy who had been diagnosed with SGBS. Case report This patient had supernumerary nipples on the right side, cubitus valgus webbed fingers, scoliosis, umbilical hernia, a coarse face, macrocephaly, hypertelorism, a short broad nose, a wide mouth, a straight facial profile and hearing loss. The patient also had macroglossia, diastemas, over-retained primary tooth, absent mandibular permanent central incisors, and highly arched palate. Lateral cephalometric analysis revealed a large anterior cranial base, a large maxilla and mandible, a large inferior face height, and skeletal Class III jaw relationship. Follow-up After extraction of the over-retained primary central tooth, a partial prosthesis was fabricated in order to maintain function. The patient has been recalled regularly at 6-month intervals for 2 years. Over the following years the prosthesis was replaced due to facial growth. Conclusion Long term follow-up is essential for the patient with SGBS. Preventive dental care, including oral hygiene instructions, diet counselling and the use of fluoride has been implemented.Öğe Comparison of root canal length measurement methods in primary teeth(University Library System, University of Pittsburgh, 2018) Koruyucu, Mine; Bayram, Merve; Kasımo?lu, Yelda; Seymen, FigenObjectves: The purpose of this study was to evaluate the accuracy of conven5onal radiography, intraoral digital radi- ovisiography and electronic apex locator in determining the working length of root canals in primary teeth (in-vivo) and to compare the results with scanning electron microscopy measurements (ex-vivo). Materials and Methods: This study was conducted on 50 primary molar teeth. Standard endodon7c access cavity prepara7ons were prepared and the actual length was calculated by calibrated inves3gators. Working lengths were determined by using conven5onal radiography, RVG and apex locator in a total of 116 root canals. A;er the extraconal radiog- raphy in determining the working length in primary teeth. Conclusions: The electronic apex locators provide an ac- ceptable level of accuracy in determining root canal length in primary teeth. Clinical Relevance: The purpose of this study was to evaluate the accuracy of conven,onal radiography, intraoral digital radiovisiography and electronic apex locator in determining the working length of root canals in primary teeth and,to compare the results with scanning electron microscopy measurements.Öğe Enamel formation genes influence enamel microhardness before and after cariogenic challenge(Public Library Science, 2012) Shimizu, Takehiko; Ho, Bao; Deeley, Kathleen; Briseno-Ruiz, Jessica; Faraco, Italo Medeiros; Schupack, Brett Ian; Brancher, Joäo Armando; Pecharki, Giovana Daniela; Küchler, Erika Calvano; Tannure, Patrícia Nivoloni; Lips, Andrea; Vieira, Thays Cristinedos Santos; Patır, Aslı; Yıldırım, Mine; Poletta, Fernando Adrián; Mereb, Juan Carlos; Resick, Judith M.; Brandon, Carla A.; Orioli, Ieda M.; Castilla, Eduardo Enrique; Marazita, Mary Louise; Seymen, Figen; Costa, Marcelo Costra; Granjeiro, José Mauro; Trevilatto, Paula Cristina; Vieira, Alexandre RezendeThere is evidence for a genetic component in caries susceptibility, and studies in humans have suggested that variation in enamel formation genes may contribute to caries. For the present study, we used DNA samples collected from 1,831 individuals from various population data sets. Single nucleotide polymorphism markers were genotyped in selected genes (ameloblastin, amelogenin, enamelin, tuftelin, and tuftelin interacting protein 11) that influence enamel formation. Allele and genotype frequencies were compared between groups with distinct caries experience. Associations with caries experience can be detected but they are not necessarily replicated in all population groups and the most expressive results was for a marker in AMELX (p = 0.0007). To help interpret these results, we evaluated if enamel microhardness changes under simulated cariogenic challenges are associated with genetic variations in these same genes. After creating an artificial caries lesion, associations could be seen between genetic variation in TUFT1 (p = 0.006) and TUIP11 (p = 0.0006) with enamel microhardness. Our results suggest that the influence of genetic variation of enamel formation genes may influence the dynamic interactions between the enamel surface and the oral cavity.Öğe Erken çocukluk döneminde travmatik diş yaralanmalarının, maloklüzyonların ve diş çürüğünün ağız sağlığı ile ilişkili yaşam kalitesi üzerine etkisi(Selcuk University, 2021) Gürcan, Aliye Tuğçe; Koruyucu, Mine; Bayram, Merve; Seymen, FigenAmaç: Bu çalışmanın amacı, travmatik diş yaralanmalarının (TDY) ve diş çürüğünün okul öncesi çocukların Ağız Sağlığı ile İlişkili Yaşam Kalitesi (OHRQoL) üzerindeki etkisini değerlendirmektir. Gereç ve Yöntemler: Ağız Sağlığı ile İlişkili Yaşam Kalitesini ölçmek için 206 okul öncesi çocuğun velisine 13 soruluk Erken Çocukluk Çağı Ağız Sağlığı Etki Ölçeği (ECOHIS) uygulanmıştır. Çocukların travmatik diş yaralanmaları, diş çürüğü (dmft skorları) ve ön bölge maloklüzyon özelliklerine bakılmıştır. Bulgular: Çocukların yaş ortalaması 4.09 ± 0.97 bulunmuştur. Diş çürüğü, Ağız Sağlığı ile İlişkili Yaşam Kalitesi üzerinde olumsuz bir etki göstermiştir. Diş çürüğü; Ağız Sağlığı ile İlişkili Yaşam Kalitesi ortalaması, semptomları, fonksiyonu, psikolojisi, ailesel sıkıntı ve aile fonksiyon alanları üzerinde olumsuz bir etki göstermiştir. Ön bölgede maloklüzyon varlığı sadece sosyal etkileşim alanı üzerinde olumsuz bir etki göstermiştir. Komplike travmatik diş yaralanmaları, komplike olmayan travmatik diş yaralanmalarına göre semptomlar açısından daha olumsuz bir etki göstermiştir. Sonuç: Travmatik dental yaralanma ve maloklüzyon gözlenen dişin varlığı, okul öncesi çocukların Ağız Sağlığı ile İlişkili Yaşam Kalitesi üzerinde yalnızca çocuk alanında olumsuz bir etkiye sahiptir; ancak, diş çürüğü, hem çocuk alanında hem aile alanında Ağız Sağlığı ile İlişkili Yaşam Kalitesi ile güçlü bir ilişkiye sahiptir.Öğe Estetik restoratif materyallerin cila işlemlerinden sonra yüzey yapısının sem ile incelenmesi(Atatürk Üniversitesi, 2012) Yıldırım, Mine; Patır, Aslı; Seymen, Figen; Gençay, KorayAmaç: Bu çalışmanın amacı, ışıkla polimerize olan iki farklı cam iyonomer materyali ile iki farklı posterior bölge kompozit materyalinin yüzey özelliklerini cila işlemleri uygulandıktan sonra analiz etmektir. Gereç-Yöntem: Boyutları 2mmX5mm olan silindir şeklindeki kalıplara her malzemeden 8’er örnek olacak şekilde tabakalama yöntemi ile numuneler yerleştirilmiş ve polimerizasyon sağlanmıştır (n:32). Aynı gün içerisinde numunelerin üzerine ayrı ayrı, sadece Sof-Lex disk (1), sadece cila lastikleri (2), Sof- Lex disk ve cila lastikleri (3), strip band (kontrol) (4) ile cila uygulanmıştır. Yüzey özellikleri SEM ile değerlendirilmiştir. Bulgular: Posterior bölge kompozit materyali olan Filtek Silorane, Filtek Supreme XT’den daha düzenli yüzey yapısı gösterirken, ışıkla polimerize olan cam iyonomerlerden Ketac Nano, Vitremerden daha düzenli yüzey yapısı göstermiştir. Düzenli yüzey yapısı, Filtrek Silorane için sırasıyla grup (3),(1),(2),(4) ile cilalandığında belirgin olarak görülmüştür. Ketac Nano için tüm gruplarda düzenli yüzey yapısı grup (3),(2),(4),(1) ile bitirme ve cila işlemi tamamlandığında görülmüştür. Sonuç: Kompozit materyallerinden Filtek Silorane, Filtek Supreme XT’den daha düzenli yüzey yapısı gösterirken, cam iyonomer materyallerinden Ketac Nano, Vitremerden daha düzenli yüzey yapısı göstermektedir. SEM analizlerine göre, en iyi cilalı yüzey Sof-lex disk ve ardından cila lastikleri kullanıldığında sağlanabilinmektedir.Öğe Fine mapping of locus Xq25.1-27-2 for a low caries experience phenotype(Pergamon-Elsevier Science Ltd., 2014) Küchler, Erika Calvano; Feng, Ping; Deeley, Kathleen; Fitzgerald, Carly A.; Meyer, Chelsea; Gorbunov, Anastasia; Bezamat, Mariana; Reis, Maria Fernanda; Noel, Jacqueline; Kouzbari, M. Zahir; Granjeiro, Jose Mauro; Antunes, Leonardo Santos; Antunes, Lívia Azeredo Alves; Abreu, Fernanda Volpe De; Meneghim, Marcelo de Castro; Tannure, Patrícia Nivoloni; Seymen, Figen; Koruyucu, Mine; Patır, Aslı; Vieira, Alexandre RezendeObjective: The purpose of this study was to fine map the locus Xq25.1-27-2 in order to identify genetic contributors involved in low caries experience. Design: Seventy-two families from the Philippines were studied. Caries experience was recorded and genomic DNA extracted from peripheral blood was obtained from all subjects. One hundred and twenty-eight polymorphisms in the locus Xq25.1-27-2, a region that contains 24 genes, were genotyped. Association between caries experience and alleles was tested using the transmission disequilibrium test (TOT). This initial analysis was followed by experiments with DNA samples from 1481 subjects from Pittsburgh, 918 children from Brazil, and 275 children from Turkey in order to follow up the results found in the Filipino families. Chi-square or Fisher's exact tests were used. Sequencing of the coding regions and exon-intron boundaries of MST4 and FGF13 were also performed on 91 women from Pittsburgh. Results: Statistically significant association with low caries experience was found for 11 markers in Xq25.1-27-2 in the Filipino families. One marker was in MST4, another marker was in FGF13, and the remaining markers were in intergenic regions. Haplotype analysis also confirmed these results, but the follow up studies with DNA samples from Pittsburgh, Brazil, and Turkey showed associations for a subset of the 11 markers. No coding mutations were identified by sequencing. Conclusions: Our study failed to conclusively demonstrate that genetic factors in Xq25.1-27-2 contribute to caries experience in multiple populations. (C) 2014 Elsevier Ltd. All rights reserved.Öğe Fine-Mapping of 5q12.1-13.3 unveils new genetic contributors to caries(Karger, 2013) Shimizu, Takehiko; Deeley, Kathleen; Briseno Ruiz, Jessica; Faraco, Italo Medeiros; Poletta, Fernando Adrian; Brancher, Joao Armando; Pecharki, Giovana Daniela; Kuechler, Erika Calvano; Tannure, Patricia Nivoloni; Lips, Andrea; Vieira, Thays Cristinedos Santos; Patır, Aslı; Yıldırım, Mine; Mereb, Juan Carlos; Resick, Judith; Brandon, Carla; Cooper, Margaret; Seymen, Figen; Costa, Marcelo de Castro; Granjeiro, Jose Mauro; Trevilatto, Paula Cristina; Orioli, Ieda Maria; Castilla, Eduardo Enrique; Marazita, Mary Louise; Vieira, Alexandre RezendeCaries is a multifactorial disease and little is still known about the host genetic factors influencing susceptibility. Our previous genome-wide linkage scan has identified the interval 5q12.1-5q13.3 as linked to low caries susceptibility in Filipino families. Here we fine-mapped this region in order to identify genetic contributors to caries susceptibility. Four hundred and seventy-seven subjects from 72 pedigrees with similar cultural and behavioral habits and limited access to dental care living in the Philippines were studied. DMFT scores and genotype data of 75 single-nucleotide polymorphisms were evaluated in the Filipino families with the Family-Based Association Test. For replication purposes, a total 1,467 independent subjects from five different populations were analyzed in a case-control format. In the Filipino cohort, statistically significant and borderline associations were found between low caries experience and four genes spanning 13 million base pairs (PART1, ZSWIM6, CCNB1, and BTF3). We were able to replicate these results in some of the populations studied. We detected PART1 and BTF3 expression in whole saliva, and the expression of BTF3 was associated with caries experience. Our results suggest BTF3 may have a functional role in protecting against caries.Öğe Gene-environment interaction in molar-incisor hypomineralization(Public Library of Science, 2021) Bezamat, Mariana; Souza, Juliana Feltrin de; Silva, Fernanda M.F.; Corrêa, Emilly G.; Fatturi, Aluhê Lopes; Brancher, Joäo Armando; Carvalho, Flávia M.; Cavallari, Tayla; Bertolazo, Laís; Machado-Souza, Cleber; Koruyucu, Mine; Bayram, Merve; Racic, Andrea; Harrison, Benjamin M.; Sweat, Yan Yan; Letra, Ariadne; Studen-Pavlovich, Deborah A.; Seymen, Figen; Amendt, Brad A.; Iani Werneck, Renata; Costa, Marcelo C.; Modesto, Adriana M.; Vieira, Alexandre RezendeMolar incisor hypomineralization (MIH) is an enamel condition characterized by lesions ranging in color from white to brown which present rapid caries progression, and mainly affects permanent first molars and incisors. These enamel defects usually occur when there are disturbances during the mineralization or maturation stage of amelogenesis. Both genetic and environmental factors have been suggested to play roles in MIH’s development, but no conclusive risk factors have shown the source of the disease. During head and neck development, the interferon regulatory factor 6 (IRF6) gene is involved in the structure formation of the oral and maxillofacial regions, and the transforming growth factor alpha (TGFA) is an essential cell regulator, acting during proliferation, differentiation, migration and apoptosis. In this present study, it was hypothesized that these genes interact and contribute to predisposition of MIH. Environmental factors affecting children that were 3 years of age or older were also hypothesized to play a role in the disease etiology. Those factors included respiratory issues, malnutrition, food intolerance, infection of any sort and medication intake. A total of 1,065 salivary samples from four different cohorts were obtained, and DNA was extracted from each sample and genotyped for nine different single nucleotide polymorphisms. Association tests and logistic regression implemented in PLINK were used for analyses. A potential interaction between TGFA rs930655 with all markers tested in the cohort from Turkey was identified. These interactions were not identified in the remaining cohorts. Associations (p<0.05) between the use of medication after three years of age and MIH were also found, suggesting that conditions acquired at the age children start to socialize might contribute to the development of MIH.Öğe Genetic influences on dental enamel that impact caries differ between the primary and permanent dentitions(Wiley, 2015) Bayram, Merve; Deeley, Kathleen; Reis, Maria; Trombetta, Vanessa; Ruff, Timothy; Sencak, Regina; Hummel, Michael; Dizak, Piper; Washam, Kelly; Romanos, Helena; Lips, Andrea; Alves, Gutemberg; Costa, Marcelo; Granjeiro, Jose; Antunes, Leonardo; Kuechler, Erika; Seymen, Figen; Vieira, AlexandreClinically, primary and permanent teeth are distinct anatomically and the presentation of caries lesions differs between the two dentitions. Hence, the possibility exists that genetic contributions to tooth formation of the two dentitions are different. The purpose of this study was to test the hypothesis that genetic associations with an artificial caries model will not be the same between primary and permanent dentitions. Enamel samples from primary and permanent teeth were tested for microhardness at baseline, after carious lesion creation, and after fluoride application to verify association with genetic variants of selected genes. Associations were found between genetic variants of ameloblastin, amelogenin, enamelin, tuftelin, tuftelin interactive protein 11, and matrix metallopeptidase 20 and enamel from permanent teeth but not with enamel from primary teeth. In conclusion, our data continue to support that genetic variation may impact enamel development and consequently individual caries susceptibility. These effects may be distinct between primary and permanent dentitions.Öğe Genetic mapping of high caries experience on human chromosome 13(BMC, 2013) Küchler, Erika Calvano; Deeley, Kathleen; Ho, Bao; Linkowski, Samantha; Meyer, Chelsea; Noel, Jacqueline; Kouzbari, M. Zahir; Bezamat, Mariana; Granjeiro, José Mauro; Antunes, Leonardo Santos; Antunes, Lívia Azeredo Alves; Abreu, Fernanda Volpe De; Meneghim, Marcelo de Castro; Tannure, Patrícia Nivoloni; Seymen, Figen; Koruyucu, Mine; Patır, Aslı; Mereb, Juan Carlos; Poletta, Fernando Adrián; Castilla, Eduardo Enrique; Orioli, Iêda Maria; Marazita, Mary Louise; Vieira, Alexandre RezendeBackground: Our previous genome-wide linkage scan mapped five loci for caries experience. The purpose of this study was to fine map one of these loci, the locus 13q31.1, in order to identify genetic contributors to caries. Methods: Seventy-two pedigrees from the Philippines were studied. Caries experience was recorded and DNA was extracted from blood samples obtained from all subjects. Sixty-one single nucleotide polymorphisms (SNPs) in 13q31.1 were genotyped. Association between caries experience and alleles was tested. We also studied 1,481 DNA samples obtained from saliva of subjects from the USA, 918 children from Brazil, and 275 children from Turkey, in order to follow up the results found in the Filipino families. We used the AliBaba2.1 software to determine if the nucleotide changes of the associated SNPs changed the prediction of the presence of transcription-binding site sequences and we also analyzed the gene expression of the genes selected based on binding predictions. Mutation analysis was also performed in 33 Filipino individuals of a segment of 13q31.1 that is highly conserved in mammals. Results: Statistically significant association with high caries experience was found for 11 markers in 13q31.1 in the Filipino families. Haplotype analysis also confirmed these results. In the populations used for follow-up purposes, associations were found between high caries experience and a subset of these markers. Regarding the prediction of the transcription-binding site, the base change of the SNP rs17074565 was found to change the predicted-binding of genes that could be involved in the pathogenesis of caries. When the sequence has the allele C of rs17074565, the potential transcription factors binding the sequence are GR and GATA1. When the subject carries the G allele of rs17074565, the potential transcription factor predicted to bind to the sequence is GATA3. The expression of GR in whole saliva was higher in individuals with low caries experience when compared to individuals with high caries experience (p = 0.046). No mutations were found in the highly conserved sequence. Conclusions: Genetic factors contributing to caries experience may exist in 13q31.1. The rs17074565 is located in an intergenic region and is predicted to disrupt the binding sites of two different transcription factors that might be involved with caries experience. GR expression in saliva may be a biomarker for caries risk and should be further explored.Öğe Genetic polymorphisms influence shear bond resistance of orthodontic brackets(Elsevier, 2020) Randazzo, Adam C.; Burnheimer, John M.; Bayram, Merve; Seymen, Figen; Vieira, Alexandre RezendeObjectives: The purpose of this study was to determine if shear bond resistance of orthodontic brackets bonded to enamel is associated with genes implicated in the enamel mineralization process.Methods: Ninety-two permanent, caries-free premolars extracted for orthodontic purposes and their associated saliva samples were obtained. Eighteen single nucleotide polymorphisms (SNPs) were studied for association with shear bond resistance. The genes of interest in this study were those previously associated with dental caries by our group. All tooth samples were bonded on the buccal surface with metallic lower lateral brackets, and then subjected to physical debonding. The force required to debond the bracket was recorded in Newtons (N) and converted to a shear bond resistance value in Megapascals (N/mm(2)). The data were analyzed for statistical significance as compared with the mean shear bond resistance value via PLINK whole genome analysis software.Results: Associations were found between the SNPs for tuftelin (rs7526319, P = 0.004) and amelogenin (rs17878486, P = 0.04) and a higher shear bond resistance.Conclusion: The collected data support the proposed hypothesis that genes involved in the mineralization process affect the bonding of orthodontic brackets, and such an association is of value for the field of orthodontics, particularly in evaluating the efficacy of enamel-resin bond strength for patients receiving treatment.Öğe In vitro acid-mediated initial dental enamel loss is associated with genetic variants previously linked to caries experience(Frontiers Media S.A., 2017) Vieira, Alexandre R.; Bayram, Merve; Seymen, Figen; Sencak, Regina C.; Lippert, Frank; Modesto, AdrianaWe have previously shown that AQP5 and BTF3 genetic variation and expression in whole saliva are associated with caries experience suggesting that these genes may have a functional role in protecting against caries. To further explore these results, we tested ex vivo if variants in these genes are associated with subclinical dental enamel mineral loss. DNA and enamel samples were obtained from 53 individuals. Enamel samples were analyzed for Knoop hardness of sound enamel, integrated mineral loss after subclinical carious lesion creation, and change in integrated mineral loss after remineralization. DNA samples were genotyped for single nucleotide polymorphisms using TaqMan chemistry. Chi-square and Fisher's exact tests were used to compare individuals above and below the mean sound enamel microhardness of the cohort with alpha of 0.05. The A allele of BTF3 rs6862039 appears to be associated with harder enamel at baseline (p = 0.09), enamel more resistant to demineralization (p = 0.01), and enamel that more efficiently regain mineral and remineralize (p = 0.04). Similarly, the G allele of AQP5 marker rs3759129 and A allele of AQP5 marker rs296763 are associated with enamel more resistant to demineralization (p = 0.03 and 0.05, respectively). AQP5 and BTF3 genetic variations influence the initial subclinical stages of caries lesion formation in the subsurface of enamel.Öğe Investigating the level of knowledge of the community about oral and dental health(Georg Thieme Verlag, 2021) Birant, Sinem; Koruyucu, Mine; Özcan, Hazal; İlisulu, Ceren; Kasımoğlu, Yelda; Üstün, Nilüfer; Kocaaydın, Simin; Bektaş, Dilara; Usta, Gülsevim; Akay Tekin, Cansu; Bekiroğlu, Nural; Seymen, FigenObjectives This study aimed to determine the knowledge and approaches of the participants about oral and dental health. Materials and Methods This was a questionnaire-based cross-sectional survey. Participants' demographic characteristics, tooth brushing habits, selection of toothpaste, and reasons for visiting the dentist were examined in this study. Participants' knowledge levels of fluoride and their attitudes toward the most common fluoride applications among preventive treatments were also evaluated. Data entry and analyses were performed using SPSS statistical software. Descriptive and inferential statistics and chi-square test were used for analyzes. Results A total of 2,744 voluntary participants including 1,938 (70.6%) females and 806 (29.4%) males responded; 1,391 (50.7%) participants know the contents of their toothpaste; 1,680 (61.2%) participants point out that fluoride is effective in preventing dental caries. Female participants reported a statistically significant difference in their knowledge of what is fluoride when compared with male participants (p = 0.0001). The knowledge level of participants who had higher levels of education were statistically significantly different when compared with the participants who had lower education levels about fluoride and fluoride applications (p = 0.0001). Conclusions The results of this study indicate that participants' attitudes toward oral health and dental care need to be improved.Öğe Investigation of surface topography of different root-end filling materials: an in vitro study(University Library System, University of Pittsburgh, 2019) Koruyucu, Mine; Özcan, Hazal; Bayram, Merve; Çankaya, Abdullah Burak; Kekliko?lu, Nurullah; Seymen, FigenAim: Although there are many materials that can be used for retrograde filling in surgical endodontics, none of them can be regarded as an ideal material yet. The purpose of this study was to compare the surface topography of three different root-end filling materials. Methods: 36 extracted single rooted human incisor teeth were cleaned and decoronated to standardized 10 mm root lengths. The root segments were prepared and 2 mm apical resection were performed. The samples were randomly separeted to three groups (Group A: Ca(OH)2, Group B: MTA Angelus, Group C: ProRoot MTA), each one of them was comprised of 12 roots. Materials were placed as 2 mm apical barriers and obturated with guttapercha and AH-Plus sealer. Each group was dimidiated into two subgroups (A1,A2,B1,B2,C1,C2). Groups A1,B1,C1 were stored in normal saline (NS), groups A2,B2,C2 were stored in neutral phosphate buffer saline (NPBS) solution and samples were incubated at 370C for 2 weeks. Stereomicroscope (32X) was used to photograph the root-end filling. Results: All specimens demonstrated white crystals formation and sediment over the root-end filling materials and on the superficial border of the root-end cavities’ wall as a white plaque. A2,B2,C2 samples had more crystal sediment on root-end fillings than samples A1,B1,C1. Dissolution and corrosion were observed in groups A1, A2. Conclusions: The results of this study revealed that calcium hydroxide is more resorbable than MTA Angelus and ProRoot MTA. The crystals formation and precipitation were observed in neutral phosphate buffer saline solution which were more than normal saline solution for all groups as a hydroxiapatite crystals.Öğe Mutations in RELT cause autosomal recessive amelogenesis imperfecta(Wiley, 2019) Kim, Jung-Wook; Zhang, Hong; Seymen, Figen; Koruyucu, Mine; Hu, Yuanyuan; Kang, Jenny; Kim, Youn Ju; Ikeda, Atsushi; Kasımoğlu, Yelda; Bayram, Merve; Zhang, Chuhua; Kawasaki, Kazuhiko; Bartlett, John D.; Saunders, Thomas L.; Simmer, James P.; Hu, Jan C-C.Amelogenesis imperfecta (AI) is a collection of isolated (non-syndromic) inherited diseases affecting dental enamel formation or a clinical phenotype in syndromic conditions. We characterized three consanguineous AI families with generalized irregular hypoplastic enamel with rapid attrition that perfectly segregated with homozygous defects in a novel gene: RELT that is a member of the tumor necrosis factor receptor superfamily (TNFRSF). RNAscope in situ hybridization of wild-type mouse molars and incisors showed specific Relt mRNA expression by secretory stage ameloblasts and by odontoblasts. Relt(-/-) mice generated by CRISPR/Cas9 exhibited incisor and molar enamel malformations. Relt(-/-) enamel had a rough surface and underwent rapid attrition. Normally unmineralized spaces in the deep enamel near the dentino-enamel junction (DEJ) were as highly mineralized as the adjacent enamel, which likely altered the mechanical properties of the DEJ. Phylogenetic analyses showed the existence of selective pressure on RELT gene outside of tooth development, indicating that the human condition may be syndromic, which possibly explains the history of small stature and severe childhood infections in two of the probands. Knowing a TNFRSF member is critical during the secretory stage of enamel formation advances our understanding of amelogenesis and improves our ability to diagnose human conditions featuring enamel malformations.Öğe Orthodontic treatment needs of children: Comparison of three index(Istanbul University, 2014) Koruyucu, Mine; İnce Tuna, Elif Bahar; Seymen, Figen; Münevveroğlu Patır, Aslı; Acar, GözdePurpose: An orthodontic treatment need index is a form of occlusal index devised initially to prioritize the need for treatment and to categorize the malocclusion and identify patients based upon treatment need. The aim of this study was to evaluate orthodontic treatment needs of children using IOTN (Index of Orthodontic Treatment Need), DAI (the Dental Aesthetic Index) and ICON (Index of Complexity, Outcome and Need) and the relationship among the three index. Material and Methods: After ethical approval and informed consent were obtained; 100 children aged 10-12 years were examined for malocclusion, overjet, overbite, open bite and crossbite. The study models of subjects were taken and the DAI, the Dental Health Component (DHC) and the Aesthetic Component (AC) of IOTN and ICON were compared. Statistical analysis was performed by using NCSS 2007 software and regression analysis was performed between the results. Results: The mean average scores of DAI, IOTN-DHC, IOTN-AC and ICON indices were 25.44±8.05, 2.46±0.98, 4.17±2.53 and 38.67±21.08, respectively. Statistically significant differences were found between DAI, IOTN-DHC, IOTN-AC and ICON scores (p<0.01). Conclusion: DAI, IOTN and ICON were found to be significantly correlated with each other regarding the prevalence of malocclusion and orthodontic treatment need.
