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  • Küçük Resim
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    Carvacrol alters soluble factors in HCT-116 and HT-29 cell lines
    (TUBITAK Scientific & Technical Research Council Turkey, 2020) Pakdemirli, Ahu; Karaca, Caner; Sever, Tolga; Daskın, Ezgi; Leblebici, Asım; Yiğitbaşı, Türkan; Başbınar, Yasemin
    Background/aim: Natural products are popular insights for researchers to investigate promising anti-cancer agents since some of these substances have lesser adverse effects restricting the treatment than traditional chemotherapeutic agents. A well-known monoterpene Carvacrol, widely consumed in Mediterranean cuisine and lower risks of cancer, has efficient anticancer effects. However, the mechanism of action is yet to be discovered.Materials and methods: The investigation aims to illuminate a new perceptive in the role of this substance on colorectal cancer treatment, by the means of differences in a well-defined range of soluble factors. Carvacrol effect on both HT-29 and HCT-116 cell lines was evaluated on proliferation and the IC 50 values were calculated by the RTCA xCELLigence device. Then MAGPIX assay was performed to obtain the changes in soluble factors of the cell lines.Results: The Multiplexing assay suggests some of these factors were altered in favor of surviving and proliferation in aggressive cell line HCT-116 whereas they were altered against these characters in HT-29, were correlated with the increased IC50 concentration of HCT116 in carvacrol treatment.Conclusion: The current study indicates that differences in the levels of these soluble factors could modulate the anticancer effect related to carvacrol.
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    Curcumin effects on cell proliferation, angiogenesis and metastasis in colorectal cancer
    (Zerbinis Publications, 2019) Çalıbaşı-Kocal, Gizem; Pakdemirli, Ahu; Bayrak, Serdar; Özüpek, Nazlı Mert; Sever, Tolga; Başbınar, Yasemin; Ellidokuz, Hülya; Yiğitbaşı, Türkan
    Purpose: Curcumin is a natural phytopolyphenol compound isolated from the root of turmeric (Curcuma longa) and possesses a wide range of biological properties. The purpose of this study was to evaluate the antiproliferative, wound healing, anti-invasive and anti-migrative effects of curcumin on HCT-116 and LoVo colorectal cancer cell lines. Methods: The antiproliferative activity of 2.5-75 µM curcumin was tested on HCT-116 and LoVo colorectal cell lines and the viability of the cells was tested with WST-1 reagent by using ELISA plate reader at 450 nm. xCELLigence RTCA DP system was used for the detection of anti-invasive and anti-migrative effects of curcumin. Results: The IC50 of curcumin was 10±0.03 for HCT-116 and 20±0.05 µM for LoVo cell lines. The IC50 of curcumin (10µM for HCT-116 and 20 µM for LoVo) showed anti-metastatic activity on these cell lines. Conclusion: This study showed that curcumin could be evaluated as a promising anti-cancer agent for human colorectal cancer.
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    Targeting the tumor metabolism by oxamate potentiates the impact of chemotherapeutics in colorectal cancer cells
    (Dokuz Eylül University Institute of Health Sciences, 2021) Çalıbaşı Koçal, Gizem; Çakıcı, Çağrı; Toksöz, Feriha; İnanç Sürer, Seniz; Sever, Tolga; Başbınar, Yasemin; Yiğitbaşı, Türkan
    Purpose: Cancer cells promote lactate formation via pyruvate rather than oxidative phosphorylation by programming their metabolism to maintain proliferation under the Warburg effect. It has shown that the altered metabolic phenotype with activation of lactate dehydrogenase-A in the cancer cell may affect survival, chemotherapy resistance, and metastasis. In this direction, studies are focusing on reprogramming cancer metabolism and increase the effectiveness of chemotherapy. In this study, the main aim was to target the Warburg phenotype via the inhibition of lactate dehydrogenase with the combination of sodium oxamate and current colorectal cancer treatment options such as 5-fluorouracil and irinotecan. Methods: The effect of chemotherapeutics on the cellular behavior was evaluated by real-time cytotoxicity and migration analysis systems, and metabolic phenotype was assessed by measuring lactate, lactate dehydrogenase expression, and reactive oxygen species levels. Results: According to the results, the viability and migration of colorectal cancer cells were significantly decreased with the combination of chemotherapeutics and sodium oxamate which decreases lactate levels. (C)onclusion: As a result, the combination of sodium oxamate with chemotherapeutics hinders the cancer cell viability and migration by changing metabolic phenotype with decreased lactate.