Yazar "Macit, Meltem" seçeneğine göre listele
Listeleniyor 1 - 4 / 4
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Chemical characterization and biological properties of oyster and shiitake mushrooms extracts and their liposomal formulations(2024) Macit, Çağlar; Eyüpoğlu, Ozan Emre; Macit, Meltem; Zengin, GökhanIn recent years, the production of liposomal formulations using mushroom extracts has gained increasing interest to increase the bioavailability of bioactive compounds in the nutraceutical field. Based on this information, we aimed to determine the antioxidant capacity (by DPPH, ABTS, CUPRAC and FRAP assays) and enzyme inhibition (against cholinesterase, amylase, glucosidase and tyrosinase) activities of alone and liposomal formulations of Pleurotus ostreatus (OYE) or Lentinus edodes (SHE) in different extracts solvents which were methanol (MeOH), aqua (Aq), methanol/aqua (MeOH/Aq). The extracts and formulations were chemically characterized using HPLC-DAD. The mean diameter of SL:SHE and SL:OYE (in MeOH/Aq) extended in range between 60 and 165 nm. The entrapment yields of SL:SHE and SL:OYE (in MeOH/Aq) were 63.8% ± 3.7% and 71.2% ± 2.8%, respectively. HPLC-DAD analysis revealed that ferulic and cinnamic acids were main components in the liposomal formulations. Liposomal formulations (in MeOH) showed higher antioxidant activity in the FRAP and CUPRAC assays. SL:SHE (in Aq) showed effective enzyme inhibition activity on acetylcholinesterase, tyrosinase, amylase and glucosidase enzymes. Based on our findings, the liposomal formulations can be a valuable strategy in preparing functional applications with shiitake and oyster mushrooms.Öğe Formulation development of liposomal coffee extracts and investigation of their antioxidant capacities(Elsevier, 2021) Macit, Meltem; Eyüpoğlu, Ozan Emre; Macit, Çağlar; Duman, Gülengülhe main objective of this study was to carry out the antioxidant capacity of a liposomal formulation either green coffee extract (GCE) and medium roasted coffee extract (RCE) in aq. and MeOH. First, the coffee beans were milled and medium roasted. The obtained green coffee (GC) and roasted coffee (RC) granules were also extracted into (both aq. and MeOH) (99%) solvents. The solvents were evaporated, and the dry extracts were freeze dried. Then, the soy lecithin (SL) (2% w/v) dispersion was prepared using microfludizer. The obtained SL dispersions were freeze dried by using lyophilizer and then, the GCE and RCE (in aq. and MeOH) dry extracts were incorporated into freeze dried liposomal SL dispersions as a one to fourth portion. The liposomal formulations were titled as SL: GCE and SL: RCE, respectively. The SL: GCE and SL: RCE (either in aq. or MeOH) were characterized and evaluated by dynamic light scattering (DLS) technique. Additionally, the antioxidant capacity of all liposomal formulations was performed using by three different analysis methods; 1,1-diphenyl-2-picrylhydrazyl Radical (DPPH.) Scavenging Activity, Cupric Ion Reducing Antioxidant Capacity (CUPRAC) and Ferric Reducing Antioxidant Power (FRAP), respectively. Moreover, HPLC analysis was performed and phenolic compound such as caffeine, chlorogenic acid, ferulic acid, protocatechuic acid, caffeic acid and rosmarinic acid were evaluated. Finally, the SL: RCE (in MeOH) was formulated as an edible film using solvent casting method. The SL: RCE (in MeOH) extract was incorporated into pullulan (20% w/w) solution. Next, the film was made by using oven at 50 °C and 65% relative humidity (RH). DLS results showed mean diameter of liposomal formulations of SL: RCE and SL: GCE (in MeOH) ranged between 110 and 500 nm. The polydispersity index (PDI) were found around 0.3 and 0.6, respectively. Finally, their average zeta-potential values were obtained ?40 and ?70 mV. It was found that both of the SL: GCE and SL: RCE (in aq. and MeOH) almost twice as much as higher antioxidant capacity then alone GC and RC (in aq. and MeOH) (p < 0.05). According to HPLC results, nanoforms of methanolic coffee extracts (SL: GCE and SL: RCE) had the highest caffeine (2.21%, 2.2%) and chlorogenic acid (1.81%, 0.48%) contents, respectively. These findings have proven to liposomal coffee extract formulation has a strong antioxidant capacity. The final formulation of an edible film of SL: RCE (in MeOH) will be evaluated according to antioxidant capacity as a future work.Öğe Formulation development of Silybum marianum seed extracts and silymarin nanoparticles, and evaluation of hepatoprotective effect(Editions de Sante, 2023) Macit, Meltem; Duman, Gülengül; Cumbul, Alev; Sümer, Engin; Macit, ÇağlarIn this study, we formulated silymarin-nanoparticles and assayed their ability to reduce acetaminophen-induced toxicity in vitro and in vivo. After silymarin molecules were encapsulated the loading efficiency and the physicochemical properties of fabricated nanoparticles were investigated by HPLC and DLS analysis. The in vivo hepatoprotective studies were conducted on rats in an optimized setting. The nanoformulation was presented a significant (p ? 0.05) hepatoprotective effect by reducing the serum marker enzymes such as AST, and ALT. The histopathological study further confirmed the hepatoprotective activity of the nanoformulations when compared with the acetaminophen-induced, two different silymarin formulations as treatment group and control group. These results indicate that the nano approaches could be used to improve the therapeutic efficacy of the nano-silymarin. The formulation of silymarin-nanoparticles showed a spherical shape with an average diameter between 138.9 nm and 1155 nm, the zeta potential of – 0.0340 mV. The average loading efficiency was found around 32 ± 0.5%.Öğe Steam bath, vibration, and thermal ablation administrations augment the release of tramadol HCl from transdermal patch and enhance the plasma concentration in rats(Marmara University, 2023) Macit, Çağlar; Duman, Gülengül; Macit, MeltemRecently, transdermal drug delivery has become popular due to their numerous advantages. They offer non-invasive application and eliminate the first-pass metabolism. The skin membrane is sensitive to heat and vibration that these applications enhance the skin permeability resulting in increased bioavailability. This study aims to determine the effect of steam bath (STB), vibration (VIB) and thermal ablation (THAB) on systemic absorption of tramadol HCl and compare all applications with each other. After preparing of tramadol HCl patches, in vitro release tests followed by in vivo animal experiments were conducted. The patches were applied to 32 Wistar albino rats divided into four groups: No potential trigger effect (NPTE), STB, VIB, and THAB. STB, VIB and THAB were applied by purchased devices. One hour later, the patches were removed and plasma concentration of tramadol HCl was measured by UV-Vis spectrophotometry at 271 nm. When compared to the control group, calculated plasma tramadol HCl µg/mL concentration increased significantly in STB, VIB and THAB (p<0.001). Finally, as trigger effects, steam bath, vibration and thermal ablation (42°C) dramatically increased the absorption amount of tramadol HCl (42.1%, 37.2 % and 43.8 %, respectively). The percentage release of tramadol HCl increased significantly in investigation groups when compared to NPTE (p[removed]
