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  • Küçük Resim
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    Carvacrol alters soluble factors in HCT-116 and HT-29 cell lines
    (TUBITAK Scientific & Technical Research Council Turkey, 2020) Pakdemirli, Ahu; Karaca, Caner; Sever, Tolga; Daskın, Ezgi; Leblebici, Asım; Yiğitbaşı, Türkan; Başbınar, Yasemin
    Background/aim: Natural products are popular insights for researchers to investigate promising anti-cancer agents since some of these substances have lesser adverse effects restricting the treatment than traditional chemotherapeutic agents. A well-known monoterpene Carvacrol, widely consumed in Mediterranean cuisine and lower risks of cancer, has efficient anticancer effects. However, the mechanism of action is yet to be discovered.Materials and methods: The investigation aims to illuminate a new perceptive in the role of this substance on colorectal cancer treatment, by the means of differences in a well-defined range of soluble factors. Carvacrol effect on both HT-29 and HCT-116 cell lines was evaluated on proliferation and the IC 50 values were calculated by the RTCA xCELLigence device. Then MAGPIX assay was performed to obtain the changes in soluble factors of the cell lines.Results: The Multiplexing assay suggests some of these factors were altered in favor of surviving and proliferation in aggressive cell line HCT-116 whereas they were altered against these characters in HT-29, were correlated with the increased IC50 concentration of HCT116 in carvacrol treatment.Conclusion: The current study indicates that differences in the levels of these soluble factors could modulate the anticancer effect related to carvacrol.
  • Küçük Resim
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    Limitations of cross-talk between osteosarcoma and bone marrow-derived mesenchymal stem cells
    (Aves Press Ltd., 2019) Başbınar, Yasemin; Uysal, Tuğba; Karaca, Caner; Daşkın, Ezgi; Meco, Hanife Ecenur; Pakdemirli, Ahu; Yiğitbaşı, Türkan; Ellidokuz, Hülya
    Objectives: Metastasis is a multi-step process which leads the tumor cells to escape from primer tumor region due to their need to gain malign phenotypes. While the effect of bone marrow-derived mesenchymal stem cells upon metastasis is not certain, some studies point out bone marrow-derived mesenchymal stem cells (BM-MSCs) to have this ability due to cell-cell interaction, released cytokines, and organization with the extracellular matrix in the micro-environment. Cross-talk via soluble factors also shifts the metastatic character. Patients and Methods: In this study, the effects of mesenchymal stem cells on tumor behavior by creating different microenvironments in 3-dimensional (3D) in vitro cancer model is analyzed. The BM-MSCs and osteosarcoma cells were co-cultured via hanging-drop modeled 3D structure in normoxic and hypoxic conditions, and the cross-talk was modeled to measure their chemoattractant effects The invasion and migration rates were measured with xCELLigence DP real-time cell analysis system. Mann Whitney U Test was used to compare independent samples. All P-values <0.05 were considered statistically significant. Results: In this study, the most effective chemoattractant that increases the rate of migration in the osteosarcoma cell line under both normoxic (P 0.02) and hypoxic (P 0.004) conditions have been found to be the chemoattractant obtained from the BMSC culture. Conclusion: Soluble factors secreted by BM- MSCs to micro-environment are highly effective chemoattractants for osteosarcoma, nevertheless the stem cells that have been co-cultured with the MG-63 decrease this behavior. These results could provide a new scientific approach to downregulate the metastasis induced by the effect of BM-MSCs.