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    Effect of different final irrigation solutions on dentinal tubule penetration depth and percentage of root canal sealer
    (Elsevier, 2012) Kara-Tuncer, Aysun; Tuncer, Safa
    Introduction: The purpose of this study was to evaluate the effects of different solutions used for final irrigation on sealer penetration into dentinal tubules. Methods: Thirty-two recently extracted human mandibular premolar teeth were treated with sodium hypochlorite (NaOCl) irrigation. The samples were divided into 4 groups according to the final irrigation solution used: (1) the EDTA group: 17% EDTA + 2.5% NaOCl, (2) the maleic acid (MA) group: 7% MA + 2.5% NaOCl, (3) the citric acid (CA) group: 10% CA + 2.5% NaOCl, and (4) the control group: 2.5% NaOCl. All teeth were obturated using the cold lateral condensation technique with gutta-percha and AH 26 sealer (Dentsply; DeTrey, Konstanz, Germany) labeled with fluorescent dye. The teeth were sectioned at distances of 2, 5, and 8 mm from the root apex. Total percentage and maximum depth of sealer penetration were measured using confocal laser scanning microscopy. Results: The Kruskal-Wallis analysis results showed that there was a significant difference in the percentage and maximum depth of sealer penetration among all groups in all sections (P<.05). The coronal sections in each group showed a significantly higher percentage and maximum depth of sealer penetration than did the apical and middle sections (P<.05). Conclusions: Final irrigation with EDTA, MA, and CA after the use of NaOCl affected sealer penetration. However, there was no significant difference between these experimental groups (EDTA, MA, and CA) in all sections.
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    Inhibition of cell survival, viability and proliferation by dentin adhesives after direct and indirect exposure in vitro
    (Springer, 2012) Tuncer, Safa; Demirci, Mustafa; Schweikl, Helmut; Ergüven, Mine; Bilir, Ayhan; Kara-Tuncer, Aysun
    Objectives The influence of dentin adhesive systems (Scotchbond Multi-Purpose, XP Bond, Xeno V, Clearfil Protect Bond, AdheSE) on cell survival, viability and proliferation was characterized after direct and indirect exposure using different cell culture techniques. Materials and methods The primers and cured bonding parts were directly exposed to cells using cell culture inserts, and complete materials were analyzed in a dentin barrier test. Cell responses were examined in 3T3 mouse fibroblasts after 24- and 72-h exposure periods by the estimation of total cell numbers (survival), apoptosis (viability) and cell proliferation. Results Cell numbers were effectively reduced by the primers of AdheSE, Protect Bond, and Scotchbond Multi-Purpose as well as XP bond after direct exposure in a cell culture insert test device. Likewise, Scotchbond Multi-Purpose primer induced a rate of apoptosis (93.9%) even higher than detected with Protect Bond primer (91.6%). Cell proliferation was entirely inhibited by primers and by Xp Bond as well. The Scotchbond Multi-Purpose was most cytotoxic in a dentin barrier test device after a 24-h indirect exposure. It also increased the percentage of cells in apoptosis to 15.4% compared to untreated controls. Conclusion Unpolymerized primers of dentin adhesives were more cytotoxic than polymerized bonding counterparts. Moreover, total etch dentin adhesives were more cytotoxic than self-etch adhesives. Clinical relevance When dentin adhesives are used in deep cavities without a protective dentin barrier the leachable hydrophobic and hydrophilic component of dentin adhesive systems can penetrate to the pulp and may induce cytotoxic responses in pulp tissues.