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    Exploiting stokes and anti-stokes type emission profiles of aptamer-functionalized luminescent nanoprobes for multiplex sensing applications
    (Wiley-VCH Verlag Gmbh, 2018) Yüce, Meral; Kurt, Hasan; Hussain, Babar; Ow-Yang, Cleva W.; Budak, Hikmet
    Multiplex biosensing of four types of bacterial food pathogens in one pot is described. Stokes and anti-Stokes type photoluminescence (PL) of two quantum dots (QD) and two upconverting nanoparticles (UCNP) were utilised for realisation of the multiplex detection. The biosensing system was composed of aptamer-functionalized QD and UCNP nanoprobes that were conjugated with partially complementary DNA-modified magnetic beads for separation. PL signals of the conjugates were collected before and after incubation with target pathogens in one pot through the sequential excitations at 335nm for QD probes and 980nm for UCNP probes. The limit of detection values achieved were 28, 15, 12 and 25 cfu center dot mL(-1) for Listeria monocytogenes, Staphylococcus aureus, Salmonella typhimurium and Pseudomonas aeruginosa, respectively. Efforts for extending the multiplex detection up to five pathogens were also presented even though the PL signal cross-talk of a third QD nanoprobe hindered the detection. This work empowers co-deployment of QDs and UCNPs and paves the way for future studies in multiplex sensing, labelling, and bioimaging fields.
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    Systematic evolution of ligands by exponential enrichment for aptamer selection
    (Elsevier, 2018) Yüce, Meral; Kurt, Hasan; Hussain, Babar; Budak, Hikmet
    Discovery of aptamers and developments in the field of nanotechnology has made a significant contribution to the field of biosensing. Nanomaterials with various physical, optic, and electronic properties have been functionalized with aptamers, which are the synthetic counterparts of the conventional antibodies, to be used as more stable and cost-effective affinity probes for the detection of target molecules ranging from small ions, disease biomarkers, cell surfaces, microorganisms, and hazardous materials to food-borne allergens and pathogens. In this chapter, we described the fundamental methodology for in vitro aptamer selection, known as systematic evolution of ligands by exponential enrichment, with recent examples from the literature. We also provide the audience with graphical abstracts and illustrations for a better understanding of the aptamer selection concept.