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  • Küçük Resim
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    Effects of systemic erythropoietin treatment and heterogeneous xenograft in combination on bone regeneration of a critical-size defect in an experimental model
    (Churchill Livingstone, 2018) Diker, Nurettin Yağmur; Sarıcan, Hande; Cumbul, Alev; Kılıç, Ertuğrul
    The aim of the present study was to evaluate the effects of systemic EPO treatment alone or in combination with xenogenic bone graft augmentation on bone regeneration. Eleven adult male Sprague-Dawley rats were used in the present study. Rats were subjected to bilateral 5 mm critical size bone defects on the parietal bones under general anaesthesia. Right parietal bone defects were augmented with xenogenic bone graft and left parietal bone defect was left empty. Rats were randomly assigned for one of the two groups. One group of rats received (i) vehicle (n = 6) and other group received (ii) EPO (500IU kg/day) (n = 5). EPO treatment was continued for 28 days. Vascularization was analysed by immunohistochemical staining of CD31 (PECAM-1) and new bone formation was histomorphometrically evaluated. Xenogenic graft augmentation enhanced bone formation and vascularization significantly in either vehicle or EPO treated groups (p <0.05). Histomorphometric results of angiogenesis was similar in the EPO treated group and the control group. However, angiogenesis was significantly higher in the combination of systemic EPO treatment with graft augmentation than graft augmentation alone (p <0.01). Graft augmentation for treatment of critical size bone defects seems essential for proper bone healing. Results of the present study suggest that EPO potentiates the regenerative processes of augmented bone defects.
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    Formulation development of Silybum marianum seed extracts and silymarin nanoparticles, and evaluation of hepatoprotective effect
    (Editions de Sante, 2023) Macit, Meltem; Duman, Gülengül; Cumbul, Alev; Sümer, Engin; Macit, Çağlar
    In this study, we formulated silymarin-nanoparticles and assayed their ability to reduce acetaminophen-induced toxicity in vitro and in vivo. After silymarin molecules were encapsulated the loading efficiency and the physicochemical properties of fabricated nanoparticles were investigated by HPLC and DLS analysis. The in vivo hepatoprotective studies were conducted on rats in an optimized setting. The nanoformulation was presented a significant (p ? 0.05) hepatoprotective effect by reducing the serum marker enzymes such as AST, and ALT. The histopathological study further confirmed the hepatoprotective activity of the nanoformulations when compared with the acetaminophen-induced, two different silymarin formulations as treatment group and control group. These results indicate that the nano approaches could be used to improve the therapeutic efficacy of the nano-silymarin. The formulation of silymarin-nanoparticles showed a spherical shape with an average diameter between 138.9 nm and 1155 nm, the zeta potential of – 0.0340 mV. The average loading efficiency was found around 32 ± 0.5%.
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    Sodium pentaborate pentahydrate and pluronic containing hydrogel increases cutaneous wound healing in vitro and in vivo
    (Humana Press, 2014) Do?an, Ayşegül; Demirci, Selami; Çağlayan, Ahmet Burak; Kılıç, Ertuğrul; Günal, Mehmet Yalçın; Uslu, Ünal; Cumbul, Alev; Şahin, Fikrettin
    After a disruption of skin integrity, the body produces an immediate response followed by a functional and comparable regeneration period, referred to as wound healing. Although normal wounds do not need much attention during the healing period, chronic (non-healing) wounds are the major challenge of current dermatological applications. Therefore, developing new, safe, and effective wound healing drugs has always been an attractive area of international research. In the current study, sodium pentaborate pentahydrate (NaB), pluronics (Plu; F68 and F127), and their combinations were investigated for their wound healing activities, using in vitro and in vivo approaches. The results revealed that NaB significantly increased migration capacity and superoxide dismutase activity in primary human fibroblasts. Combinations of optimized concentrations for pluronic block co-polymers further increased cell migration, and the messenger RNA (mRNA) expression levels of important growth factor and cytokines (vascular endothelial growth factor (VEGF), transforming growth factor beta (TGF-ß), and tumor necrosis factor alpha (TNF-?)). NaB containing hydrogel co-formulated with pluronics was also investigated for their wound healing activities using a full thickness wound model in rats. Macroscopic and histopathological analysis confirmed that wounds in combination gel-treated groups healed faster than those of control groups. NaB/Plu gel application was found to increase wound contraction and collagen deposition in the wound area. Therefore, our results suggest that NaB, and its pluronics combination, could be used in dermatological clinics and be a future solution for chronic wounds. However, further studies should be conducted to explore its exact action of mechanism and effects of this formulation on chronic wounds.