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Öğe 8-12 yaş arası çocuklarda görülen kötü ağız alışkanlıklarının sıklığı(Istanbul University Press, 2011) Yıldırım, Mine; Bayram, Merve; Patır, Aslı; Yalçın, Ferhan; Seymen, FigenBu çalışmanın amacı, 8-12 yaşları arasındaki çocuklarda kötü ağız alışkanlıklarının sıklığının değerlendirilmesi ve sonuçlarının yaşa ve ağız sağlığına göre karşılaştırılmasıdır. Yaşları 8 ile 12 arasında değişen 107 çocuk df, dfs, DMFT, DMFS, dental plak ve kanama indeksi, yanlış ağız hijyeni alışkanlıkları ve ağız solunumu, parmak emme, tırnak yeme, bebeklik yutkunması, bruksizm, emzik emme, dile baskı yapma, dudak ısırma, yanak ya da kalem ısırma, kendi kendine ağız dokularına zarar verme, kürdan kullanma gibi kötü ağız alışkanlıkları açısından değerlendirilmiştir. 59 çocuğun yüksek (%55.1), 19 çocuğun (%17.8) düşük çürük risk grubunda olduğu saptanmıştır. Düşük çürük risk grubundaki ortalama plak indeksi yüksek ve orta çürük risk grubuna göre istatistiksel olarak düşük bulunmuştur (p=0.031, p=0.022). Yaş ile kendi kendine ağız dokularına zarar verme arasında istatistiksel olarak anlamlı bir fark bulunmuştur (p=0.042). En sık görülen kötü ağız alışkanlığı 38 birey (%35.5) ile yanak veya kalem ısırma olarak saptanmıştır. Ağızdan solunum, tırnak yeme ve bebeklik yutkunması sırasıyla 20 (%18.7), 17 (%15.9), 12 (%11.2) çocukta saptanmıştır. Dile baskı yapma ile plak indeksi ve kürdan kullanma ile kanama indeksi arasında anlamlı bir farklılık bulunmuştur (p=0.013 p=0.01). Kötü ağız alışkanlıklarının dişhekimleri tarafından erken teşhisi ve uygun terapi yöntemlerinin uygulanması, bu tip alışkanlıkların durdurulup şiddetli anomalilerin önlenmesine yardımcı olacaktır.Öğe Alteration of exon definition causes amelogenesis imperfecta(SAGE Publications Inc., 2020) Kim, Young-jae; Kang, Jenny; Seymen, Figen; Koruyucu, Mine; Zhang, Hong; Kasımo?lu, Yelda; Bayram, Merve; Tuna-İnce, Elif Bahar; Bayrak, Şule; Tüloğlu, Nuray; Hu, Jan C.; Simmer, James P.; Kim, Jung-WookAmelogenesis imperfecta (AI) is a collection of genetic disorders affecting the quality and/or quantity of tooth enamel. More than 20 genes are, so far, known to be responsible for this condition. In this study, we recruited 3 Turkish families with hypomaturation AI. Whole-exome sequence analyses identified disease-causing mutations in each proband, and these mutations cosegregated with the AI phenotype in all recruited members of each family. The AI-causing mutations in family 1 were a novel AMELX mutation [NM_182680.1:c.143T>C, p.(Leu48Ser)] in the proband and a novel homozygous MMP20 mutation [NM_004771.3:c.616G>A, p.(Asp206Asn)] in the mother of the proband. Previously reported compound heterozygous MMP20 mutations [NM_004771.3:c.103A>C, p.(Arg35=) and c.389C>T, p.(Thr130Ile)] caused the AI in family 2 and family 3. Minigene splicing analyses revealed that the AMELX missense mutation increased exonic definition of exon 4 and the MMP20 synonymous mutation decreased exonic definition of exon 1. These mutations would trigger an alteration of exon usage during RNA splicing, causing the enamel malformations. These results broaden our understanding of molecular genetic pathology of tooth enamel formation.Öğe Analysis of the involvement of the thyroid gland using computed tomography in patients with suspected SARS-CoV-2 infection: A retrospective study(Verduci Editore s.r.l, 2021) Akkoyunlu, Yasemin; Kalaycıoğlu, Bora; Yurtsever, İsmail; Eren, Abdulkadir; Bayram, Merve; Mert, Ali; Akkoyunlu, Muhammed EminOBJECTIVE: SARS-CoV-2 primarily infects the respiratory tract and leads to severe pneumonia by binding to the ACE-2 receptor. The virus can also interact with ACE-2 receptors expressed in other tissues as in thyroid. This study predicted the complications involving the thyroid in patients with suspected SA RS-CoV-2. PATIENTS AND METHODS: Patients with suspected SARS-CoV-2 infection between March 11, 2020-May 31, 2020 were retrospectively evaluated. Sixty-nine patients who were radiologically diagnosed as COVID-19 according to thoracic CT and had previously performed thoracic CT before November 2019 were included in the study according to the exclusion and inclusion criteria. Age and gender-matched controls (No. 69) were selected with normal thoracic CT whose PCR tests were also negative. Thyroid densities of participants were calculated and compared from the previous and current thoracic CTs. Results were also compared with the control group. RESULTS: Participants were composed of 69 patients (39 male, mean age 64.35 years). Thyroid densities were significantly decreased from 89HU to 76HU for whole gland, from 88HU to 76HU for right lobes and from 87.5HU to 75.5HU for left lobes at current thoracic CTs performed during COVID-19 (p<0.001, p<0.001, p<0.001 respectively). The decrease in densities of the whole thyroid gland. both left and right lobes. was correlated with mortality (p<0.001). The changes in thyroid densities were not correlated with age nor gender. The decreases in HU values of thyroid densities for whole gland, left and right lobes, were correlated with mortality (p<0.001, p<0.001. and p<0.001 respectively). CONCLUSIONS: COVID-19 is a multi-systemic disease that threatens vital organs, including the thyroid. Future studies are needed to investigate the association between SARS-CoV-2 and other complications.Öğe Aquaporin 5 interacts with fluoride and possibly protects against caries(Public Library of Science, 2015) Anjomshoaa, Ida; Briseno-Ruiz, Jessica; Deeley, Kathleen; Poletta, Fernardo; Mereb, Juan; Leite, Aline; Barreta, Priscila; Silva, Thelma; Dizak, Piper; Ruff, Timothy; Patır, Aslı; Koruyucu, Mine; Abbasoğlu, Zerrin; Casado, Priscila; Brown, Andrew; Zaky, Samer; Bayram, Merve; Kuechler, Erika; Cooper, Margaret; Liu, Kai; Marazita, Mary; Tanboğa, İlknur; Granjeiro, Jose; Seymen, Figen; Castilla, Eduardo; Orioli, Ieda; Sfeir, Charles; Owyang, Hongjiao; Buzalaf, Marilia; Vieira, AlexandreAquaporins (AQP) are water channel proteins and the genes coding for AQP2, AQP5, and AQP6 are clustered in 12q13. Since AQP5 is expressed in serous acinar cells of salivary glands, we investigated its involvement in caries. DNA samples from 1,383 individuals from six groups were studied. Genotypes of eight single nucleotide polymorphisms covering the aquaporin locus were tested for association with caries experience. Interaction with genes involved in enamel formation was tested. The association between enamel microhardness at baseline, after creation of artificial caries lesion, and after exposure to fluoride and the genetic markers in AQP5 was tested. Finally, AQP5 expression in human whole saliva, after exposure to fluoride in a mammary gland cell line, which is known to express AQP5, and in Wistar rats was also verified. Nominal associations were found between caries experience and markers in the AQP5 locus. Since these associations suggested that AQP5 may be inhibited by levels of fluoride in the drinking water that cause fluorosis, we showed that fluoride levels above optimal levels change AQP5 expression in humans, cell lines, and rats. We have shown that AQP5 is involved in the pathogenesis of caries and likely interacts with fluoride.Öğe Assessment of dehiscence and fenestration in children and adolescents using cone beam computed tomography(University Library System, University of Pittsburgh, 2021) Akbulut, Aslıhan; Ballı Akgöl, Beyza; Orhan, Kaan; Bayram, MerveObjective: To define the prevalence of dehiscence and fenestration and classify them in terms of the localization of fenestrations in a random sampled group of children and adolescent patients using cone-beam computed tomography (CBCT). Methods: CBCT performed at the Department of Oral and Maxillofacial Radiology of patients referred by the paediatric dentistry clinic were included in this retrospective study. Image evaluations were performed by dentomaxillofacial radiologist (AA, asst. prof.), and these images were examined in three dimensions of the axial, coronal, and sagittal planes. Intraexaminer agreement for the evaluations were found acceptable. The presence/absence of dehiscence and/or fenestration, fenestration's classification type, and localization of defects were recorded. Moreover, the presence/absence of periapical lesion in related root with dehiscence and fenestration was noted. For statistical analysis, The Chi-Square test, Fisher Freeman Halton Test, and Yates' Continuity of Correction were used. Results: 3061 roots in 1801 teeth of 120 cases were analyzed. The mean age was 9.97±2.22 years. Dehiscence was detected in 261(8.5%) roots of 161(8.9%) teeth, and fenestration was detected 63(2%) roots of 36(2%) teeth. The most common fenestration type was Type I, followed by Type II and IV. Dehiscence was observed more frequently in primary teeth than permanent teeth, and the difference was statistically significant (p:0.000). Dehiscence and fenestration incidence in maxillary teeth was significantly higher than in the mandibular teeth (pdehiscence:0.000, pfenestration:0.004). Apical lesions were observed more in primary teeth than permanent teeth for both defects. Conclusion: This study concludes that alveolar dehiscence and fenestrations are more common in primary teeth than permanent teeth. Moreover, these defects were detected more for the teeth in the maxilla. Concerning endodontic and orthodontic therapies in maxilla, use of CBCT is useful in determining the region's anatomical structure accurately in suspected cases of child and adolescent patients.Öğe Characterizing infraocclusion in primary molars: prevalence, accompanying findings, and infraocclusion severity and treatment implications(2024) Ballı Akgöl, Beyza; Üstün, Nilüfer; Bayram, MerveBackground: This manuscript investigates the prevalence, classification, accompanying findings, and treatment modalities associated with infraoccluded primary molars. The aim of this study categorizing primary molars based on the severity of infraocclusion and assessing their respective treatment interventions across different severity groups. Methods: The classification, treatment types, accompanying findings, and the condition of succeeding premolars of infraoccluded molars were documented. Chi-square tests, including Fisher’s Exact Chi-square test, Fisher Freeman Halton Exact Chi-square test, and One Sample Chi-square test, were conducted. The predetermined significance level was less than 0.05. Results: The study population consisted of 3132 subjects aged 3 to 15 years, with a prevalence of 4.3% for infraocclusion. Infraocclusion typically manifests between 6 and 9 years of age and predominantly affects mandibular primary molars. Treatment interventions varied based on infraocclusion severity, with more invasive procedures required for severe cases. Accompanying findings associated with infraocclusion include adjacent teeth tipping, significant deviation in midline shifts towards the affected side and increased caries. Additionally, succeeding premolar agenesis was observed in 2% of infraoccluded molars, with extraction rates higher in cases where the successor tooth was mesially or distally located. Conclusions: The study offers novel insights to dental practitioners concerning the severity and distribution of treatment interventions for infraocclusion. It suggests that more severe cases may necessitate more invasive procedures, with the aim of enhancing patient outcomes through timely intervention and personalized therapeutic strategies.Öğe Children's dental treatment requirements of first permanent molars with poor prognosis(Springer Heidelberg, 2022) Gürcan, Aliye Tuğçe; Bayram, MerveObjectives This retrospective study aimed to (i) survey the correlation between decayed, missing, filled teeth (DMFT), and presence of first permanent molars (FPMs) with poor prognosis and (ii) evaluate the treatment requirements. Materials and methods Seven hundred seventy-three children with fully erupted FPMs were included in this study. DMFT for the permanent dentition, FPMs, and Global DMFT were evaluated based on clinical and radiographic evaluation. The ratio of deep dentin caries (DDC) and apical lesion presence among FPMs, including treatment requirements, were analysed. Spearman rank correlation coefficient and t tests were used for statistical analysis. Results The caries prevalence was found at 61.4%, where the mean DMFT was calculated as 1.89 +/- 2.15. There was a positive correlation between DMFT values and age (r(s) = 0.27). On the other hand, there was a negative correlation between global DMFT values and age (r(s) = - 0.29). Regarding treatment needs of FPM with poor prognosis, 12.03% of the teeth needed pulpectomy, 8.93% pulpotomy, 8.93% pulp capping, and 5.3% extraction. Having higher DMFT values was correlated significantly (p < 0.01) with the presence of DDC (r(s) = 0.50) and apical lesion (r(s) = 0.34). Susceptibility to DDC and apical lesions was significantly higher at mandible than maxilla (p < 0.01). The correlation was significant between DMFT values and apical lesion presence (p < 0.01). Conclusion The ratio of FPMs with poor prognosis was found high in the study group. Treatment requirements of FPMs increased with age, and pulp interventions often took part in the majority. This study successfully concluded that higher DMFT values were correlated with the presence of DDC and apical lesion.Öğe Clinical and oral findings of a patient with Simpson-Golabi-Behmel syndrome(Springer, 2015) Bayram, Merve; Yıldırım, Mine; Seymen, FigenBackground The Simpson-Golabi-Behmel syndrome (SGBS) is an overgrowth condition characterised by macrosomia, mental deficiency, large head, prominent skull sutures, midface deficiency, hypertelorism, broad nose, wide mouth, macroglossia, malocclusion, highly arched palate, and musculoskeletal and limb abnormalities. The aim of this case report is to present clinical and oral findings of an 8-year-old boy who had been diagnosed with SGBS. Case report This patient had supernumerary nipples on the right side, cubitus valgus webbed fingers, scoliosis, umbilical hernia, a coarse face, macrocephaly, hypertelorism, a short broad nose, a wide mouth, a straight facial profile and hearing loss. The patient also had macroglossia, diastemas, over-retained primary tooth, absent mandibular permanent central incisors, and highly arched palate. Lateral cephalometric analysis revealed a large anterior cranial base, a large maxilla and mandible, a large inferior face height, and skeletal Class III jaw relationship. Follow-up After extraction of the over-retained primary central tooth, a partial prosthesis was fabricated in order to maintain function. The patient has been recalled regularly at 6-month intervals for 2 years. Over the following years the prosthesis was replaced due to facial growth. Conclusion Long term follow-up is essential for the patient with SGBS. Preventive dental care, including oral hygiene instructions, diet counselling and the use of fluoride has been implemented.Öğe Comparison of root canal length measurement methods in primary teeth(University Library System, University of Pittsburgh, 2018) Koruyucu, Mine; Bayram, Merve; Kasımo?lu, Yelda; Seymen, FigenObjectves: The purpose of this study was to evaluate the accuracy of conven5onal radiography, intraoral digital radi- ovisiography and electronic apex locator in determining the working length of root canals in primary teeth (in-vivo) and to compare the results with scanning electron microscopy measurements (ex-vivo). Materials and Methods: This study was conducted on 50 primary molar teeth. Standard endodon7c access cavity prepara7ons were prepared and the actual length was calculated by calibrated inves3gators. Working lengths were determined by using conven5onal radiography, RVG and apex locator in a total of 116 root canals. A;er the extraconal radiog- raphy in determining the working length in primary teeth. Conclusions: The electronic apex locators provide an ac- ceptable level of accuracy in determining root canal length in primary teeth. Clinical Relevance: The purpose of this study was to evaluate the accuracy of conven,onal radiography, intraoral digital radiovisiography and electronic apex locator in determining the working length of root canals in primary teeth and,to compare the results with scanning electron microscopy measurements.Öğe Erken çocukluk döneminde travmatik diş yaralanmalarının, maloklüzyonların ve diş çürüğünün ağız sağlığı ile ilişkili yaşam kalitesi üzerine etkisi(Selcuk University, 2021) Gürcan, Aliye Tuğçe; Koruyucu, Mine; Bayram, Merve; Seymen, FigenAmaç: Bu çalışmanın amacı, travmatik diş yaralanmalarının (TDY) ve diş çürüğünün okul öncesi çocukların Ağız Sağlığı ile İlişkili Yaşam Kalitesi (OHRQoL) üzerindeki etkisini değerlendirmektir. Gereç ve Yöntemler: Ağız Sağlığı ile İlişkili Yaşam Kalitesini ölçmek için 206 okul öncesi çocuğun velisine 13 soruluk Erken Çocukluk Çağı Ağız Sağlığı Etki Ölçeği (ECOHIS) uygulanmıştır. Çocukların travmatik diş yaralanmaları, diş çürüğü (dmft skorları) ve ön bölge maloklüzyon özelliklerine bakılmıştır. Bulgular: Çocukların yaş ortalaması 4.09 ± 0.97 bulunmuştur. Diş çürüğü, Ağız Sağlığı ile İlişkili Yaşam Kalitesi üzerinde olumsuz bir etki göstermiştir. Diş çürüğü; Ağız Sağlığı ile İlişkili Yaşam Kalitesi ortalaması, semptomları, fonksiyonu, psikolojisi, ailesel sıkıntı ve aile fonksiyon alanları üzerinde olumsuz bir etki göstermiştir. Ön bölgede maloklüzyon varlığı sadece sosyal etkileşim alanı üzerinde olumsuz bir etki göstermiştir. Komplike travmatik diş yaralanmaları, komplike olmayan travmatik diş yaralanmalarına göre semptomlar açısından daha olumsuz bir etki göstermiştir. Sonuç: Travmatik dental yaralanma ve maloklüzyon gözlenen dişin varlığı, okul öncesi çocukların Ağız Sağlığı ile İlişkili Yaşam Kalitesi üzerinde yalnızca çocuk alanında olumsuz bir etkiye sahiptir; ancak, diş çürüğü, hem çocuk alanında hem aile alanında Ağız Sağlığı ile İlişkili Yaşam Kalitesi ile güçlü bir ilişkiye sahiptir.Öğe Gene-environment interaction in molar-incisor hypomineralization(Public Library of Science, 2021) Bezamat, Mariana; Souza, Juliana Feltrin de; Silva, Fernanda M.F.; Corrêa, Emilly G.; Fatturi, Aluhê Lopes; Brancher, Joäo Armando; Carvalho, Flávia M.; Cavallari, Tayla; Bertolazo, Laís; Machado-Souza, Cleber; Koruyucu, Mine; Bayram, Merve; Racic, Andrea; Harrison, Benjamin M.; Sweat, Yan Yan; Letra, Ariadne; Studen-Pavlovich, Deborah A.; Seymen, Figen; Amendt, Brad A.; Iani Werneck, Renata; Costa, Marcelo C.; Modesto, Adriana M.; Vieira, Alexandre RezendeMolar incisor hypomineralization (MIH) is an enamel condition characterized by lesions ranging in color from white to brown which present rapid caries progression, and mainly affects permanent first molars and incisors. These enamel defects usually occur when there are disturbances during the mineralization or maturation stage of amelogenesis. Both genetic and environmental factors have been suggested to play roles in MIH’s development, but no conclusive risk factors have shown the source of the disease. During head and neck development, the interferon regulatory factor 6 (IRF6) gene is involved in the structure formation of the oral and maxillofacial regions, and the transforming growth factor alpha (TGFA) is an essential cell regulator, acting during proliferation, differentiation, migration and apoptosis. In this present study, it was hypothesized that these genes interact and contribute to predisposition of MIH. Environmental factors affecting children that were 3 years of age or older were also hypothesized to play a role in the disease etiology. Those factors included respiratory issues, malnutrition, food intolerance, infection of any sort and medication intake. A total of 1,065 salivary samples from four different cohorts were obtained, and DNA was extracted from each sample and genotyped for nine different single nucleotide polymorphisms. Association tests and logistic regression implemented in PLINK were used for analyses. A potential interaction between TGFA rs930655 with all markers tested in the cohort from Turkey was identified. These interactions were not identified in the remaining cohorts. Associations (p<0.05) between the use of medication after three years of age and MIH were also found, suggesting that conditions acquired at the age children start to socialize might contribute to the development of MIH.Öğe Genetic influences on dental enamel that impact caries differ between the primary and permanent dentitions(Wiley, 2015) Bayram, Merve; Deeley, Kathleen; Reis, Maria; Trombetta, Vanessa; Ruff, Timothy; Sencak, Regina; Hummel, Michael; Dizak, Piper; Washam, Kelly; Romanos, Helena; Lips, Andrea; Alves, Gutemberg; Costa, Marcelo; Granjeiro, Jose; Antunes, Leonardo; Kuechler, Erika; Seymen, Figen; Vieira, AlexandreClinically, primary and permanent teeth are distinct anatomically and the presentation of caries lesions differs between the two dentitions. Hence, the possibility exists that genetic contributions to tooth formation of the two dentitions are different. The purpose of this study was to test the hypothesis that genetic associations with an artificial caries model will not be the same between primary and permanent dentitions. Enamel samples from primary and permanent teeth were tested for microhardness at baseline, after carious lesion creation, and after fluoride application to verify association with genetic variants of selected genes. Associations were found between genetic variants of ameloblastin, amelogenin, enamelin, tuftelin, tuftelin interactive protein 11, and matrix metallopeptidase 20 and enamel from permanent teeth but not with enamel from primary teeth. In conclusion, our data continue to support that genetic variation may impact enamel development and consequently individual caries susceptibility. These effects may be distinct between primary and permanent dentitions.Öğe Genetic polymorphisms influence shear bond resistance of orthodontic brackets(Elsevier, 2020) Randazzo, Adam C.; Burnheimer, John M.; Bayram, Merve; Seymen, Figen; Vieira, Alexandre RezendeObjectives: The purpose of this study was to determine if shear bond resistance of orthodontic brackets bonded to enamel is associated with genes implicated in the enamel mineralization process.Methods: Ninety-two permanent, caries-free premolars extracted for orthodontic purposes and their associated saliva samples were obtained. Eighteen single nucleotide polymorphisms (SNPs) were studied for association with shear bond resistance. The genes of interest in this study were those previously associated with dental caries by our group. All tooth samples were bonded on the buccal surface with metallic lower lateral brackets, and then subjected to physical debonding. The force required to debond the bracket was recorded in Newtons (N) and converted to a shear bond resistance value in Megapascals (N/mm(2)). The data were analyzed for statistical significance as compared with the mean shear bond resistance value via PLINK whole genome analysis software.Results: Associations were found between the SNPs for tuftelin (rs7526319, P = 0.004) and amelogenin (rs17878486, P = 0.04) and a higher shear bond resistance.Conclusion: The collected data support the proposed hypothesis that genes involved in the mineralization process affect the bonding of orthodontic brackets, and such an association is of value for the field of orthodontics, particularly in evaluating the efficacy of enamel-resin bond strength for patients receiving treatment.Öğe In vitro acid-mediated initial dental enamel loss is associated with genetic variants previously linked to caries experience(Frontiers Media S.A., 2017) Vieira, Alexandre R.; Bayram, Merve; Seymen, Figen; Sencak, Regina C.; Lippert, Frank; Modesto, AdrianaWe have previously shown that AQP5 and BTF3 genetic variation and expression in whole saliva are associated with caries experience suggesting that these genes may have a functional role in protecting against caries. To further explore these results, we tested ex vivo if variants in these genes are associated with subclinical dental enamel mineral loss. DNA and enamel samples were obtained from 53 individuals. Enamel samples were analyzed for Knoop hardness of sound enamel, integrated mineral loss after subclinical carious lesion creation, and change in integrated mineral loss after remineralization. DNA samples were genotyped for single nucleotide polymorphisms using TaqMan chemistry. Chi-square and Fisher's exact tests were used to compare individuals above and below the mean sound enamel microhardness of the cohort with alpha of 0.05. The A allele of BTF3 rs6862039 appears to be associated with harder enamel at baseline (p = 0.09), enamel more resistant to demineralization (p = 0.01), and enamel that more efficiently regain mineral and remineralize (p = 0.04). Similarly, the G allele of AQP5 marker rs3759129 and A allele of AQP5 marker rs296763 are associated with enamel more resistant to demineralization (p = 0.03 and 0.05, respectively). AQP5 and BTF3 genetic variations influence the initial subclinical stages of caries lesion formation in the subsurface of enamel.Öğe Influence of COVID-19 pandemic on paediatric dental attendance(Springer Heidelberg, 2021) Üstün, Nilüfer; Ballı Akgöl, Beyza; Bayram, MerveObjectives: This retrospective study aimed to evaluate the effect of COVID-19 pandemic on dental attendance and emergency/non-emergency visits of children during the outbreak, compared to the same period of 2019. Materials and methods: Patients who visited the paediatric department clinic during the observed period were included in this study. The patient’s demographic data, purpose of visit, and treatment type were evaluated retrospectively from patient examination records. The Pearson chi-square and t-tests were used for comprehensive statistical analysis. Results: A total of 1454 patient files (1184/270 cases from non-pandemic/pandemic period) were assessed. A significant reduction was found in aggregated emergency/non-emergency visits during the pandemic period (p < 0.01). During the pandemic, average daily visits for emergency dental care were observed to have a decrease to half of the non-pandemic period. Non-emergency routine dental visits have also exhibited a drastic decline. Severe dental pain due to pulpal inflammation and abscess/swelling were the most frequently reported urgent dental complaints that patients applied to the paediatric dental clinic during the pandemic period. Conclusion: COVID-19 pandemic has a significant influence on patients’ attendance to paediatric dental clinic. Therefore, concerning a potential post-pandemic increase in treatment demand, meticulous future planning and proper regulation of dental care should be provided for better oral health and children’s quality of life. Clinical relevance: This study’s importance is the observation of dramatically reduced number of patient visits during the pandemic period which may yield increased number of oral health-related complications in the long run.Öğe Investigation of surface topography of different root-end filling materials: an in vitro study(University Library System, University of Pittsburgh, 2019) Koruyucu, Mine; Özcan, Hazal; Bayram, Merve; Çankaya, Abdullah Burak; Kekliko?lu, Nurullah; Seymen, FigenAim: Although there are many materials that can be used for retrograde filling in surgical endodontics, none of them can be regarded as an ideal material yet. The purpose of this study was to compare the surface topography of three different root-end filling materials. Methods: 36 extracted single rooted human incisor teeth were cleaned and decoronated to standardized 10 mm root lengths. The root segments were prepared and 2 mm apical resection were performed. The samples were randomly separeted to three groups (Group A: Ca(OH)2, Group B: MTA Angelus, Group C: ProRoot MTA), each one of them was comprised of 12 roots. Materials were placed as 2 mm apical barriers and obturated with guttapercha and AH-Plus sealer. Each group was dimidiated into two subgroups (A1,A2,B1,B2,C1,C2). Groups A1,B1,C1 were stored in normal saline (NS), groups A2,B2,C2 were stored in neutral phosphate buffer saline (NPBS) solution and samples were incubated at 370C for 2 weeks. Stereomicroscope (32X) was used to photograph the root-end filling. Results: All specimens demonstrated white crystals formation and sediment over the root-end filling materials and on the superficial border of the root-end cavities’ wall as a white plaque. A2,B2,C2 samples had more crystal sediment on root-end fillings than samples A1,B1,C1. Dissolution and corrosion were observed in groups A1, A2. Conclusions: The results of this study revealed that calcium hydroxide is more resorbable than MTA Angelus and ProRoot MTA. The crystals formation and precipitation were observed in neutral phosphate buffer saline solution which were more than normal saline solution for all groups as a hydroxiapatite crystals.Öğe Longevity of posterior composite restorations in children suffering from early childhood caries-results from a retrospective study(Springer Heidelberg, 2021) Bayram, Merve; Ballı Akgöl, Beyza; Üstün, NilüferObjectives This retrospective study aimed to evaluate health status as a new patient risk factor and analyze its influence on the survival of posterior composite restorations in patients with early childhood caries (ECC). Materials and methods Patients who received dental treatment of ECC under general anesthesia (GA) and attended at least one follow-up visit were included in this study. A total of 907 patient records were evaluated retrospectively through patient examination forms and panoramic radiographs. Kaplan-Meier survival probability analysis with log-rank test was used to assess the posterior composite restorations' longevity up to 24 months. Furthermore, risk factors were determined using Cox regression multivariate analysis. Results A total of 5063 posterior composite restorations were assessed. Following the Cox regression analysis to determine the effect of risk factors on longevity of composite restorations, findings revealed that the survival probability of composite fillings was significantly lower in patients with systemic disease (p= 0.00). Filling materials were compared based on the survival probabilities and results were further discussed. There was no significant relationship between age, gender, and the survival of the restoration; however, the child's health status (p= 0.00) and caries risk status (p= 0.05) significantly affected survival. Moreover, the type of arch and pulp intervention influenced the restoration's survival. Conclusion Systemic disease has a detrimental influence over longevity of composite restorations. Therefore, considering the reduced survival rates of the composite restorations for children who have systemic disease, alternative non-invasive treatment options should be considered.Öğe Mutations in RELT cause autosomal recessive amelogenesis imperfecta(Wiley, 2019) Kim, Jung-Wook; Zhang, Hong; Seymen, Figen; Koruyucu, Mine; Hu, Yuanyuan; Kang, Jenny; Kim, Youn Ju; Ikeda, Atsushi; Kasımoğlu, Yelda; Bayram, Merve; Zhang, Chuhua; Kawasaki, Kazuhiko; Bartlett, John D.; Saunders, Thomas L.; Simmer, James P.; Hu, Jan C-C.Amelogenesis imperfecta (AI) is a collection of isolated (non-syndromic) inherited diseases affecting dental enamel formation or a clinical phenotype in syndromic conditions. We characterized three consanguineous AI families with generalized irregular hypoplastic enamel with rapid attrition that perfectly segregated with homozygous defects in a novel gene: RELT that is a member of the tumor necrosis factor receptor superfamily (TNFRSF). RNAscope in situ hybridization of wild-type mouse molars and incisors showed specific Relt mRNA expression by secretory stage ameloblasts and by odontoblasts. Relt(-/-) mice generated by CRISPR/Cas9 exhibited incisor and molar enamel malformations. Relt(-/-) enamel had a rough surface and underwent rapid attrition. Normally unmineralized spaces in the deep enamel near the dentino-enamel junction (DEJ) were as highly mineralized as the adjacent enamel, which likely altered the mechanical properties of the DEJ. Phylogenetic analyses showed the existence of selective pressure on RELT gene outside of tooth development, indicating that the human condition may be syndromic, which possibly explains the history of small stature and severe childhood infections in two of the probands. Knowing a TNFRSF member is critical during the secretory stage of enamel formation advances our understanding of amelogenesis and improves our ability to diagnose human conditions featuring enamel malformations.Öğe Rethinking isolated cleft lip and palate as a syndrome(Elsevier Science Inc, 2018) Koruyucu, Mine; Kasımoğlu, Yelda; Seymen, Figen; Bayram, Merve; Patır Münevveroğlu, Aslı; Ergöz, Nihan; Tuna, Elif Bahar; Gencay, Koray; Deeley, Kathleen; Bussaneli, Diego; Modesto, Adriana; Vieira, Alexandre R.Objective. The goal of the present work was to use dental conditions that have been independently associated with cleft lip and palate (CL/P) as a tool to identify a broader collection of individuals to be used for gene identification that lead to clefts. Study design. We studied 1573 DNA samples combining individuals that were born with CL/P or had tooth agenesis, supernumerary teeth, molar incisor hypomineralization, or dental caries with the goal to identify genetic associations. We tested 2 single-nucleotide polymorphisms that were located in the vicinity of regions suggested to contribute to supernumerary teeth. Overrepresentation of alleles were determined for combinations of individuals as well as for each individual phenotypic group with an alpha of.05. Results. We determined that the allele C of rs622260 was overrepresented in all individuals studied compared with a group of unrelated individuals who did not present any of the conditions described earlier. When subgroups were tested, associations were found for individuals with hypomineralization. Conclusions. Although we did not test this hypothesis directly in the present study, based on associations reported previously, we believe that CL/P is actually a syndrome of alterations of the dentition, and considering it that way may allow for the identification of genotype-phenotype correlations that may be useful for clinical care.Öğe Role of estrogen related receptor beta (ESRRB) in DFN35B hearing impairment and dental decay(BMC, 2014) Weber, Megan L.; Hsin, Hong-Yuan; Kalay, Ersan; Brozkova, Dana Safka; Shimizu, Takehiko; Bayram, Merve; Deeley, Kathleen; Kuchler, Erika Calvano; Forella, Jessalyn; Ruff, Timothy; Trombetta, Vanessa M.; Sencak, Regina C.; Hummel, Michael; Briseno-Ruiz, Jessica; Revu, Shankar; Granjeiro, Jose Mauro; Antunes, Leonardo Dos Santos; Alves Antunes, Livia Azeredo; Abreu, Fernanda; Costa, Marcelo Castro; Tannure, Patricia Nivoloni; Koruyucu, Mine; Patır, Aslı; Poletta, Fernando; Mereb, Juan C.; Castilla, Eduardo Enrique; Orioli, Leda M.; Marazita, Mary L.; Ouyang, Hongjiao; Jayaraman, Thottala; Seymen, Figen; Vieira, Alexandre RezendeBackground: Congenital forms of hearing impairment can be caused by mutations in the estrogen related receptor beta (ESRRB) gene. Our initial linkage studies suggested the ESRRB locus is linked to high caries experience in humans. Methods: We tested for association between the ESRRB locus and dental caries in 1,731 subjects, if ESRRB was expressed in whole saliva, if ESRRB was associated with the microhardness of the dental enamel, and if ESRRB was expressed during enamel development of mice. Results: Two families with recessive ESRRB mutations and DFNB35 hearing impairment showed more extensive dental destruction by caries. Expression levels of ESRRB in whole saliva samples showed differences depending on sex and dental caries experience. Conclusions: The common etiology of dental caries and hearing impairment provides a venue to assist in the identification of individuals at risk to either condition and provides options for the development of new caries prevention strategies, if the associated ESRRB genetic variants are correlated with efficacy.
