dc.contributor.author | Sevimli, Gülşah | |
dc.contributor.author | Smith, Matthew J. | |
dc.contributor.author | Akgül Çağlar, Tuba | |
dc.contributor.author | Bilir, Şükriye | |
dc.contributor.author | Seçilmiş, Melike | |
dc.contributor.author | Altun, Hamza Y. | |
dc.contributor.author | Yiğit, Esra N. | |
dc.contributor.author | Yang, Fan | |
dc.contributor.author | Keeley, Thomas P. | |
dc.contributor.author | Malli, Roland | |
dc.contributor.author | Öztürk, Gürkan | |
dc.contributor.author | Mann, Giovanni E. | |
dc.contributor.author | Eroğlu, Emrah | |
dc.date.accessioned | 2022-05-20T11:36:24Z | |
dc.date.available | 2022-05-20T11:36:24Z | |
dc.date.issued | 2022 | en_US |
dc.identifier.citation | Sevimli, G., Smith, M. J., Akgül Çağlar, T., Bilir, Ş., Seçilmiş, M., Altun, H. Y. ... Eroğlu, E. (2022). Nitric oxide biosensor uncovers diminished ferrous iron-dependency of cultured cells adapted to physiological oxygen levels. Redox Biology, 53. https://doi.org/10.1016/j.redox.2022.102319 | en_US |
dc.identifier.issn | 2213-2317 | |
dc.identifier.uri | https://doi.org/10.1016/j.redox.2022.102319 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/9456 | |
dc.description.abstract | Iron is an essential metal for cellular metabolism and signaling, but it has adverse effects in excess. The physiological consequences of iron deficiency are well established, yet the relationship between iron supplementation and pericellular oxygen levels in cultured cells and their downstream effects on metalloproteins has been less explored. This study exploits the metalloprotein geNOps in cultured HEK293T epithelial and EA.hy926 endothelial cells to test the iron-dependency in cells adapted to standard room air (18 kPa O2) or physiological normoxia (5 kPa O2). We show that cells in culture require iron supplementation to activate the metalloprotein geNOps and demonstrate for the first time that cells adapted to physiological normoxia require significantly lower iron compared to cells adapted to hyperoxia. This study establishes an essential role for recapitulating oxygen levels in vivo and uncovers a previously unrecognized requirement for ferrous iron supplementation under standard cell culture conditions to achieve geNOps functionality. | en_US |
dc.description.sponsorship | Integration Projects of Sabanci University ; Heart Research U.K. ; British Heart Foundation ; European Cooperation in Science and Technology (COST) ; King's Together Strategic Award | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier B.V. | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Cell Culture | en_US |
dc.subject | Culture Media | en_US |
dc.subject | Ferric Iron | en_US |
dc.subject | Ferrous Iron | en_US |
dc.subject | GeNOps | en_US |
dc.subject | Hydrogen Peroxide | en_US |
dc.subject | Hyperoxia | en_US |
dc.subject | NO Bioavailability | en_US |
dc.subject | Normoxia | en_US |
dc.subject | Pericellular Oxygen | en_US |
dc.title | Nitric oxide biosensor uncovers diminished ferrous iron-dependency of cultured cells adapted to physiological oxygen levels | en_US |
dc.type | article | en_US |
dc.relation.ispartof | Redox Biology | en_US |
dc.department | İstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü | en_US |
dc.department | İstanbul Medipol Üniversitesi, Uluslararası Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı | en_US |
dc.authorid | 0000-0003-2033-860X | en_US |
dc.authorid | 0000-0002-6988-3636 | en_US |
dc.authorid | 0000-0001-9037-5217 | en_US |
dc.authorid | 0000-0003-0352-1947 | en_US |
dc.authorid | 0000-0002-9373-0808 | en_US |
dc.identifier.volume | 53 | en_US |
dc.relation.tubitak | info:eu-repo/grantAgreement/TUBITAK/SOBAG/118C242 | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.doi | 10.1016/j.redox.2022.102319 | en_US |
dc.institutionauthor | Akgül Çağlar, Tuba | |
dc.institutionauthor | Bilir, Şükriye | |
dc.institutionauthor | Yiğit, Esra N. | |
dc.institutionauthor | Öztürk, Gürkan | |
dc.institutionauthor | Eroğlu, Emrah | |
dc.identifier.wosquality | Q1 | en_US |
dc.identifier.wos | 000805648100002 | en_US |
dc.identifier.scopus | 2-s2.0-85129621442 | en_US |
dc.identifier.pmid | 35525027 | en_US |
dc.identifier.scopusquality | Q1 | en_US |