dc.contributor.author | Ghaffari Zaki, Asal | |
dc.contributor.author | Erdoğan, Yusuf Ceyhun | |
dc.contributor.author | Akgül Çağlar, Tuba | |
dc.contributor.author | Eroğlu, Emrah | |
dc.date.accessioned | 2022-03-18T08:01:41Z | |
dc.date.available | 2022-03-18T08:01:41Z | |
dc.date.issued | 2022 | en_US |
dc.identifier.citation | Ghaffari Zaki, A., Erdoğan, Y. C., Akgül Çağlar, T. ve Eroğlu, E. (2022). Chemogenetic approaches to dissect the role of H2O2 in redox-dependent pathways using genetically encoded biosensors. Biochemical Society Transactions, 50(1), 335-345. https://doi.org/10.1042/BST20210506 | en_US |
dc.identifier.issn | 0300-5127 | |
dc.identifier.issn | 1470-8752 | |
dc.identifier.uri | https://doi.org/10.1042/BST20210506 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12511/9139 | |
dc.description.abstract | Chemogenetic tools are recombinant enzymes that can be targeted to specific organelles and tissues. The provision or removal of the enzyme substrate permits control of its biochemical activities. Yeast-derived enzyme D-amino acid oxidase (DAAO) represents the first of its kind for a substrate-based chemogenetic approach to modulate H2O2 concentrations within cells. Combining these powerful enzymes with multiparametric imaging methods exploiting genetically encoded biosensors has opened new lines of investigations in life sciences. In recent years, the chemogenetic DAAO approach has proven beneficial to establish a new role for (patho)physiological oxidative stress on redoxdependent signaling and metabolic pathways in cultured cells and animal model systems. This mini-review covers established or emerging methods and assesses newer approaches exploiting chemogenetic tools combined with genetically encoded biosensors. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Portland Press Ltd | en_US |
dc.rights | info:eu-repo/semantics/embargoedAccess | en_US |
dc.subject | Chemogenetics | en_US |
dc.subject | Genetically Encoded Biosensors | en_US |
dc.subject | Reactive Nitrogen Species | en_US |
dc.subject | Reactive Oxygen Species | en_US |
dc.subject | Signalling | en_US |
dc.title | Chemogenetic approaches to dissect the role of H2O2 in redox-dependent pathways using genetically encoded biosensors | en_US |
dc.type | review | en_US |
dc.relation.ispartof | Biochemical Society Transactions | en_US |
dc.department | İstanbul Medipol Üniversitesi, Rektörlük, Sağlık Bilim ve Teknolojileri Araştırma Enstitüsü | en_US |
dc.authorid | 0000-0003-2033-860X | en_US |
dc.authorid | 0000-0002-9373-0808 | en_US |
dc.identifier.volume | 50 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.startpage | 335 | en_US |
dc.identifier.endpage | 345 | en_US |
dc.relation.tubitak | info:eu-repo/grantAgreement/TUBITAK/SOBAG/118C242 | |
dc.relation.publicationcategory | Diğer | en_US |
dc.identifier.doi | 10.1042/BST20210506 | en_US |
dc.institutionauthor | Akgül Çağlar, Tuba | |
dc.institutionauthor | Eroğlu, Emrah | |
dc.identifier.wosquality | Q2 | en_US |
dc.identifier.wos | 000743356700001 | en_US |
dc.identifier.scopus | 2-s2.0-85125554344 | en_US |
dc.identifier.pmid | 35015078 | en_US |
dc.identifier.scopusquality | Q1 | en_US |